TY  - JOUR
TI  - Vitamin B12 reduces the negative effects of nicotine on fetal
bone development in the rats
AB  - Objectives: The aim of this experimental rat study was to
investigate the effectiveness of vitamin B12 (Vit-B12) against the
teratogenic effects of nicotine use during pregnancy in the fetus.
Materials and methods: Eighteen Wistar-Albino pregnant rats
were divided into six groups (n=3, each) as Group 1 (control),
Group 2 (Vit-B12), Group 3 (nicotine 3 mg/kg/day), Group 4
(nicotine 3 mg/kg/day + Vit-B12), Group 5 (nicotine 6 mg/kg/day)
and Group 6 (nicotine 6 mg/kg/day + Vit-B12). Nicotine was
administered subcutaneously and Vit-B12 w as a dministered
intraperitoneally for 20 days. The skeletal systems of the embryos
were stained using the double-staining method. The total bone
length (TBL), the length of the ossified part, and the ossification
rate were calculated using the ImageJ program. In addition,
the weight and crown-rump length (CRL) of the embryos were
measured and recorded.
Results: C linical s hock e ffect w as o bserved i n a ll r ats
administered nicotine. A significant decrease in weight, CRL,
and TBL was measured in Group 3 (p=0.0081) and, particularly
in Group 5 (p=0.026), which were given only nicotine, compared
to other groups. In the morphometric measurements of Group
4 and Group 6 to which Vit-B12 was added, values close to the
control group were measured (p=0.173). The data of Group 6
were statistically significantly larger than Group 5 (p=0.007),
while it was statistically smaller than Group 1 (p=0.0038).
Conclusion: Nicotine use negatively affects the morphological
development of the embryo. On the other hand, Vit-B12 provides
benefits in reducing these negative effects.
AU  - GÜLER, Hatice
AU  - AL, Özge
AU  - NİSARİ, Mehtap
AU  - PAYAS, Ahmet
AU  - Gürbüz, Kaan
AU  - batın, sabri
AU  - Ertekin, Tolga
AU  - EKİNCİ, Yakup
AU  - DOĞAN, Kadirhan
AU  - Yılmaz, Halil
DO  - 10.52312/jdrs.2022.552
PY  - 2022
JO  - Joint diseases and related surgery
VL  - 33
IS  - 1
SN  - 2687-4784
SP  - 216
EP  - 224
DB  - TRDizin
UR  - http://search/yayin/detay/522481
ER  -