Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods
Yıl: 2022 Cilt: 38 Sayı: 1 Sayfa Aralığı: 7 - 16 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.359 İndeks Tarihi: 21-06-2022
Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods
Öz: Aim: In this study, it was aimed to determine the presence of Vibrio spp.,
Aeromonas spp., and Listeria spp. in finned fish and shrimps consumed in
Turkey and the antibiotic resistance profile of the isolates using disc diffusion
method.
Materials and Methods: In the research, 300 seafoods obtained at different
times were used as material. Following isolation by classical cultural method,
classical PCR was performed to confirm the isolates at genus level and to
identify at species level for pathogenic species.
Results: Finfish and shrimp samples were contaminated with Vibrio spp.,
Aeromonas spp. and Listeria spp. with the rate of 19.4% (33/170), 14.7%
(25/170), 4.1% (7/170) and 13.8% (18/130), 13.1% (17/130), 6.2% (8/130),
respectively. Twenty-nine (9.7%) and 9 (3.0%) of the Vibrio spp. isolates were
identified as V. parahaemolyticus and V. cholerae, respectively. Thirty (10%) of
the Aeromonas spp. isolates were detected as A. hydrophila. L. monocytogenes
and V. vulnificus was not detected in any of samples. Antibiotic resistance
profile of the V. parahaemolyticus, V. cholerae, A. hydrophila and Listeria spp.
isolates was streptomycin (71.4%), teicoplanin (71.4%), ampicillin (87.5%),
teicoplanin (75%); streptomycin (80%), ampicillin (75%), cefixime (75%),
penicillin (75%), sulfamethoxazole/trimethoprim (100%), tetracycline
(75%); erythromycin (93.8%), vancomycin (81.2%), amoxacillin/clavulanic
acid (78.6%), ampicillin (85.7%), cephalothin (92.9%), penicillin G (92.9%);
and cephalothin (37.5%-42.9%), erythromycin (37.5%-42.9%), penicillin G
(37.5%-42.9%), tetracycline (37.5%-42.9%), respectively.
Conclusion: As a result, it is thought that fish and shrimp consumed in Turkey
may be contaminated with pathogenic Vibrio and Aeromonas species, and
antibiotic-resistant isolates may pose a risk to public health.
Anahtar Kelime: Deniz ürünlerinde Vibrio spp., Aeromonas spp. ve Listeria spp.'nin varlığı ve antibiyotik duyarlılıkları
Öz: Amaç: Bu çalışmada, Türkiye’de tüketilen yüzgeçli balıklarda ve karideslerde
Vibrio spp., Aeromonas spp. ve Listeria spp. varlığının ve elde edilen izolatların
antibiyotik direnç profilinin disk difüzyon yöntemi kullanılarak belirlenmesi
amaçlanmıştır.
Gereç ve Yöntem: Araştırmada, farklı zamanlarda toplanan 300 adet deniz
ürünü materyal olarak kullanılmıştır. Klasik kültürel yöntemle izolasyonun
ardından, izolatları cins düzeyinde doğrulamak ve patojen türleri tanımlamak
için klasik PCR yapılmıştır.
Bulgular: Yüzgeçli balık ve karides örnekleri Vibrio spp., Aeromonas spp. ve
Listeria spp. ile sırasıyla %19,4 (33/170), %14,7 (25/170), %4,1 (7/170)
and %13,8 (18/130), %13,1 (17/130), %6,2 (8/130) oranında kontamine
bulunmuştur. Vibrio spp. izolatlarının 29 (%9,7)'u ve 9 (%3,0)’u sırasıyla
V. parahaemolyticus ve V. cholerae olarak tanımlanmıştır. Aeromonas
spp. izolatlarının 30 (%10)'u A. hydrophila olarak tespit edilmiştir.
