TY  - JOUR
TI  - Effects of betulinic acid on AKT/mTOR pathway in renal
cell carcinoma
AB  - Objective: Renal cancer is the most lethal among urological cancer. Treatments of renal cell carcinoma
(RCC) may be possible by immune checkpoint inhibitors and drug treatment targeting different molecules.
We aimed to determine the apoptotic effect of betulinic acid and its effects on expressions of apoptosisassociated
genes AKT-1 and mTOR in RCC cells.
Material and methods: In this study, we investigated the apoptotic activity of betulinic acid in CAKI-2 cell
line and its effect on AKT-1 and mTOR gene expression levels. In order to do so, following analyses were
conducted: WST-1 to identify the toxic effect of betulinic acid, Caspase-3/BCA to detect caspase enzyme
activity, Annexin-V and ELISA to determine for apoptotic effect, and finally, real-time PCR for expression
levels of AKT-1 and mTOR.
Results: Our study showed that different concentrations of betulinic acid induced apoptosis in renal cancer;
however, no effect was observed in healthy cells. In gene expression analysis, there was statistically significant
decrease in AKT-1 expression level while increasing mTOR expression level.
Conclusion: We suggested that betulinic acid with its apoptotic effect on RCC line and nontoxic effect on
healthy cell line and the effects on AKT/mTOR pathway may be a potential anticancer drug promising for
future studies.
AU  - ergen, arzu
AU  - ATAS, MERVE NUR
AU  - Cakmakoglu, Bedia
AU  - Ertugrul, Baris
AU  - Bireller, Sinem
DO  - 10.5152/tud.2022.21276
PY  - 2022
JO  - Turkish Journal of Urology
VL  - 48
IS  - 1
SN  - 2149-3235
SP  - 58
EP  - 63
DB  - TRDizin
UR  - http://search/yayin/detay/520187
ER  -