In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

Aybeniz YILDIRIM, (Marmara Üniversitesi Eczacılık Fakültesi Farmakognozi Anabilim Dalı, İstanbul, Türkiye)
Ali ŞEN, (Marmara Üniversitesi Eczacılık Fakültesi Farmakognozi Anabilim Dalı, İstanbul, Türkiye)
Fatih GÖGER, (Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Eskişehir, Türkiye)
Özlem BİNGÖL ÖZAKPINAR, (Marmara Üniversitesi, Eczacılık Fakültesi, Biyokimya Anabilim Dalı, İstanbul, Türkiye)
Leyla BİTİŞ (Marmara Üniversitesi Eczacılık Fakültesi Farmakognozi Anabilim Dalı, İstanbul, Türkiye)
Journal of research in pharmacy (online)
14 0
Yıl: 2022 Cilt: 26 Sayı: 1 Sayfa Aralığı: 163 - 173 Metin Dili: İngilizce DOI: 10.29228/jrp.113 İndeks Tarihi: 17-06-2022

In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz

Öz:
In this study, methanol extract (CSM) and their hexane (CSH), chloroform (CSC), ethyl acetate (CSEA) and aqueous methanol fractions (CSAM) prepared from aerial parts of Centaurea saligna (K.Koch) Wagenitz were investigated for in vitro antiproliferative, anti-inflammatory and antioxidant activity. Anticancer, antioxidant, antiinflammatory activities of these extracts were carried out by MTT, DPPH-ABTS, 5-lipoxygenase methods, respectively. Methanol extract and its fractions of C. saligna were evaluated for their cytotoxicity against MCF-7, A549, HeLa, HT-29, PC-3 cell lines at a concentration of 50 μg / ml. Total flavonoid and phenolic contents of extracts were detected by AlCI3 and Folin-Ciocalteu methods, respectively. Analysis of phytochemical of CSEA, showing a strong anti-inflammatory activity with good antioxidant activity, was performed by LC-MS/MS. CSC exhibited the best antiproliferative activity against HeLa, HT-29, MCF-7 cell lines with 50.18%, 46.88%, 45.42% mortality, respectively. CSEA showed the highest antioxidant activity with IC50 values of 82.05 μg/ml and 108.4 μg/ml against ABTS and DPPH radicals, respectively. The highest total phenolic amounts have been determined in CSEA with value of 379.2 mg GAE/g extract. In the same way, the highest total flavonoid amounts have been observed in CSEA with values of 170.3 mg QE/g extract. CSEA showed strong anti-inflammatory activity with IC50 value of 0.10 μg/ml when compared to indomethacine as standard (22.39 μg/ml). Analysis of CSEA by LC-MS/MS revealed that the major compounds were quinic acid, 5-caffeoylquinic acid, apigenin C-hexoside-C-pentoside, p-coumaroylquinic acid, quercetin glucoside, di-caffeoylquinic acid, isorhamnetine glucoside, isorhamnetin glucuronide and isorhamnetin derivative. These results proved that CSEA has significant anti-inflammatory and antioxidant activity and CSC has good antiproliferatif activity. Also, the results demostrate that CSEA and CSC are a good source for further bioactivity-guided isolation in discovering new active antioxidant, anti-inflammatory and antiproliferative compounds.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Yıldırım A, Sen A, Göger F, Bingöl Özakpınar Ö, Bitis L (2022). In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. , 163 - 173. 10.29228/jrp.113
Chicago Yıldırım Aybeniz,Sen Ali,Göger Fatih,Bingöl Özakpınar Özlem,Bitis Leyla In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. (2022): 163 - 173. 10.29228/jrp.113
MLA Yıldırım Aybeniz,Sen Ali,Göger Fatih,Bingöl Özakpınar Özlem,Bitis Leyla In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. , 2022, ss.163 - 173. 10.29228/jrp.113
AMA Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. . 2022; 163 - 173. 10.29228/jrp.113
Vancouver Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. . 2022; 163 - 173. 10.29228/jrp.113
IEEE Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L "In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz." , ss.163 - 173, 2022. 10.29228/jrp.113
ISNAD Yıldırım, Aybeniz vd. "In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz". (2022), 163-173. https://doi.org/10.29228/jrp.113
APA Yıldırım A, Sen A, Göger F, Bingöl Özakpınar Ö, Bitis L (2022). In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of research in pharmacy (online), 26(1), 163 - 173. 10.29228/jrp.113
Chicago Yıldırım Aybeniz,Sen Ali,Göger Fatih,Bingöl Özakpınar Özlem,Bitis Leyla In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of research in pharmacy (online) 26, no.1 (2022): 163 - 173. 10.29228/jrp.113
MLA Yıldırım Aybeniz,Sen Ali,Göger Fatih,Bingöl Özakpınar Özlem,Bitis Leyla In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of research in pharmacy (online), vol.26, no.1, 2022, ss.163 - 173. 10.29228/jrp.113
AMA Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of research in pharmacy (online). 2022; 26(1): 163 - 173. 10.29228/jrp.113
Vancouver Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz. Journal of research in pharmacy (online). 2022; 26(1): 163 - 173. 10.29228/jrp.113
IEEE Yıldırım A,Sen A,Göger F,Bingöl Özakpınar Ö,Bitis L "In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz." Journal of research in pharmacy (online), 26, ss.163 - 173, 2022. 10.29228/jrp.113
ISNAD Yıldırım, Aybeniz vd. "In vitro antiproliferative, antioxidant, anti-inflammatory activities and phenolic profile of Centaurea saligna (K.Koch) Wagenitz". Journal of research in pharmacy (online) 26/1 (2022), 163-173. https://doi.org/10.29228/jrp.113
Sayfa Oluşturulma Tarihi
23-04-2024 23:16

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