Örneklerin hiçbirinde L. monocytogenes ve V. vulnificus saptanmamıştır. V.
parahaemolyticus, V. cholerae, A. hydrophila ve Listeria spp. izolatlarının
antibiyotik direnç profili sırası ile streptomisin (%71,4), teikoplanin (%71,4),
ampisilin (%87,5), teikoplanin (%75); streptomisin (%80), ampisilin (%75),
sefiksim (%75), penisilin (%75), sulfametoksazol/trimetoprim (%100),
tetrasiklin (%75); eritromisin (%93,8), vankomisin (%81,2), amoksasilin/
klavulanik asit (%78,6), ampisilin (%85,7), sefalotin (%92,9), penisilin G
(%92,9); ve sefalotin (%37,5-%42,9), eritromisin (%37,5-%42,9), penisilin G
(%37,5-%42,9), tetrasiklin (%37,5-%42,9) olarak tespit edilmiştir.
Öneri: Sonuç olarak, Türkiye'de tüketilen balık ve karideslerin patojen Vibrio
ve Aeromonas türleri ile kontamine olabilecekleri ve antibiyotik dirençli
izolatların halk sağlığı açısından risk oluşturabileceği düşünülmektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Abdollahzadeh E, Ojagh SM, Hosseini H, Irajian G, et al., 2016. Prevalence and molecular characterization of Listeria spp. and Listeria monocytogenes isolated from fish, shrimp, and cooked ready-to-eat (RTE) aquatic products in Iran. LWTFood Sci Technol, 73, 205-211.
- Ahmed HA, El Bayomi RM, Hussein MA, Khedr MH, et al., 2018. Molecular characterization, antibiotic resistance pattern and biofilm formation of Vibrio parahaemolyticus and Vibrio cholerae isolated from crustaceans and humans. Int J Food Microbiol, 274, 31-37.
- Andrews WH, Hammack TS, 2001. United States Food and Drug Administration (USFDA) Bacteriological Analytical Manual. Silver Spring, Md, USA. Food Sampling and Preparation of Sample Homogenate, Chapter 1. Avşar C, Berber İ, Yıldırım AK, 2016. Isolation and characterization of Vibrio spp. from anchovy and garfish in the Sinop province. Turk Hij Tecr Biyol Derg, 73(2), 121- 130.
- Azwai SM, Alfallani EA, Abolghait SK, Garbaj AM, et al., 2016. Isolation and molecular identification of Vibrio spp. by sequencing of 16S rDNA from seafood, meat and meat products in Libya. Open Vet J, 6(1), 36-43.
- Baker-Austin C, Oliver JD, Alam M, Ali A, et al., 2018. Vibrio spp. infections. Nat Rev Dis Primers, 4(1), 1-19.
- Bulduklu P, Özer S, 2007. An investigation of motil aeromonads and mesophilic aerobic bacteria count in rainbow trout (Oncorhynchus mykiss Walbaum, 1792) marketed in Mersin. EgeJFAS, 24(1), 97-102.
- Chen S, Ge B, 2010. Development of a toxR-based loopmediated isothermal amplification assay for detecting Vibrio parahaemolyticus. BMC Microbiol, 10, 41.
- Clinical and Laboratory Standards Institute (CLSI), 2016. Performance Standards for Antimicrobial Susceptibility Testing, CLSI Supplement M1000S. 26th ed, Wayne PA. Cocolin L, Rantsiou K, Iacumin L, Cantoni C, et al., 2002.
- Direct identification in food samples of Listeria spp. and Listeria monocytogenes by molecular methods. Appl Environ Microbiol, 68(12), 6273-6282.
- Deng Y, Xu L, Chen H, Liu S, et al., 2020. Prevalence, virulence genes, and antimicrobial resistance of Vibrio species isolated from diseased marine fish in South China. Sci Rep, 10(1), 1-8.
- Doğruer Y, Koç U, 2017. The prevalence of the motile Aeromonas species on the shrimps and squids consumed in Antalya. MJAVL, 7(1), 40‐46.
- Dutta C, Chowdhury S, Panigrahi AK, Sengupta C, 2016. Study of HACCP and microbial quality of seafood during processing in plants around Kolkata. Asian J Anim Sci, 11(2), 140-147.
- Elmahdi S, DaSilva LV, Parveen S, 2016. Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review. Food Microbiol, 57, 128-134.
- Fallah AA, Saei-Dehkordi SS, Mahzounieh M, 2013.
- Occurrence and antibiotic resistance profiles of Listeria monocytogenes isolated from seafood products and market and processing environments in Iran. Food Control, 34(2), 630-636.
