Background: CD47, which is also known as integrin-associated protein, is a membrane protein of the immunoglobulin superfamily. Its expression was shown to be elevated in hematologic and many solid organ malignancies like pancreatic tumors. Materials and Methods: The materials of 80 patients diagnosed with endosonography (EUS)-guided fine needle aspiration biopsy (FNAB) and 42 patients diagnosed after resection were evaluated retrospectively. EUS guided FNAB specimens of all cases and slides prepared from blocks that had been selected from the subsequently obtained resection materials were stained with CD47 immunohistochemical staining. According to the CD47 expression level, it was divided into two groups as low and high, and the results were compared with clinicopathological and prognostic factors. Results: Eighty patients (male=36, female=44, mean age=61.32 years) were included in our study. In 80 EUS biopsy material, 53 (66.75%) cases were diagnosed with adenocarcinoma and this diagnosis was confirmed in resected patients. We found that 32 of 80 patients who underwent EUS-guided FNAB and 32 of those who underwent resection had positive staining with CD47. Considering high and low staining levels with CD47, our study showed a significant difference between high CD47 expression and disease-free survival in resection materials (p<0.005), but did not find a significant relationship between other clinicopathological prognostic factors. Conclusion: Although high CD47 expression levels were not detected in most EUS biopsy samples, it was observed that high CD47 expression had a negative prognostic effect on disease free survival