Time and Concentration Dependent Effects of
Different Solvents on Proliferation of K562, HL60,
HCT-116 and H929 Cell Lines

KARABAY, Arzu Zeynep; OZKAN, Tulin; KOC, Asli; BUYUKBINGOL, Zeliha; AKTAN, Fugen

doi:10.29228/jrp.146

Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines

Asli KOC, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye.)

Arzu Zeynep KARABAY, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye.)

Tulin OZKAN, (Ankara Üniversitesi, Tıp Fakültesi Tıbbi Biyoloji Anabilim Dalı, Ankara, Türkiye)

Zeliha BUYUKBINGOL, (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye.)

Fugen AKTAN (Ankara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye.)

Journal of research in pharmacy (online)

11 0

Yıl: 2022 Cilt: 26 Sayı: 3 Sayfa Aralığı: 494 - 501 Metin Dili: İngilizce DOI: 10.29228/jrp.146 İndeks Tarihi: 08-06-2022

Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines

Öz:

Many organic solvents are used in cell culture models to dissolve polar and non-polar chemical compounds and drugs to test their activities. However, the solvents themselves can have cytotoxic effects on cells. The degree of cytotoxic effect may vary according to the solvent used. The aim of this study is to determine the time and concentration dependent effects of methanol, ethanol, dimethyl sulfoxide (DMSO), ethyl acetate and acetone on colorectal cancer (HCT-116), chronic myeloid leukemia (K562), multiple myeloma (H929) and acute promyelocytic leukemia (HL60) cell lines. According to our results, DMSO is the most toxic solvent and ethyl acetate and methanol are least toxic solvents in all cells. IC50 value of Acetone is greater than 10% in K562, HL60 and HCT-116 cells in all tested incubation times but in H929 cells IC50 value of acetone is decreased over time. In conclusion, since most of these solvents are currently being used for treatment of cells with different drugs and compounds in biochemical toxicology studies, it is essentially important to reveal their biochemical and toxic effects to determine the optimum concentration to be used.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KOC A, KARABAY A, OZKAN T, BUYUKBINGOL Z, AKTAN F (2022). Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. , 494 - 501. 10.29228/jrp.146
Chicago	KOC Asli,KARABAY Arzu Zeynep,OZKAN Tulin,BUYUKBINGOL Zeliha,AKTAN Fugen Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. (2022): 494 - 501. 10.29228/jrp.146
MLA	KOC Asli,KARABAY Arzu Zeynep,OZKAN Tulin,BUYUKBINGOL Zeliha,AKTAN Fugen Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. , 2022, ss.494 - 501. 10.29228/jrp.146
AMA	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. . 2022; 494 - 501. 10.29228/jrp.146
Vancouver	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. . 2022; 494 - 501. 10.29228/jrp.146
IEEE	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F "Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines." , ss.494 - 501, 2022. 10.29228/jrp.146
ISNAD	KOC, Asli vd. "Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines". (2022), 494-501. https://doi.org/10.29228/jrp.146

APA	KOC A, KARABAY A, OZKAN T, BUYUKBINGOL Z, AKTAN F (2022). Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. Journal of research in pharmacy (online), 26(3), 494 - 501. 10.29228/jrp.146
Chicago	KOC Asli,KARABAY Arzu Zeynep,OZKAN Tulin,BUYUKBINGOL Zeliha,AKTAN Fugen Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. Journal of research in pharmacy (online) 26, no.3 (2022): 494 - 501. 10.29228/jrp.146
MLA	KOC Asli,KARABAY Arzu Zeynep,OZKAN Tulin,BUYUKBINGOL Zeliha,AKTAN Fugen Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. Journal of research in pharmacy (online), vol.26, no.3, 2022, ss.494 - 501. 10.29228/jrp.146
AMA	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. Journal of research in pharmacy (online). 2022; 26(3): 494 - 501. 10.29228/jrp.146
Vancouver	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines. Journal of research in pharmacy (online). 2022; 26(3): 494 - 501. 10.29228/jrp.146
IEEE	KOC A,KARABAY A,OZKAN T,BUYUKBINGOL Z,AKTAN F "Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines." Journal of research in pharmacy (online), 26, ss.494 - 501, 2022. 10.29228/jrp.146
ISNAD	KOC, Asli vd. "Time and Concentration Dependent Effects of Different Solvents on Proliferation of K562, HL60, HCT-116 and H929 Cell Lines". Journal of research in pharmacy (online) 26/3 (2022), 494-501. https://doi.org/10.29228/jrp.146