TY  - JOUR
TI  - Cytotoxic and Apoptotic Effects of Carmofur and
Vitamin C Combination on HCT116 Colon Cancer
Cells
AB  - Objective: In recent years, especially combined treatment options that will reduce the side effects of drugs have attracted attention.
Carmofur is a new potent agent being investigated in the treatment of cancer. This study aimed to investigate the cytotoxic and apoptotic
effects of carmofur+vitamin C combination in colon cancer cells.
Materials and Methods: Carmofur in the range of 7.8-250 µM, vitamin C in 7.8 µM-2mM and carmofur+vitamin C in different concentrations
were evaluated on HCT116 cells by MTT test. In addition, the cell death pathway was determined by an apoptosis-necrosis assay.
Results: The IC50 value of carmofur in HCT116 cells was 8 µM, vitamin C was not effective at low doses, and the IC50 value was 2.2 mM.
When carmofur+vitamin C were applied to HCT116 cells, 4 µM carmofur and 125 µM vitamin C together inhibited half of the cells (IC50),
while 4 µM carmofur and 2 mM vitamin C inhibited half of the cells similarly.
Conclusion: Vitamin C doubled the anticancer effect of carmofur. As a result, it was observed that vitamin C supplementation significantly
increased cell death in anticancer drug administration. In addition, it was determined that the addition of vitamin C significantly increased
the apoptotic activity.
AU  - Danışman, Ferdane
DO  - 10.26650/experimed.2021.1038579
PY  - 2022
JO  - EXPERIMED
VL  - 12
IS  - 1
SN  - 2630-6050
SP  - 6
EP  - 11
DB  - TRDizin
UR  - http://search/yayin/detay/523274
ER  -