- Furrer B, Candrian U, Hoefelein C, Luethy J, 1991. Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments . J Appl Microbiol, 70(5), 372-379.
- Gonzalez-Escalona N, Cachicas V, Acevedo C, Rioseco ML, et al., 2005. Vibrio parahaemolyticus diarrhea, Chile, 1998 and 2004. Emerg Infect Dis, 11, 129-131.
- Hoel S, Vadstein O, Jakobsen AN, 2019. The significance of mesophilic Aeromonas spp. in minimally processed readyto- eat seafood. Microorganisms, 7(3), 91.
- Irkin R, Korukluoğlu M, Tavşanlı H, 2007. Microbial properties of some sea products intended for export. Turk Hij Tecr Biyol Derg, 64(1), 26-30.
- ISO 11290-1, 2017. Microbiology of the fo od chainhorizontal method for detection and enumeration of Listeria monocytogenes and Listeria spp.-Part 1: Detection method. Chemin de la Voie Creuse, case postale 56, CH- 1211 Geneva 20, Switzerland.
- ISO/TS 21872–2, 2007. Microbiology of food and animal feeding stuffs — horizontal method for the detection of potentially enteropathogenic Vibrio spp. — Part 1: Detection of species other than Vibrio parahaemolyticus and Vibrio cholerae. Chemin de la Voie Creuse, case postale 56, CH-1211 Geneva 20, Switzerland.
- Jalali M, Abedi D, 2008. Prevalence of Listeria species in food products in Isfahan, Iran. Int J Food Microbiol, 122, 336– 340.
- Jamali H, Paydar M, Ismail S, Looi CY, et al., 2015. Prevalence, antimicrobial susceptibility and virulotyping of Listeria species and Listeria monocytogenes isolated from open-air fish markets. BMC Microbiol, 15(1), 144.
- Jones J, Benner Jr R, DePaola A, Hara-Kudo Y, 2013. Vibrio densities in the intestinal contents of finfish from coastal alabama. Agric Food Anal Bacteriol, 3, 186-194.
- Kaskhedikar M, Chhabra D, 2010. Multiple drug resistance in Aeromonas hydrophila isolates of fish. Food Microbiol, 28, 157-168.
- Khamesipour F, Moradi M, Noshadi E, Momeni Shahraki M, 2014. Detection of the prevalence of Aeromonas hyrophila in shrimp samples by Polymerase Chain Reaction (PCR) and cultural method in the Iran. J Biodivers Environ Sci, 4(2), 47-52.
- Kim HJ, Ryu JO, Lee SY, Kim ES, et al., 2015. Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics. BMC Microbiol, 15, 239.
- Kshirsagar DP, Brahmhhatt MN, Chatur YA, Sinha N, 2013. Studies on occurence and distribution of virulent strains of Vibrio parahaemolyticus in finfishes and prawns from different ecosystem of Gujarat (India). J Vet Public Health, 11(1), 07-10.
- Kumar S, Lekshmi M, Parvathi A, Nayak BB, Varela MF, 2017. Antibiotic resistance in seafood-borne pathogens, In: Food Borne Pathogens and Antibiotic Resistance, Ed; Singh OV, Wiley Blackwell Publishing, United States, pp; 397-410.
- Lee C, Cho JC, Lee SH, Lee DG, et al., 2002. Distribution of Aeromonas spp. as identified by 16S rDNA restriction fragment length polymorphism analysis in a trout farm. J Appl Microbiol, 93, 976-985.
- Letchumanan V, Pusparajah P, Tan LTH, Yin WF, et al., 2015. Occurrence and antibiotic resistance of Vibrio parahaemolyticus from shellfish in Selangor, Malaysia. Front Microbiol, 6, 1417.
- Lijon MB, Khatun MM, Islam A, Khatun MM, et al., 2015. Detection of multidrug resistance Aeromonas hydrophila in farm raised fresh water prawns. Adv Vet Anim Res, 2(4), 469-474.
