Yıl: 2022 Cilt: 19 Sayı: 1 Sayfa Aralığı: 45 - 50 Metin Dili: İngilizce DOI: 10.4274/tjod.galenos.2022.44845

Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study

Öz:
Objective: To investigate the impact of laparoscopic ovarian drilling (LOD) on the expression of endometrial NFκB p65 (Rel A) in women with clomiphene- resistant polycystic ovary syndrome (PCOS). Materials and Methods: The study group comprised 25 normal-weight women with PCOS undergoing LOD and 14 control women without PCOS. Endometrial NF-κB p65 levels evaluated before and after LOD following immunohistochemical staining. The semiquantitative method was used to evaluate the intensity of NF-κB p65 levels. NF-κB p65 was found to higher in the endometrium of patients with PCOS compared to controls. LOD leads to significant down-regulation in endometrial NF-κB p65 expression. NF-κB p65 expression of PCOS and fertile control were similar after LOD. After LOD, H-score values decreased approximately 3-fold. The H-score of the control subjects was lower than the preoperative and postoperative H-score values of the control women with ovarian cyst. Results: Expression of endometrial NF-κB p65 did not change following ovarian cystectomy. The laterality of the ovarian cyst did not cause any change in preoperative H-score values. Conclusion: By downregulating the endometrial NF-κB p65 expression LOD improved physiological inflammation in women with PCOS
Anahtar Kelime:

Konular:
Fen > Tıp > Kadın Hastalıkları ve Doğum

Ovaryen drilling PKOS hastalarında endometrial nükleer faktör κB p65 ekspresyonunu downregüle eder: Prospektif olgu kontrol çalışması

Öz:
Amaç: Bu çalışmanın amacı laparoskopik ovaryen drilling (LOD) işleminin klomifen sitrat rezistansı olan polikistik over sendromlu (PKOS) hastalarda endometriyal NFκB p65 (Rel A) ekspresyon düzeylerini etkileyip etkilemediğinin araştırılmasıdır. Gereç ve Yöntemler: Çalışma grubu LOD yapılan 25 tane normal kiloya sahip PKOS’li hastadan oluşmaktadır. Kontrol grubunda ise 7 tane PKOS olmayan sağlıklı fertil kadın ve 7 tane de benin kisti olan ancak endometriyoması olmayan toplam 14 hasta mevcuttur. Endometriyal NF-κB p65 ekspresyonu LOD öncesi ve sonrası mid-sekretuar fazda ölçülmüştür. Endometriyal örnekler immünohistokimyasal yöntemlerle boyanmıştır. Endometriyal NF-κB p65 ekspresyonu H-skor metodu ile değerlendirilmiştir Bulgular: Endometriyal NF-κB p65 ekspresyonu PKOS olan hastalarda LOD öncesi fertil hastalardan daha yüksek bulunmuştur. PKOS’li hastalarda LOD sonrası NF-κB p65 ekspresyonu belirgin olarak azalmıştır. LOD sonrası PKOS grubundaki hastalar ile fertil grupta NF-κB p65 ekspresyonu benzer bulunmuştur. LOD sonrası H-skoru 3 kat azalmıştır. Fertil grubun H-skoru pre-op ve post-op dönemde over kistli hasta grubunun H-skorundan düşük bulunmuştur. Yapılan benin kistektomi operasyonları sonrası endometrial NF-κB p65 ekspresyonunda belirgin değişiklik gösterilmemiştir. Ovaryen kist varlığı pre-operatif H-skor değerlerini etkilememektedir. Sonuç: LOD, klomifen sitrat rezistansı olan PKOS’li hastalarda endometrial NF-κB p65 ekspresyonunu azaltarak fizyolojik enflamasyonu ve reseptiviteyi iyileştirmektedir
Anahtar Kelime:

Konular:
Fen > Tıp > Kadın Hastalıkları ve Doğum
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA GÜNGÖR N, GÜNGÖR K, YURCİ A, CİL K, HATIRNAZ Ş (2022). Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology, 19(1), 45 - 50. 10.4274/tjod.galenos.2022.44845
Chicago GÜNGÖR Nur Dokuzeylül,GÜNGÖR Kağan,YURCİ Arzu,CİL Kaan,HATIRNAZ Şafak Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology 19, no.1 (2022): 45 - 50. 10.4274/tjod.galenos.2022.44845
MLA GÜNGÖR Nur Dokuzeylül,GÜNGÖR Kağan,YURCİ Arzu,CİL Kaan,HATIRNAZ Şafak Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology, vol.19, no.1, 2022, ss.45 - 50. 10.4274/tjod.galenos.2022.44845
AMA GÜNGÖR N,GÜNGÖR K,YURCİ A,CİL K,HATIRNAZ Ş Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology. 2022; 19(1): 45 - 50. 10.4274/tjod.galenos.2022.44845
Vancouver GÜNGÖR N,GÜNGÖR K,YURCİ A,CİL K,HATIRNAZ Ş Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study. Turkish Journal of Obstetrics and Gynecology. 2022; 19(1): 45 - 50. 10.4274/tjod.galenos.2022.44845
IEEE GÜNGÖR N,GÜNGÖR K,YURCİ A,CİL K,HATIRNAZ Ş "Ovarian drilling down-regulates endometrial nuclear factor-κB p65 expression in women with PCOS: A prospective case-control study." Turkish Journal of Obstetrics and Gynecology, 19, ss.45 - 50, 2022. 10.4274/tjod.galenos.2022.44845