A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism
Yıl: 2022 Cilt: 26 Sayı: 3 Sayfa Aralığı: 697 - 703 Metin Dili: İngilizce DOI: 10.29228/jrp.167 İndeks Tarihi: 08-06-2022
A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism
Öz: An overdose of an anticancer agent in the human body not only leads the high cytotoxicity on the
neoplastic cells but also causes serious side effects. The regular detection of an anticancer agent level in biological fluids
using an alternative technique is crucial in terms of assessment of therapeutic efficiency in chemotherapy process. In
this work, we developed a micelle-enhanced spectrofluorimetric approach for the determination of topotecan (TPC),
which is an effective anticancer agent used in the treatment of certain types of cancer, in human serum and binding
mechanism of TPC-DNA. The proposed method exhibited a strong pH-dependent emission signal at 535 nm after
excitation of 380 nm towards the TPC in the presence of surfactants. The relative fluorescence signal for TPC was found
to be linear in the wide concentration range of 0.01 – 1.8 μM (R2 = 0.9981) with a challenging detection limit of 3.3 nM.
The developed spectrofluorimetric method was successfully applied to the analysis of TPC in spiked human serum
samples with the good recovery results. Moreover, for the first time, the interaction mechanism between TPC and
double-stranded DNA (ds-DNA) was studied by developed spectrofluorimetric method. The binding constant value of
8.5 x 10-3 M-1 calculated by Stern-Volmer method indicated the strong intercalation-based binding of TPC into the base
pair of ds-DNA. The developed spectrofluorimetric method can provide new insight for the design of DNA-targeted
drugs, and lead an alternative approach for the detection of anticancer drugs such as TPC in biological samples.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ER E (2022). A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. , 697 - 703. 10.29228/jrp.167 |
| Chicago | ER Engin A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. (2022): 697 - 703. 10.29228/jrp.167 |
| MLA | ER Engin A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. , 2022, ss.697 - 703. 10.29228/jrp.167 |
| AMA | ER E A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. . 2022; 697 - 703. 10.29228/jrp.167 |
| Vancouver | ER E A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. . 2022; 697 - 703. 10.29228/jrp.167 |
| IEEE | ER E "A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism." , ss.697 - 703, 2022. 10.29228/jrp.167 |
| ISNAD | ER, Engin. "A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism". (2022), 697-703. https://doi.org/10.29228/jrp.167 |
| APA | ER E (2022). A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. Journal of research in pharmacy (online), 26(3), 697 - 703. 10.29228/jrp.167 |
| Chicago | ER Engin A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. Journal of research in pharmacy (online) 26, no.3 (2022): 697 - 703. 10.29228/jrp.167 |
| MLA | ER Engin A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. Journal of research in pharmacy (online), vol.26, no.3, 2022, ss.697 - 703. 10.29228/jrp.167 |
| AMA | ER E A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. Journal of research in pharmacy (online). 2022; 26(3): 697 - 703. 10.29228/jrp.167 |
| Vancouver | ER E A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism. Journal of research in pharmacy (online). 2022; 26(3): 697 - 703. 10.29228/jrp.167 |
| IEEE | ER E "A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism." Journal of research in pharmacy (online), 26, ss.697 - 703, 2022. 10.29228/jrp.167 |
| ISNAD | ER, Engin. "A sensitive surfactant-mediated spectrofluorimetric determination of chemotherapeutic agent topotecan in human serum and its investigation of DNA binding mechanism". Journal of research in pharmacy (online) 26/3 (2022), 697-703. https://doi.org/10.29228/jrp.167 |