- Malcolm TTH, Cheah YK, Radzi CWJWM, Kasim FA, et al., 2015. Detection and quantification of pathogenic Vibrio parahaemolyticus in shellfish by using multiplex PCR and loop-mediated isothermal amplification assay. Food Control, 47, 664-671.
- McLaughlin JB, DePaola A, Bopp CA, Martinek KA, et al., 2005. Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan Oysters. N Engl J Med, 353, 1463- 1470.
- Messelhäusser U, Colditz J, Thärigen D, Kleih W, et al., 2010. Detection and differentiation of Vibrio spp. in seafood and fish samples with cultural and molecular methods. Int J Food Microbiol, 142(3), 360-364.
- Noorlis A, Ghazali FM, Cheah YK, Tuan Zainazor TC, et al., 2011. Antibiotic resistance and biosafety of Vibrio cholerae and Vibrio parahaemolyticus from freshwater ish at retail level. Int Food Res J, 18(4), 1523-1530.
- Osman KM, Kappell AD, Fox EM, Orabi A, et al., 2020. Prevalence, pathogenicity, virulence, antibiotic resistance, and phylogenetic analysis of biofilm-producing Listeria monocytogenes isolated from different ecological niches in Egypt: Food, humans, animals, and environment. Pathogens, 9(1), 5.
- Palumbo SA, Abeyta C, Stelma G, 1992. Aeromonas hydrophila, In: Compendium of Methods for the Microbiological Examination of Foods, Ed; Vanderzant C, Splitttstoesser DF, Third edition, APHA, Washington DC, pp; 497-515.
- Persson S, Al-Shuweli S, Yapici S, Jensen JN, et al., 2015. Identification of clinical Aeromonas species by rpoB and gyrB sequencing and development of a multiplex PCR method for detection of Aeromonas hydrophila, A. caviae, A. veronii, and A. media. J Clin Microbiol, 53(2), 653-656. Popoff M, 1984. Genus III Aeromonas, Kluyver and Van Niel 1936, 398AL, In: Bergey’s manual of systematic bacteriology, Ed; Krieg NR, Holt JG, Williams&Wilkins, London, pp; 545-548.
- Raissy M, Moumeni M, Ansari M, Rahimi E, 2012. Antibiotic resistance pattern of some Vibrio strains isolated from seafood. Iran J Fish Sci, 11(3), 618-626.
- Ren CH, Hu CQ, Luo P, Wang QB, 2009. Sensitive and rapid identification of Vibrio vulnificus by loop-mediated isothermal amplification. Microbiol Res, 164, 514-521.
- Rodas-Suárez OR, Flores-Pedroche JF, Betancourt-Rule JM, Quiñones-Ramirez EI, et al., 2006. Occurrence and antibiotic sensitivity of Listeria monocytogenes strains isolated from oysters, fish, and estuarine water. Appl Environ Microbiol, 72(11), 7410-7412.
- Rosec JP, Causse V, Cruz B, Rauzier J, et al., 2012. The international standard ISO/TS 21872–1 to study the occurence of total and pathogenic Vibrio parahaemolyticus and Vibrio cholerae in seafood: ITS improvement by use of a chromogenic medium and PCR. Int J Food Microbiol, 157(2), 189-194.
- Scarano C, Spanu C, Ziino G, Pedonese F, et al., 2014. Antibiotic resistance of Vibrio species isolated from sparus aurata reared in Italian mariculture. New Microbiol, 37(3), 329-337.
- Stevens DL, Bisno AL, Chambers HF, Dellinger EP, et al., 2014. Practice guidelines for the diagnosis and management of skin and soft tissue infections: 2014 update by the Infectious Diseases Society of America. Clin Infect Dis, 59(2), e10-e52.
- Stratev D, Odeyemi OA, 2016. Antimicrobial resistance of Aeromonas hydrophila isolated from different food sources: A mini-review. J Infect Public Health, 9(5), 535- 544.
- Sudha S, Divya PS, Francis B, Hatha AA, 2012. Prevalence and distribution of Vibrio parahaemolyticus in finfish from Cochin (south India). Vet Ital, 48(269), e81.
- Teh CSJ, Chua KH, Thong KL, 2010. Simultaneous differential detection of human pathogenic and nonpathogenic Vibrio species using a multiplex PCR based on gyrB and pntA genes. J Appl Microbiol, 108, 1940-1945.
