There is a few information about the genotoxic and cytotoxic effects of MTA Fillapex and AH Plus canal filling pastes in human pulp and pulp blood cells. And even that we know the there are some cytotoxic effects on the blood cells there is not enough data about the mechanism of these effects. Considering these reasons, in our study, it was aimed to investigate genotoxic and cytotoxic efficiency of the available materials by using the Cytokinesis-Blocked Micronucleus Cytome method. Material and Methods: 10 mL blood was obtained from ten healthy persons aged 20-35 years who did not use pharmaceuticals or were addicted to them on a regular basis. To the lymphocyte cultures derived from these samples, various quantities of MTA Fillapex and AH Plus channel fillers were applied. Micronucleus binuclear cells found, observed metaphase numbers, nucleoplasmic bridged (NPB) and nuclear bud cells were counted. After counting 1,000 cells in the samples, the presence of apoptotic and necrotic cells was also recorded for the statistical data. Results: Data were evaluated with nonparametric the Wilcoxon T-test. When the findings were evaluated, it was observed that the toxicity of MTA Fillapex increased with the dose. However, in all parameters except the NPB cells parameter, significantly less toxicity was detected compared to the positive control. After we observe data about NPB cells we found that there is no statistically significant difference between the MTA Fillapex group and positive control group also AH Plus study group showed toxicity during the polymerization period. Conclusion: MTA Fillapex paste showed less cytotoxic and genotoxic effect than AH Plus paste on the human blood cells.