Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer

MOLLAOGLU, NUR; Ersoy, Halil Erhan; Acik, Leyla; Korkmaz, Mehmet Hakan; Gultekin, Sibel Elif; Bozdağ, Leyla; Bayır, Ömer

doi:10.54617/adoklinikbilimler.1091916

Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer

Leyla BOZDAĞ, (Gazi Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Leyla AÇIK, (Gazi Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Halil Erhan ERSOY, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Cerrahisi Anabilim Dalı, Ankara, Türkiye)

Ömer BAYIR, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Mehmet Hakan KORKMAZ, (Sağlık Bilimleri Üniversitesi, Dışkapı Yıldırım Beyazıt Eğitim ve Araştırma Hastanesi, Kulak Burun Boğaz Anabilim Dalı, Ankara, Türkiye)

Nur MOLLAOĞLU, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Ağız, Diş ve Çene Cerrahisi Anabilim Dalı, Ankara, Türkiye)

İpek ATAK (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Temel Bilimler Bölümü, Oral Patoloji Anabilim Dalı, Ankara, Türkiye)

ADO Klinik Bilimler Dergisi (online)

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Yıl: 2022 Cilt: 11 Sayı: 2 Sayfa Aralığı: 150 - 156 Metin Dili: İngilizce DOI: 10.54617/adoklinikbilimler.1091916 İndeks Tarihi: 29-07-2022

Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer

Öz:

Aim: This study aimed to investigate the potential role of the PDCD4 gene, involved in the progression of the oral potentially malign disorder (OPMD) and oral squamous cell carcinoma cancer (OSCC). Material and Methods: The study was conducted on saliva and blood samples that were collected from OSCC (n=6), OPMD (n=6) patients, and healthy (n=6) individuals. The expression of PDCD4 was determined by using qRT-PCR. Kruskal Wallis test was performed to estimate the relationship between PDCD4 expression and clinical characteristics of OSCC and OPMD patients. The correlation between PDCD4 expression and patients groups was tested with the Rank Spearman test. Results: PDCD4 mRNA expression levels were assessed in 6 OSCCs, 6 OPMD patients, and 6 healthy individuals saliva and blood. In both blood and saliva, PDCD4 mRNA expression levels were 6/6 (100%) underexpressed in OSCC, while it was underexpressed in 5/6 (83%) of OPMD. The mean value of PDCD4 was significantly downregulated in OPMD and OSCC than in healthy (p<0.05). The Spearman rank test obtained a p-value for the 2 tail test of 0.024 (p < 0.05), which showed a significant correlation between both, while the correlation coefficient of -0.530 showed a strong negative correlation of PDCD4 downregülation and OPMD, OSCC. Conclusion: PDCD4 expression level is correlated with OSCC and OPMD. This study indicates that PDCD4 expression levels in liquid biopsies may have potential as a diagnostic biomarker in OSCC and OPMD.

Anahtar Kelime: pdcd4 Oral squamous cell carcinoma Blood Oral potentially malign disorder saliva

Apoptoz ilişkili PDCD4: Oral kanserin erken tanısında umut vaadedici yeni bir biyobelirteç

Öz:

