Non-obstruktif azoospermide micro-TESE:
Önemli yenilikler

Altay, Barış; Ergün, Kasım Emre

Non-obstruktif azoospermide micro-TESE: Önemli yenilikler

Kasım Emre ERGÜN, (Ege Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, İzmir, Türkiye)

Barış ALTAY (Ege Üniversitesi, Tıp Fakültesi, Üroloji Anabilim Dalı, İzmir, Türkiye)

Androloji Bülteni

5 1

Yıl: 2021 Cilt: 23 Sayı: 4 Sayfa Aralığı: 264 - 268 Metin Dili: Türkçe İndeks Tarihi: 21-06-2022

Non-obstruktif azoospermide micro-TESE: Önemli yenilikler

Öz:

Non-obstruktif azoospermili erkeklerde sperm elde etme için altın standart yöntem olarak uygulanan mikrodiseksiyon TESE (micro-TESE) yönteminde, ilk tanımlandığı 1998 yılından bugüne, sperm elde etme oranlarında önemli artışlar olmuştur. Preoperatif olarak micro-TESE başarısını öngörebilmek için özellikle ultrasonografi, testis spektroskopisi, seminal plazma ekstrasellüler vezikülleri gibi konularda güncel çalışmalar yapılmaktadır. Aynı zamanda da, cerrahi sırasında daha az doku hasarı ile daha başarılı sonuçlara ulaşmak için mini-insizyon micro-TESE, longitudinal tunika albuginea insizyonu gibi yeni teknikler geliştirilmeye çalışılmaktadır. Sperm toplanması aşamasında daha verimli sonuçlar için mikroakışkan sistemler gibi yeni teknolojilerin micro-TESE’de kullanımı umut verici gözükmektedir

Anahtar Kelime:

Micro-TESE in non-obstructive azoospermia: Major innovations

Öz:

In the microdissection TESE (micro-TESE) method, which is used as the gold standard method for sperm retrieval in men with nonobstructive azoospermia, since 1998, when it was first defined, there has been a significant increase in sperm retrieval rates. In order to predict the success of micro-TESE preoperatively, up-to-date studies are carried out, especially on ultrasonography, testicular spectroscopy, seminal plasma extracellular vesicles. At the same time, new techniques such as mini-incision micro-TESE and longitudinal tunica albuginea incision are being developed in order to achieve more successful results with less tissue damage during surgery. The use of new technologies such as microfluidic systems in micro-TESE seems promising for more efficient results at the sperm sorting stage.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Derleme Erişim Türü: Erişime Açık

