Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants
Yıl: 2021 Cilt: 9 Sayı: 10 Sayfa Aralığı: 1814 - 1818 Metin Dili: İngilizce DOI: 10.24925/turjaf.v9i10.1814-1818.4306 İndeks Tarihi: 08-06-2022
Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants
Öz: Iron (Fe) deficiency in plants is one of the widespread problems limiting agricultural production.
Generating crops more tolerant to Fe deficiency by genetic engineering or breeding is of great
interest but challenging due to the knowledge gaps in general plant Fe homeostasis. Although
several genes involved in Fe homeostasis have been identified, characterization of their roles is
mainly limited to specific organs at specific developmental stages of the plant, where their mutants
show the most striking phenotype. Vacuolar Iron Transporter 1 (VIT1) is a well-known gene that
has been characterized for its function in the mature seed of Arabidopsis thaliana. VIT1 is an Fe
transporter that determines the correct distribution of Fe storage in this organ. The study aimed to
explore new physiological functions for VIT1. As a first step, Arabidopsis thaliana plants that
contain PromoterVIT1: GUS constructs were used to study the temporal and spatial expression of
the gene throughout the plant’s lifecycle. GUS histochemical staining revealed the VIT1 promoter
is active in the mature leaves and mature reproductive organs. VIT1 promoter activity in the stamen
increased developmentally and was limited to tapetum and guard cells in the pollen sac. In the
female organ, VIT1 promoter activity increased as the pistil developed into a silique. Although all
the silique exhibited staining, staining density was higher in the peduncle, replum, and stigma
regions. Inside the developing silique, funicles were heavily stained. Furthermore, in silico analyses
of VT1 transcriptome and protein levels confirmed flower and the silique are hot spots for VT1
activity. Thus, the results may suggest a possible involvement of VT1 protein in several stages of
the reproductive system, specifically in the flowering and in the fruit developmen
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | eroglu s (2021). Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. , 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| Chicago | eroglu seckin Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. (2021): 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| MLA | eroglu seckin Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. , 2021, ss.1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| AMA | eroglu s Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. . 2021; 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| Vancouver | eroglu s Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. . 2021; 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| IEEE | eroglu s "Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants." , ss.1814 - 1818, 2021. 10.24925/turjaf.v9i10.1814-1818.4306 |
| ISNAD | eroglu, seckin. "Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants". (2021), 1814-1818. https://doi.org/10.24925/turjaf.v9i10.1814-1818.4306 |
| APA | eroglu s (2021). Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 9(10), 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| Chicago | eroglu seckin Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 9, no.10 (2021): 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| MLA | eroglu seckin Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.9, no.10, 2021, ss.1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| AMA | eroglu s Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2021; 9(10): 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| Vancouver | eroglu s Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2021; 9(10): 1814 - 1818. 10.24925/turjaf.v9i10.1814-1818.4306 |
| IEEE | eroglu s "Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 9, ss.1814 - 1818, 2021. 10.24925/turjaf.v9i10.1814-1818.4306 |
| ISNAD | eroglu, seckin. "Analysis of the VIT1 Promoter Activity in Developing Arabidopsis thaliana Plants". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 9/10 (2021), 1814-1818. https://doi.org/10.24925/turjaf.v9i10.1814-1818.4306 |