Amaç: Bu çalışma, oral potansiyel malign bozukluk (OPMD) ve oral skuamöz hücreli karsinom karsinomun (OSCC) ilerlemesinde rol oynayan PDCD4 geninin potansiyel rolünü araştırmayı amaçlamıştır. Gereç ve Yöntem: Çalışma OSCC (n=6), OPMD (n=6) ve sağlıklı (n=6) bireylerden alınan tükürük ve kan örnekleri üzerinde gerçekleştirildi. PDCD4'ün ifadesi, qRT-PCR kullanılarak belirlendi. PDCD4 ekspresyonu ile OSCC ve OPMD hastalarının klinik özellikleri arasındaki ilişkiyi tahmin etmek için Kruskal Wallis testi yapıldı. PDCD4 ekspresyonu ile hasta gruplarının korelasyonu Rank Spearman testi ile test edildi. Bulgular: PDCD4 mRNA ekspresyon seviyeleri 6 OSCC'de, 6 OPMD hastasında ve 6 sağlıklı bireyde tükürük ve kanda değerlendirildi. Hem kanda hem de tükürükte, PDCD4 mRNA ekspresyon seviyeleri OSCC'de 6/6 (%100) düşük ifade edilirken, OPMD'nin 5/6'sında (%83) düşük ifade edildi. Ortalama PDCD4 değeri, OPMD ve OSCC'de sağlıklı olandan önemli ölçüde düşük ifade edildi (p<0.05). Rank Spearman testi ile p= 0.024 (p < 0.05) OSCC ve OPMD arasında anlamlı bir korelasyon gösterirken; -0.530 korelasyon katsayısı, , PDCD4 ‘ün OPMD ve OSCC arasında güçlü bir negatif korelasyona sahip olduğunu gösterdi. Sonuç: PDCD4 ekspresyon seviyesi, OSCC ve OPMD ile ilişkilidir. Bu çalışma, sıvı biyopsilerdeki PDCD4 ekspresyon seviyelerinin OSCC ve OPMD'de tanısal bir biyobelirteç olma potansiyeline sahip olabileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Bozdağ L, Acik L, Ersoy H, Bayır Ö, Korkmaz M, MOLLAOGLU N, Gultekin S (2022). Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. , 150 - 156. 10.54617/adoklinikbilimler.1091916
Chicago	Bozdağ Leyla,Acik Leyla,Ersoy Halil Erhan,Bayır Ömer,Korkmaz Mehmet Hakan,MOLLAOGLU NUR,Gultekin Sibel Elif Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. (2022): 150 - 156. 10.54617/adoklinikbilimler.1091916
MLA	Bozdağ Leyla,Acik Leyla,Ersoy Halil Erhan,Bayır Ömer,Korkmaz Mehmet Hakan,MOLLAOGLU NUR,Gultekin Sibel Elif Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. , 2022, ss.150 - 156. 10.54617/adoklinikbilimler.1091916
AMA	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. . 2022; 150 - 156. 10.54617/adoklinikbilimler.1091916
Vancouver	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. . 2022; 150 - 156. 10.54617/adoklinikbilimler.1091916
IEEE	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S "Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer." , ss.150 - 156, 2022. 10.54617/adoklinikbilimler.1091916
ISNAD	Bozdağ, Leyla vd. "Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer". (2022), 150-156. https://doi.org/10.54617/adoklinikbilimler.1091916

APA	Bozdağ L, Acik L, Ersoy H, Bayır Ö, Korkmaz M, MOLLAOGLU N, Gultekin S (2022). Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi (online), 11(2), 150 - 156. 10.54617/adoklinikbilimler.1091916
Chicago	Bozdağ Leyla,Acik Leyla,Ersoy Halil Erhan,Bayır Ömer,Korkmaz Mehmet Hakan,MOLLAOGLU NUR,Gultekin Sibel Elif Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi (online) 11, no.2 (2022): 150 - 156. 10.54617/adoklinikbilimler.1091916
MLA	Bozdağ Leyla,Acik Leyla,Ersoy Halil Erhan,Bayır Ömer,Korkmaz Mehmet Hakan,MOLLAOGLU NUR,Gultekin Sibel Elif Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi (online), vol.11, no.2, 2022, ss.150 - 156. 10.54617/adoklinikbilimler.1091916
AMA	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi (online). 2022; 11(2): 150 - 156. 10.54617/adoklinikbilimler.1091916
Vancouver	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer. ADO Klinik Bilimler Dergisi (online). 2022; 11(2): 150 - 156. 10.54617/adoklinikbilimler.1091916
IEEE	Bozdağ L,Acik L,Ersoy H,Bayır Ö,Korkmaz M,MOLLAOGLU N,Gultekin S "Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer." ADO Klinik Bilimler Dergisi (online), 11, ss.150 - 156, 2022. 10.54617/adoklinikbilimler.1091916
ISNAD	Bozdağ, Leyla vd. "Apoptosis Related PDCD4: Promising Novel Biomarker Early Detection of Oral Cancer". ADO Klinik Bilimler Dergisi (online) 11/2 (2022), 150-156. https://doi.org/10.54617/adoklinikbilimler.1091916