1. Schlegel PN. Causes of azoospermia and their management. Reprod Fertil Dev 2004;16:561–72. [CrossRef]
2. Kumar R. Medical management of non-obstructive azoospermia. Clinics (Sao Paulo) 2013;68:75–9. [CrossRef] https://doi. org/10.6061/clinics/2013(Sup01)08
3. Devroey P, Liu J, Nagy Z, Goossens A, Tournaye H, Camus M, et al. Pregnancies after testicular sperm extraction and intracytoplasmic sperm injection in non-obstructive azoospermia. Hum Reprod 1995;10:1457–60. [CrossRef]
4. Schlegel PN, Li PS. Microdissection TESE: Sperm retrieval in non-obstructive azoospermia. Hum Reprod Update 1998;4:439. [CrossRef]
5. Deruyver Y, Vanderschueren D, van der Aa F. Outcome of microdissection TESE compared with conventional TESE in non-obstructive azoospermia: a systematic review. Andrology 2014;2:20–4. [CrossRef]
6. Bernie AM, Mata DA, Ramasamy R, Schlegel PN. Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive azoospermia: a systematic review and meta-analysis. Fertil Steril 2015;104:1099–103.e1–3. [CrossRef]
7. Schlegel PN, Sigman M, Collura B, De Jonge CJ, Eisenberg ML, Lamb DJ, et al. Diagnosis and treatment of infertility in men: AUA/ASRM guideline part II. Fertil Steril 2021;115:62–9. [CrossRef]
8. Hopps CV, Mielnik A, Goldstein M, Palermo GD, Rosenwaks Z, Schlegel PN. Detection of sperm in men with y chromosome microdeletions of the AZFa, AZFb and AZFc regions. Hum Reprod 2003;18:1660–5. [CrossRef]
9. Abdel Raheem A, Garaffa G, Rushwan N, De Luca F, Zacharakis E, AbdelRaheem T, et al. Testicular histopathology as a predictor of a positive sperm retrieval in men with non-obstructive azoospermia. BJU Int 2013;111:492–9. [CrossRef]
10. Khelaia A, Saker Z, Tsintsadze O, Managadze L. Nonobstructıve azoospermia, follicle-stimulating hormone as a marker of successful sperm retrieval. Georgian Med News 2015;249:34–7. https:// pubmed.ncbi.nlm.nih.gov/26719547/
11. Nariyoshi S, Nakano K, Sukegawa G, Sho T, Tsuji Y. Ultrasonographically determined size of seminiferous tubules predicts sperm retrieval by microdissection testicular sperm extraction in men with nonobstructive azoospermia. Fertil Steril 2020;113:97–104.e2. [CrossRef]
12. Zhang S, Du J, Tian R, Xie S, Li F, Li Z. Assessment of the use of contrast enhanced ultrasound in guiding microdissection testicular sperm extraction in nonobstructive azoospermia. BMC Urol 2018;18:48. [CrossRef]
13. Ntorkou A, Tsili AC, Astrakas L, Goussia A, Panopoulou E, Sofikitis N, Argyropoulou MI. In vivo biochemical investigation of spermatogenic status:1H-MR spectroscopy of testes with nonobstructive azoospermia. Eur Radiol 2020;30:4284–94. [CrossRef]
14. Çelik Ö, Hatırnaz Ş, Erşahin A, Başbuğ A, Yıldırım GY, Özener V, et al. Testis spectroscopy may predict sperm retrieval rate in men with non-obstructive azoospermia undergoing micro-TESE. A pilot study. J Turk Ger Gynecol Assoc 2020;21:70–8. [CrossRef]
15. Siciliano L, Marciano V, Carpino A. Prostasome-like vesicles stimulate acrosome reaction of pig spermatozoa. Reprod Biol Endocrinol 2008;6:5. [CrossRef]
16. Chen H, Xie Y, Li Y, Zhang C, Lv L, Yao J, et al. Outcome prediction of microdissection testicular sperm extraction based on extracellular vesicles piRNAs. J Assist Reprod Genet 2021;38:1429–39. [CrossRef]
17. Caroppo E, Colpi EM, Gazzano G, Vaccalluzzo L, Piatti E, D’Amato G, Colpi GM. The seminiferous tubule caliber pattern as evaluated at high magnification during microdissection testicular sperm extraction predicts sperm retrieval in patients with nonobstructive azoospermia. Andrology 2019;7:8–14. [CrossRef]
18. Ichioka K, Matsui Y, Terada N, Negoro H, Goto T, Ogawa O. Three-dimensional simulation analysis of microdissection testicular sperm extraction for patients with non-obstructive azoospermia. Andrology 2020;8:1214–21. [CrossRef]
19. Almajed W, Alharbi M, Zini A. Use of mini-incision microdissection testicular sperm extraction in men with cryptozoospermia and nonobstructive azoospermia. Andrology 2020;8:1136–42. [CrossRef]
20. Li P, Yao CC, Zhi EL, Xu Y, Wan Z, Jiang YC, et al. Modified stepwise mini-incision microdissection testicular sperm extraction: a useful technique for patients with a history of orchidopexy affected by non-obstructive azoospermia. J Zhejiang Univ Sci B 2020;21:87–92. [CrossRef]
21. Mangum CL, Patel DP, Jafek AR, Samuel R, Jenkins TG, Aston KI, et al. Towards a better testicular sperm extraction: novel sperm sorting technologies for non-motile sperm extracted by microdissection TESE. Transl Androl Urol 2020;9:206–14. [CrossRef]
22. Rappa KL, Rodriguez HF, Hakkarainen GC, Anchan RM, Mutter GL, Asghar W. Sperm processing for advanced reproductive technologies: Where are we today? Biotechnol Adv 2016;34:578– 87. [CrossRef]
23. Samuel R, Son J, Jenkins TG, Jafek A, Feng H, Gale BK, et al. Microfluidic System for Rapid Isolation of Sperm From Microdissection TESE Specimens. Urology 2020;140:70–6. [CrossRef]
24. Said TM, Agarwal A, Grunewald S, Rasch M, Glander HJ, Paasch U. Evaluation of sperm recovery following annexin V magneticactivated cell sorting separation. Reprod Biomed Online 2006;13:336–9. [CrossRef]
25. Komoda T, Matsunaga T. Chapter 6 - Biotechnological Study. In: Komoda T, Matsunaga T, editors. Biochemistry for Medical Professionals. Boston: Academic Press; 2015. p.75–92.
26. Mittal S, Mielnik A, Bolyakov A, Schlegel P, Paduch D. Pd68–01 Pilot Study Results Using Fluorescence Activated Cell Sorting of Spermatozoa from Testis Tissue: A Novel Method for Sperm Isolation after TESE. J Urol 2017;197:e139. https://www. auajournals.org/doi/pdf/10.1016/j.juro.2017.02.3129

APA	Ergün K, Altay B (2021). Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . , 264 - 268.
Chicago	Ergün Kasım Emre,Altay Barış Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . (2021): 264 - 268.
MLA	Ergün Kasım Emre,Altay Barış Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . , 2021, ss.264 - 268.
AMA	Ergün K,Altay B Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . . 2021; 264 - 268.
Vancouver	Ergün K,Altay B Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . . 2021; 264 - 268.
IEEE	Ergün K,Altay B "Non-obstruktif azoospermide micro-TESE: Önemli yenilikler ." , ss.264 - 268, 2021.
ISNAD	Ergün, Kasım Emre - Altay, Barış. "Non-obstruktif azoospermide micro-TESE: Önemli yenilikler ". (2021), 264-268.

APA	Ergün K, Altay B (2021). Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . Androloji Bülteni, 23(4), 264 - 268.
Chicago	Ergün Kasım Emre,Altay Barış Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . Androloji Bülteni 23, no.4 (2021): 264 - 268.
MLA	Ergün Kasım Emre,Altay Barış Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . Androloji Bülteni, vol.23, no.4, 2021, ss.264 - 268.
AMA	Ergün K,Altay B Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . Androloji Bülteni. 2021; 23(4): 264 - 268.
Vancouver	Ergün K,Altay B Non-obstruktif azoospermide micro-TESE: Önemli yenilikler . Androloji Bülteni. 2021; 23(4): 264 - 268.
IEEE	Ergün K,Altay B "Non-obstruktif azoospermide micro-TESE: Önemli yenilikler ." Androloji Bülteni, 23, ss.264 - 268, 2021.
ISNAD	Ergün, Kasım Emre - Altay, Barış. "Non-obstruktif azoospermide micro-TESE: Önemli yenilikler ". Androloji Bülteni 23/4 (2021), 264-268.