TY  - JOUR
TI  - Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis
AB  - Purpose: This study aimed to investigate the protective effect of geraniol and vitamin C on the experimental hepatocellular carcinogenesis (HCC) model by inducing FL83B hepatocyte cell lines with diethylnitrosamine (DENA).
Materials and Methods: The cells prepared in the medium were incubated with DENA (5 μM), geraniol (5 μM), and vitamin C (50 μM) for 48 hours in an incubator at 37 °C and 5% CO2. Groups were designed as follows: Group 1 (Control), group 2 (DENA Control), group 3 (DENA+Geraniol), group 4 (DENA+Vitamin C), and group 5 (DENA+Geraniol+Vitamin C) on standard cell culture plates. Six plates from each experimental group were studied. After the homogenization was centrifuged, analyses of pathway mediators NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX were performed by the Elisa method. 
Results: The expression of Bax, caspase-3, COX-2, NFkB, GADD153, AIF, and GRP78 increased in cancer cells when compared to group 1 and decreased in other groups where antiproliferative agents were applied. Bcl-2 expression is decreased when compared to group 1, and expression is increased in other groups where antiproliferative agents are applied. 
Conclusion: There was a significant hepatoprotective effect in the groups administered geraniol+vitamin C on pathway mediators in a DENA-induced HCC model.
AU  - Oğuz, Özkan
AU  - Boyan, Neslihan
AU  - Şingirik, Ergin
AU  - Taşkın Şenol, Gamze
AU  - kaplan, halil mahir
DO  - 10.17826/cumj.1091707
PY  - 2022
JO  - Cukurova Medical Journal
VL  - 47
IS  - 2
SN  - 2602-3032
SP  - 738
EP  - 746
DB  - TRDizin
UR  - http://search/yayin/detay/524586
ER  -