Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii

Emine YURTERİ, (Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Rize, Türkiye)
Merve AKSU, (Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Rize, Türkiye)
Haydar KUPLEMEZ, (Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Rize, Türkiye)
Aysel ÖZCAN, (Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Rize, Türkiye)
Fatih SEYİS (Recep Tayyip Erdoğan Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü, Rize, Türkiye)
Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi
7 0
Yıl: 2021 Cilt: 7 Sayı: 3 Sayfa Aralığı: 533 - 539 Metin Dili: İngilizce DOI: 10.24180/ijaws.946504 İndeks Tarihi: 29-07-2022

Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii

Öz:
The genus of Helichrysum comprises many species which have therapeutical effects and used in folk medicine. H. pallasii is one of these species, used in the region for traditional medicine and ornamental purposes. Therefore, optimizing alternative micropropagation protocols of tissue culture conditions and secondary metabolite production for these species needs attention. The effect of Thidiazuran (TDZ) combined with Zeatin (ZEA) on shoot regeneration in H. pallasi was investigated during this study. The leaf, stem and root parts taken from the seedling germinated under in vitro conditions were used as explants. As a result, the root explants were more efficient compared to other explants in inducing plant regeneration using1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (88.9%) and 1 mg L-1 TDZ treatment (85.7%). The lowest plant regeneration percentage (16.7%) was found in control medium using leaf explants.
Anahtar Kelime: micropropagation Thidiazuran Zeatin immortal flower tissue culture

Thiadiazuron ve Zeatin’in Helichrysum pallasii’de Bitki Rejenerasyonuna Etkisi

Öz:
Helichrysum cinsi, tedavi edici etkileri olan ve halk hekimliğinde kullanılan birçok türü içermektedir. H. pallasii yörede geleneksel tıp ve süs amaçlı kullanılan bu türlerden bir tanesidir. Bu nedenle, bu türler için doku kültüründe alternatif mikroçoğaltım protokollerinin ve sekonder metabolit üretiminin optimize edilmesi gerektirmektedir. Bu çalışmada Zeatin (ZEA) ile kombine edilmiş Thidiazuran (TDZ)’ nınH. pallasi'de sürgün rejenerasyonu üzerine etkileri incelenmiştir. İn vitro koşullarda çimlendirilen bitkiciklerden alınan yaprak, gövde ve kök kısımları eksplant olarak kullanılmıştır. Sonuç olarak, en yüksek bitki rejenerasyonu kök eksplantlarına uygulanan 1 mg L-1 Thidiazuran (TDZ) + 0.1 mg L-1 Zeatin (ZEA) (%88.9) ve 1 mg L-1 TDZ (%85.7) uygulamalarından elde edilmiştir. En düşük bitki rejenerasyon yüzdesi (%16.7) ise yaprak eksplantları kullanılan kontrol uygulamasından elde edilmiştir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA yurteri e, Aksu M, Küplemez H, ÖZCAN A, Seyis F (2021). Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. , 533 - 539. 10.24180/ijaws.946504
Chicago yurteri emine,Aksu Merve,Küplemez Haydar,ÖZCAN Aysel,Seyis Fatih Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. (2021): 533 - 539. 10.24180/ijaws.946504
MLA yurteri emine,Aksu Merve,Küplemez Haydar,ÖZCAN Aysel,Seyis Fatih Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. , 2021, ss.533 - 539. 10.24180/ijaws.946504
AMA yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. . 2021; 533 - 539. 10.24180/ijaws.946504
Vancouver yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. . 2021; 533 - 539. 10.24180/ijaws.946504
IEEE yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F "Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii." , ss.533 - 539, 2021. 10.24180/ijaws.946504
ISNAD yurteri, emine vd. "Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii". (2021), 533-539. https://doi.org/10.24180/ijaws.946504
APA yurteri e, Aksu M, Küplemez H, ÖZCAN A, Seyis F (2021). Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 7(3), 533 - 539. 10.24180/ijaws.946504
Chicago yurteri emine,Aksu Merve,Küplemez Haydar,ÖZCAN Aysel,Seyis Fatih Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi 7, no.3 (2021): 533 - 539. 10.24180/ijaws.946504
MLA yurteri emine,Aksu Merve,Küplemez Haydar,ÖZCAN Aysel,Seyis Fatih Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, vol.7, no.3, 2021, ss.533 - 539. 10.24180/ijaws.946504
AMA yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi. 2021; 7(3): 533 - 539. 10.24180/ijaws.946504
Vancouver yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii. Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi. 2021; 7(3): 533 - 539. 10.24180/ijaws.946504
IEEE yurteri e,Aksu M,Küplemez H,ÖZCAN A,Seyis F "Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii." Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi, 7, ss.533 - 539, 2021. 10.24180/ijaws.946504
ISNAD yurteri, emine vd. "Effects of Thidiazuran and Zeatin on Plant Regeneration in Helichrysum pallasii". Uluslararası Tarım ve Yaban Hayatı Bilimleri Dergisi 7/3 (2021), 533-539. https://doi.org/10.24180/ijaws.946504
Sayfa Oluşturulma Tarihi
23-04-2024 23:16

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