Introduction: Cutibacterium acnes, formerly Propionibacterium acne, is a low virulence, aerotolerant anaerobes, Gram-positive, non-spore forming, pleomorphic bacillus. It is one of the common causes of infection that adversely affects the clinical outcome of patients, especially in implant-related infections after shoulder joint surgery. The aims of this study are to determine the incidence of C. acnes in tissue samples which were taken during shoulder arthroscopy and to compare the clinical status of patients with culture results. Methods: Patients who had shoulder arthroscopy in our hospital between January 2016 and July 2016 were evaluated prospectively. The patient’s visual analog scale score, Quick-Dash score, and Constant score were recorded before surgery and at 6th month after surgery and they were compared. Two or four samples were taken according to the shoulder pathology. Then, all of the samples were plated on 5% sheep blood agar and MacConkey agar for 14 days. Culture results and patient outcome scores compared. Results: We have followed 39 patients who met the inclusion criteria for 6 months. Thirteen of the patients were male, and 26 were female. There were seven patients whose culture results were positive (17.9%). There was no statistically significant difference in the distribution of clinical scores according to the culture result. (Mann-Whitney U p & lt; 0.05). Discussion and Conclusion: Despite the pre-operative skin preparation and standard antibiotic prophylaxis, shoulder arthroscopy mostly causes C. acnes inoculation, especially in the subacromial region. On the other hand, there was no difference in the clinical outcomes whether the patients developed C. acnes in tissue cultures or not. In the literature, C. acnes is associated with persistent pain and arthrosis in the shoulder region, but the results obtained in 6 month follow-ups are not compatible with this hypothesis.