In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L.
Essential Oil and its Major Component 1,8-Cineole

Demirci, Fatih; Karadağ, Ayşe Esra; BILTEKIN, SEVDE NUR; Demirci, Betül

doi:10.25135/rnp.265.21.05.2080

In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole

Fatih DEMİRCİ, (Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, 26470-Eskişehir, Türkiye)

Betül DEMİRCİ, (Anadolu Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, 26470-Eskişehir, Türkiye)

Ayşe Esra KARADAĞ, (İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, 34810- Beykoz, İstanbul, Türkiye)

Sevde Nur BİLTEKİN (İstanbul Medipol Üniversitesi, Eczacılık Fakültesi, Farmasötik Mikrobiyoloji Anabilim Dalı, 34810- Beykoz, İstanbul, Türkiye)

Records of Natural Products

16 2

Yıl: 2022 Cilt: 16 Sayı: 2 Sayfa Aralığı: 194 - 199 Metin Dili: İngilizce DOI: 10.25135/rnp.265.21.05.2080 İndeks Tarihi: 23-06-2022

In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole

Öz:

In this present study, Rosmarinus officinalis L. essential oil, and its major component 1,8-cineole was evaluated in vitro for angiotensin converting enzyme 2 (ACE2), as well as for 5-lipoxygenase (5-LOX) enzyme inhibitory activity. The essential oil composition was confirmed both by GC-FID and GC/MS, where 1,8-cineole (62.7%), α-pinene (12.6 %), and camphor (8.3 %) were identified as the main constituents. Activity studies were performed at concentrations of 20 µg/mL for essential oil, and 5 µg/mL for the major compound 1,8-cineole, which were compared experimentally with standards. The essential oil was evaluated using a fluorometric multiplate based enzyme inhibition kit, where the ACE2 inhibition of Rosmarinus aetheroleum was 20%, while the 5-LOX inhibition was observed as 81.1%, respectively. In addition, the major constituent 1,8-cineole also showed remarkable ACE2 inhibition with 89.2%, and 5-LOX inhibition with 37.2%, respectively. As a result, the cineole chemotype rosemary essential oil, and its major constituent 1,8-cineol may have antiviral potential applications against coronaviruses due to ACE2 enzyme inhibition with anti-inflammatory effects. Further in vivo studies are needed to confirm the efficacy of essential oils and their constituents.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Kısa Bildiri Erişim Türü: Erişime Açık

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APA	Demirci F, Demirci B, Karadağ A, BILTEKIN S (2022). In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. , 194 - 199. 10.25135/rnp.265.21.05.2080
Chicago	Demirci Fatih,Demirci Betül,Karadağ Ayşe Esra,BILTEKIN SEVDE NUR In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. (2022): 194 - 199. 10.25135/rnp.265.21.05.2080
MLA	Demirci Fatih,Demirci Betül,Karadağ Ayşe Esra,BILTEKIN SEVDE NUR In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. , 2022, ss.194 - 199. 10.25135/rnp.265.21.05.2080
AMA	Demirci F,Demirci B,Karadağ A,BILTEKIN S In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. . 2022; 194 - 199. 10.25135/rnp.265.21.05.2080
Vancouver	Demirci F,Demirci B,Karadağ A,BILTEKIN S In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. . 2022; 194 - 199. 10.25135/rnp.265.21.05.2080
IEEE	Demirci F,Demirci B,Karadağ A,BILTEKIN S "In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole." , ss.194 - 199, 2022. 10.25135/rnp.265.21.05.2080
ISNAD	Demirci, Fatih vd. "In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole". (2022), 194-199. https://doi.org/10.25135/rnp.265.21.05.2080

APA	Demirci F, Demirci B, Karadağ A, BILTEKIN S (2022). In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. Records of Natural Products, 16(2), 194 - 199. 10.25135/rnp.265.21.05.2080
Chicago	Demirci Fatih,Demirci Betül,Karadağ Ayşe Esra,BILTEKIN SEVDE NUR In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. Records of Natural Products 16, no.2 (2022): 194 - 199. 10.25135/rnp.265.21.05.2080
MLA	Demirci Fatih,Demirci Betül,Karadağ Ayşe Esra,BILTEKIN SEVDE NUR In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. Records of Natural Products, vol.16, no.2, 2022, ss.194 - 199. 10.25135/rnp.265.21.05.2080
AMA	Demirci F,Demirci B,Karadağ A,BILTEKIN S In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. Records of Natural Products. 2022; 16(2): 194 - 199. 10.25135/rnp.265.21.05.2080
Vancouver	Demirci F,Demirci B,Karadağ A,BILTEKIN S In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole. Records of Natural Products. 2022; 16(2): 194 - 199. 10.25135/rnp.265.21.05.2080
IEEE	Demirci F,Demirci B,Karadağ A,BILTEKIN S "In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole." Records of Natural Products, 16, ss.194 - 199, 2022. 10.25135/rnp.265.21.05.2080
ISNAD	Demirci, Fatih vd. "In vitro ACE2 and 5-LOX Inhibition of Rosmarinus officinalis L. Essential Oil and its Major Component 1,8-Cineole". Records of Natural Products 16/2 (2022), 194-199. https://doi.org/10.25135/rnp.265.21.05.2080