Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis
Yıl: 2022 Cilt: 47 Sayı: 2 Sayfa Aralığı: 738 - 746 Metin Dili: İngilizce DOI: 10.17826/cumj.1091707 İndeks Tarihi: 29-07-2022
Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis
Öz: Purpose: This study aimed to investigate the protective effect of geraniol and vitamin C on the experimental hepatocellular carcinogenesis (HCC) model by inducing FL83B hepatocyte cell lines with diethylnitrosamine (DENA).
Materials and Methods: The cells prepared in the medium were incubated with DENA (5 μM), geraniol (5 μM), and vitamin C (50 μM) for 48 hours in an incubator at 37 °C and 5% CO2. Groups were designed as follows: Group 1 (Control), group 2 (DENA Control), group 3 (DENA+Geraniol), group 4 (DENA+Vitamin C), and group 5 (DENA+Geraniol+Vitamin C) on standard cell culture plates. Six plates from each experimental group were studied. After the homogenization was centrifuged, analyses of pathway mediators NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX were performed by the Elisa method.
Results: The expression of Bax, caspase-3, COX-2, NFkB, GADD153, AIF, and GRP78 increased in cancer cells when compared to group 1 and decreased in other groups where antiproliferative agents were applied. Bcl-2 expression is decreased when compared to group 1, and expression is increased in other groups where antiproliferative agents are applied.
Conclusion: There was a significant hepatoprotective effect in the groups administered geraniol+vitamin C on pathway mediators in a DENA-induced HCC model.
Anahtar Kelime: Geraniol ve vitamin C’nin dietilnitrozamin kaynaklı deneysel hepatoselüler karsinogenez üzerindeki etkisi
Öz: Amaç: Bu çalışmanın amacı dietilnitrozamin (DENA) ile FL83B hepatosit hücre hattını indükleyerek oluşturulan deneysel hepatosellüler karsinogenezis (HSK) modelinde geraniol ve vitamin C'nin koruyucu etkisini araştırmaktır.
Gereç ve Yöntem: Bu amaçla çalışmamızda besiyerinde hazırlanan hücreler DENA (5 μM), geraniol (5 μM) ve vitamin C (50 μM) ile 37 °C sıcaklıkta ve %5’lik CO2‘li etüvde 48 saat inkübe edildi. Hücreler inkübe edildikten sonra gruplar standart hücre kültürü plakalarında Grup 1 (Kontrol), grup 2 (DENA Kontrol), grup 3 (DENA+Geraniol), grup 4 (DENA+Vitamin C) ve grup 5 (DENA+Geraniol+Vitamin C) olmak üzere beş gruba ayrıldı. Her deney grubundan altı plaka çalışıldı. Homojenatlar santrifüj edildikten sonra yolak mediyatörlerinden NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX-2 analizleri Elisa metodu ile yapıldı.
Bulgular: Kanser hücrelerinde Bax, caspase-3, COX-2, NFkB, GADD153, AIF ve GRP78 ekspresyonu grup 1 ile kıyaslandığında artmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyonun azalmakta olduğu belirlenmiştir. Bcl-2 ekspresyonu grup 1 ile kıyaslandığında azalmakta, geraniol ve vitamin C’nin uygulandığı diğer gruplarda ekspresyon artmaktadır.
Sonuç: Bu çalışmada, DENA ile indüklenen HCC modelinde geraniol ve vitamin C uygulanan gruplarda yolak mediyatörleri aracılığıyla önemli bir hepatoprotektif etki olduğu gözlendi.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Taşkın Şenol G, kaplan h, Boyan N, Oğuz Ö, Şingirik E (2022). Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. , 738 - 746. 10.17826/cumj.1091707 |
| Chicago | Taşkın Şenol Gamze,kaplan halil mahir,Boyan Neslihan,Oğuz Özkan,Şingirik Ergin Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. (2022): 738 - 746. 10.17826/cumj.1091707 |
| MLA | Taşkın Şenol Gamze,kaplan halil mahir,Boyan Neslihan,Oğuz Özkan,Şingirik Ergin Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. , 2022, ss.738 - 746. 10.17826/cumj.1091707 |
| AMA | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. . 2022; 738 - 746. 10.17826/cumj.1091707 |
| Vancouver | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. . 2022; 738 - 746. 10.17826/cumj.1091707 |
| IEEE | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E "Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis." , ss.738 - 746, 2022. 10.17826/cumj.1091707 |
| ISNAD | Taşkın Şenol, Gamze vd. "Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis". (2022), 738-746. https://doi.org/10.17826/cumj.1091707 |
| APA | Taşkın Şenol G, kaplan h, Boyan N, Oğuz Ö, Şingirik E (2022). Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. Cukurova Medical Journal, 47(2), 738 - 746. 10.17826/cumj.1091707 |
| Chicago | Taşkın Şenol Gamze,kaplan halil mahir,Boyan Neslihan,Oğuz Özkan,Şingirik Ergin Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. Cukurova Medical Journal 47, no.2 (2022): 738 - 746. 10.17826/cumj.1091707 |
| MLA | Taşkın Şenol Gamze,kaplan halil mahir,Boyan Neslihan,Oğuz Özkan,Şingirik Ergin Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. Cukurova Medical Journal, vol.47, no.2, 2022, ss.738 - 746. 10.17826/cumj.1091707 |
| AMA | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. Cukurova Medical Journal. 2022; 47(2): 738 - 746. 10.17826/cumj.1091707 |
| Vancouver | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis. Cukurova Medical Journal. 2022; 47(2): 738 - 746. 10.17826/cumj.1091707 |
| IEEE | Taşkın Şenol G,kaplan h,Boyan N,Oğuz Ö,Şingirik E "Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis." Cukurova Medical Journal, 47, ss.738 - 746, 2022. 10.17826/cumj.1091707 |
| ISNAD | Taşkın Şenol, Gamze vd. "Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis". Cukurova Medical Journal 47/2 (2022), 738-746. https://doi.org/10.17826/cumj.1091707 |
