Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics

Halil İbrahim GÜLER, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Trabzon, Türkiye)
Fulya AY ŞAL, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Zehra CAN, (Bayburt Üniversitesi, Uygulamalı Bilimler Yüksekokulu, Bayburt, Türkiye)
Yakup KARA, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Oktay YILDIZ, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Temel Eczacılık Bilimleri, Biyokimya Anabilim Dalı, Trabzon, Türkiye)
Ali Osman BELDÜZ, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Sabriye ÇANAKÇI, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Sevgi KOLAYLI (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Kimya Bölümü, Trabzon, Türkiye)
Turkish Journal of Biology
4 0
Yıl: 2021 Cilt: 45 Sayı: 4 Sayfa Aralığı: 530 - 548 Metin Dili: İngilizce DOI: 10.3906/biy-2104-5 İndeks Tarihi: 20-06-2022

Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics

Öz:
Propolis is a multi-functional bee product rich in polyphenols. In this study, the inhibitory effect of Anatolian propolis against SARS-coronavirus-2 (SARS-CoV-2) was investigated in vitro and in silico. Raw and commercial propolis samples were used, and both samples were found to be rich in caffeic acid, p-coumaric acid, ferulic acid, t-cinnamic acid, hesperetin, chrysin, pinocembrin, and caffeic acid phenethyl ester (CAPE) at HPLC-UV analysis. Ethanolic propolis extracts (EPE) were used in the ELISA screening test against the spike S1 protein (SARS-CoV-2): ACE-2 interaction for in vitro study. The binding energy values of these polyphenols to the SARS-CoV-2 spike and ACE-2 protein were calculated separately with a molecular docking study using the AutoDock 4.2.6 program. In addition, the pharmacokinetics and drug-likeness properties of these eight polyphenols were calculated according to the SwissADME tool. The binding energy value of pinocembrin was highest in both receptors, followed by chrysin, CAPE, and hesperetin. Based on the in silico modeling and ADME (absorption, distribution, metabolism, and excretion) behaviors of the eight polyphenols, the compounds exhibited the potential ability to act effectively as novel drugs. The findings of both studies showed that propolis has a high inhibitory potential against the Covid-19 virus. However, further studies are now needed.
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APA Güler H, Ay şal F, Can Z, KARA Y, YILDIZ O, BELDUZ A, CANAKCI S, Kolaylı S (2021). Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. , 530 - 548. 10.3906/biy-2104-5
Chicago Güler Halil Ibrahim,Ay şal Fulya,Can Zehra,KARA Yakup,YILDIZ OKTAY,BELDUZ Ali Osman,CANAKCI Sabriye,Kolaylı Sevgi Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. (2021): 530 - 548. 10.3906/biy-2104-5
MLA Güler Halil Ibrahim,Ay şal Fulya,Can Zehra,KARA Yakup,YILDIZ OKTAY,BELDUZ Ali Osman,CANAKCI Sabriye,Kolaylı Sevgi Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. , 2021, ss.530 - 548. 10.3906/biy-2104-5
AMA Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. . 2021; 530 - 548. 10.3906/biy-2104-5
Vancouver Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. . 2021; 530 - 548. 10.3906/biy-2104-5
IEEE Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S "Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics." , ss.530 - 548, 2021. 10.3906/biy-2104-5
ISNAD Güler, Halil Ibrahim vd. "Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics". (2021), 530-548. https://doi.org/10.3906/biy-2104-5
APA Güler H, Ay şal F, Can Z, KARA Y, YILDIZ O, BELDUZ A, CANAKCI S, Kolaylı S (2021). Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. Turkish Journal of Biology, 45(4), 530 - 548. 10.3906/biy-2104-5
Chicago Güler Halil Ibrahim,Ay şal Fulya,Can Zehra,KARA Yakup,YILDIZ OKTAY,BELDUZ Ali Osman,CANAKCI Sabriye,Kolaylı Sevgi Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. Turkish Journal of Biology 45, no.4 (2021): 530 - 548. 10.3906/biy-2104-5
MLA Güler Halil Ibrahim,Ay şal Fulya,Can Zehra,KARA Yakup,YILDIZ OKTAY,BELDUZ Ali Osman,CANAKCI Sabriye,Kolaylı Sevgi Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. Turkish Journal of Biology, vol.45, no.4, 2021, ss.530 - 548. 10.3906/biy-2104-5
AMA Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. Turkish Journal of Biology. 2021; 45(4): 530 - 548. 10.3906/biy-2104-5
Vancouver Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics. Turkish Journal of Biology. 2021; 45(4): 530 - 548. 10.3906/biy-2104-5
IEEE Güler H,Ay şal F,Can Z,KARA Y,YILDIZ O,BELDUZ A,CANAKCI S,Kolaylı S "Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics." Turkish Journal of Biology, 45, ss.530 - 548, 2021. 10.3906/biy-2104-5
ISNAD Güler, Halil Ibrahim vd. "Targeting CoV-2 spike RBD and ACE-2 interaction with flavonoids of Anatolian propolis by in silico and in vitro studies in terms of possible COVID-19 therapeutics". Turkish Journal of Biology 45/4 (2021), 530-548. https://doi.org/10.3906/biy-2104-5
Sayfa Oluşturulma Tarihi
23-04-2024 23:21

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