Objective: The aim of this study is to evaluate the effects of dextrose prolotherapy on pain, activities of daily living and disability in patients with chronic non-specific low back pain. Material and Methods: Twenty patients with chronic non-specific low back pain refractory to conservative therapy were included in this study. The dextrose prolotherapy injections were administered to lumbar facet joints, lumbar and sacroiliac ligaments, respectively, at 0, 3, and 6 weeks. Level of activity and rest pain, activities of daily living and patient global assessment measured using a numeric rating scale, and the disabiliy level measured using the İstanbul Low Back Pain Disability Index (ILBPDI) were recorded at the baseline, and at 1, 3 and 6 months. Results: The rest pain, activity pain, activities of daily living, patient global assessment scale and ILBPDI scores were significantly improved at 1, 3 and 6 months compared to the pre-treatment values (p˂0.001). There was no significant difference with regard to all outcome measures in the third month scores when compared to the first month (p˃0.05). However, the activity pain, activities of daily living, patient global assessment scale and ILBPDI scores were significantly decreased at 6-month follow up when compared to 1-month follow up (p<0,05). Conclusion: Dextrose prolotherapy appears to be an effective method for relieving pain and in improving functions and activities of daily living for treating chronic non-specific low back pain over the six months period. Dextrose prolotherapy can be recommended as an effective, safe and economical treatment option for patients with chronic non-specific low back pain refractory to conservative therapy.