TY - JOUR TI - A Novel Yeast Isolated From Olive Mill Waste Candida tropicalis; Optimization of Medium Composition For Lipase Production AB - A novel microorganism that was isolated from an olive mill waste sample was screened for lipase production. This novel strain was identified and determined by 18S rDNA analysis and it was detected that the strain was 92% Candida tropicalis in ratio. Optimization of lipase production was carried out by the addition of nitrogen and carbohydrate sources into the lipase production medium as well as the effect of pH and temperature parameters were studied to increase the lipase production. Maximum growth conditions for the strain were detected at 4.0 pH medium and 30°C growth temperature. The effect of various nitrogen sources on lipase production showed that ammonium sulfate increased lipase production whereas urea, peptone and casein did not show a distinct effect. In addition presence of various sugars in the lipase production medium did not increase the lipase production efficiently although some oils did. The highest lipase activity was determined as 10.67 U/ml, with the addition of 1% ammonium sulfate and 1% olive oil into the production medium. AU - Cihangir, Nilufer AU - Kebabcı, Özgür DO - 10.30708.mantar.992551 PY - 2022 JO - MANTAR DERGİSİ VL - 13 IS - 1 SN - 2147-6845 SP - 8 EP - 14 DB - TRDizin UR - http://search/yayin/detay/517535 ER -