- Telli AE, Doğruer Y, 2019. Discrimination of viable and dead Vibrio parahaemolyticus subjected to low temperatures using propidium monoazide–quantitative loop mediated isothermal amplification (PMA-qLAMP) and PMA-qPCR. Microb Pathog, 132, 109-116.
- Thenmozhi S, Vijayalakshmi P, Moorthy K, Sureshkumar BT, 2015. Virulence and molecular characterization of Aeromonas spp. from fish samples using ARDRA technique. Int J Pharm Biol Sci, 6(1), 593-603.
- Vivekanandhan G, Savithamani K, Hatha AAM, Lakshmanaperumalsamy P, 2002. Antibiotic resistance of Aeromonas hydrophila isolated from marketed fish and prawn of South India. Int J Food Microbiol, 76(1-2), 165- 168.
- Vu TTT, Alter T, Huehn S, 2018. Prevalence of Vibrio spp. in retail seafood in Berlin, Germany. J Food Prot, 81(4), 593- 597.
- Wieczorek K, Osek J, 2017. Prevalence, genetic diversity and antimicrobial resistance of Listeria monocytogenes isolated from fresh and smoked fish in Poland. Food Microbiol, 64, 164-171.
- Xie T, Wu Q, Zhang J, Xu X, et al., 2017. Comparison of Vibrio parahaemolyticus isolates from aquatic products and clinical by antibiotic susceptibility, virulence, and molecular characterisation. Food Control, 71, 315-321.
- Yamaki S, Yamazaki K, 2018. Food-borne pathogens related to seafood products, In: Seafood Safety and Quality, Ed; Latiful B, Yamazaki K, CRC Press, Boca Raton, pp; 43-61.
- Zhu W, Zhou S, Chu W, 2020. Comparative proteomic analysis of sensitive and multi-drug resistant Aeromonas hydrophila isolated from diseased fish. Microb Pathog, 139, 103930.
| APA | BIÇER Y, Telli A, Turkal G, Kahraman H, DOGRUER Y (2022). Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. , 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| Chicago | BIÇER YUSUF,Telli A. Ezgi,Turkal Gamze,Kahraman Hatice Ahu,DOGRUER YUSUF Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. (2022): 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| MLA | BIÇER YUSUF,Telli A. Ezgi,Turkal Gamze,Kahraman Hatice Ahu,DOGRUER YUSUF Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. , 2022, ss.7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| AMA | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. . 2022; 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| Vancouver | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. . 2022; 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| IEEE | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y "Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods." , ss.7 - 16, 2022. 10.15312/EurasianJVetSci.2022.359 |
| ISNAD | BIÇER, YUSUF vd. "Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods". (2022), 7-16. https://doi.org/10.15312/EurasianJVetSci.2022.359 |
| APA | BIÇER Y, Telli A, Turkal G, Kahraman H, DOGRUER Y (2022). Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences, 38(1), 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| Chicago | BIÇER YUSUF,Telli A. Ezgi,Turkal Gamze,Kahraman Hatice Ahu,DOGRUER YUSUF Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences 38, no.1 (2022): 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| MLA | BIÇER YUSUF,Telli A. Ezgi,Turkal Gamze,Kahraman Hatice Ahu,DOGRUER YUSUF Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences, vol.38, no.1, 2022, ss.7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| AMA | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| Vancouver | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods. Eurasian Journal of Veterinary Sciences. 2022; 38(1): 7 - 16. 10.15312/EurasianJVetSci.2022.359 |
| IEEE | BIÇER Y,Telli A,Turkal G,Kahraman H,DOGRUER Y "Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods." Eurasian Journal of Veterinary Sciences, 38, ss.7 - 16, 2022. 10.15312/EurasianJVetSci.2022.359 |
| ISNAD | BIÇER, YUSUF vd. "Occurrence and antibiotic susceptibility of Vibrio spp., Aeromonas spp. and Listeria spp. in seafoods". Eurasian Journal of Veterinary Sciences 38/1 (2022), 7-16. https://doi.org/10.15312/EurasianJVetSci.2022.359 |
