TY  - JOUR
TI  - Influence of Lipoxin-A4 Treatment on Cytokine, Chemokine Genes 
Expression, and Phenotypic Distribution of Lymphocyte Subsets During 
Experimental Liver Fibrosis
AB  - Objective: Lipoxins are anti-inflammatory, pro-resolving molecules that are secreted by immune cells such 
as neutrophils and macrophages. Lipoxins are a metabolite of the arachidonic acid pathway that resolve 
inflammation in fibrotic liver by producing several anti-inflammatory molecules. In this study, phenotypic distribution activation markers of lymphocytes in the spleen and expression levels of chemokines (chemokine 
(C-X-C motif) receptor 3, chemokine (C-X-C motif) ligand 10) cytokines (interferon gamma, tumor necrosis factor alpha, interleukin-6, interleukin-10) in the liver of lipoxin A4-treated fibrotic mice were investigated.
Materials and methods: Liver fibrosis was induced in BALB/c mice by thioacetamide administration. Lipoxin 
A4 was administered during last 2 weeks of induction. Fibrosis level was determined by using Knodell scoring. 
Lymphocytes were identified by flow-cytometry. Expression levels of genes were measured by quantitative 
real time polymerase chain reaction in liver homogenates. 
Results: Lipoxin A4 treatment caused an elevation of T-lymphocyte percentage in the spleen. Interestingly, 
administration of lipoxin A4 significantly reduced B-lymphocyte population in spleen of fibrotic group. CD8+
cytotoxic T cell frequency significantly reduced in thioacetamide-induced mice; however, lipoxin A4 administration increased that percentage significantly. Lipoxin A4 treatment significantly reduced frequency of 
activated ($CD8^+$$CD69^+$) cytotoxic T cells. Expression levels of chemokines significantly reduced in the liver 
after lipoxin A4 treatment. While expression levels of interferon gamma, tumor necrosis factor alpha, and 
interleukin-6 significantly reduced in the liver after lipoxin A4 treatment, an anti-inflammatory cytokine interleukin-10 expression was almost at similar levels in all experimental groups.
Conclusion: Lipoxin A4 performs its anti-inflammatory effect by reducing the frequency of activated T cells 
and expression levels of chemokines cytokines responsible from inflammatory immune response in the liver.
AU  - KAYHAN, Başak
AU  - Aslan, Elçin Latife
AU  - karaca, zeynal mete
AU  - GÜL, MEHMET
DO  - 10.5152/eurasianjmed.2022.20030
PY  - 2022
JO  - Eurasian Journal of Medicine
VL  - 54
IS  - 1
SN  - 1308-8734
SP  - 27
EP  - 35
DB  - TRDizin
UR  - http://search/yayin/detay/524250
ER  -