Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney

KÖSE, KADER; YAY, ARZU HANIM; SEYDEL, GONUL SEYDA; DAĞLI, FATMA; YAZICI, Cevat; Güntürk, İnayet

doi:10.17826/cumj.783088

Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney

İnayet GÜNTÜRK, (Niğde Ömer Halisdemir Üniversitesi, Niğde Zübeyde Hanım Sağlık Yüksekokulu, Ebelik Bölümü, Niğde, Türkiye)

Gonul Seyda SEYDE, (Niğde Ömer Halisdemir Üniversitesi, Sağlık Hizmetleri Daire Başkanlığı, Niğde, Türkiye)

Fatma DAĞLI, (Çetin Şen Bilim ve Sanat Merkezi, Kayseri, Türkiye)

ARZU HANIM YAY, (Erciyes Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Kayseri, Türkiye)

Cevat YAZICI, (Erciyes Üniversitesi, Tıp Fakültesi, Klinik Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Kader KÖSE (Erciyes Üniversitesi, Tıp Fakültesi, Klinik Biyokimya Anabilim Dalı, Kayseri, Türkiye)

Cukurova Medical Journal

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Yıl: 2022 Cilt: 47 Sayı: 2 Sayfa Aralığı: 519 - 525 Metin Dili: İngilizce DOI: 10.17826/cumj.783088 İndeks Tarihi: 29-07-2022

Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney

Öz:

Purpose: Cisplatin is one of the most potent and widely used chemotherapeutic agents for the treatment of a wide variety of solid organ cancers. However, due to various side-effects such as nephrotoxicity, its therapeutic applications are limited. In the current study, it was aimed to investigate the effects of N-acetylcysteine (NAC), which is an effective antioxidant and anti-inflammatory agent, on cisplatin-induced apoptosis in rat kidneys. Materials and Methods: Twentyfour male Wistar rats were separated into 4 equal groups: Control, NAC-250, cisplatin (CP), and CP+NAC groups. Rats in the experimental groups were treated with intraperitoneally (i.p.) single-dose cisplatin (10 mg/kg) and NAC (i.p., 250 mg/kg) for 3 days. Results: At the end of the experiment, nephrotoxicity was confirmed by blood urea nitrogen and creatinine levels, and the apoptotic changes were demonstrated by TdT-mediated deoxyuridine triphosphate nick-end labeling (TUNEL) and caspase-3 levels in rat kidneys. The number of TUNEL-positive cells and caspase-3 levels were significantly increased by cisplatin. Treating the rats with NAC significantly decreased TUNEL-positive cells and caspase-3 levels. Conclusion: These data suggest that apoptotic cell death is involved in the pathogenesis of cisplatin-induced nephrotoxicity, and that the inhibition of apoptosis plays a central role in the beneficial effects of NAC.

Anahtar Kelime: Cisplatin N-acetylcysteine Apoptosis

N-asetilsisteinin rat böbreklerinde sisplatin ile indüklenen apoptoz üzerine etkisi

Öz:

için en güçlü ve yaygın olarak kullanılan kemoterapötik ajanlardan biridirAncak nefrotoksisite gibi çeşitli yan etkileri nedeniyle terapötik uygulamaları sınırlıdır. Bu çalışmada, etkili bir antioksidan ve antienflamatuar ajan olan N-asetilsistein (NAC)’in rat böbreklerinde sisplatin kaynaklı apoptoz üzerindeki etkilerinin araştırılması amaçlanmıştır. Gereç ve Yöntem: Wistar cinsi erkek ratlar eşit sayıda olacak şekilde 4 gruba ayrıldı: Kontrol, NAC-250, sisplatin (CP) ve CP+NAC.Gruplardaki ratlara tek doz sisplatin (10 mg/kg) ve 3 gün NAC (250 mg/kg) intraperitoneal (i.p.) olarak uygulandı. Bulgular: Deneyin sonunda, nefrotoksisite, kan üre nitrojen ve kreatinin seviyeleri ile doğrulandı ve apoptotik değişiklikler, sıçan böbreklerinde TdT aracılı deoksiüridin trifosfat nick-end etiketleme (TUNEL) ve kaspaz-3 seviyeleri ile gösterildi. TUNEL pozitif hücre sayısı ve kaspaz-3 seviyeleri sisplatin ile önemli ölçüde arttı. Sıçanları NAC ile tedavi etmek, TUNEL pozitif hücreleri ve kaspaz-3 seviyelerini önemli ölçüde azalttı. Sonuç: Bu veriler, apoptotik hücre ölümünün sisplatin ile indüklenen nefrotoksisitenin patogenezinde etkili olduğunu ve apoptoz inhibisyonunun NAC'ın faydalı etkilerinde merkezi bir rol oynadığını göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Güntürk İ, SEYDEL G, DAĞLI F, YAY A, YAZICI C, KÖSE K (2022). Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. , 519 - 525. 10.17826/cumj.783088
Chicago	Güntürk İnayet,SEYDEL GONUL SEYDA,DAĞLI FATMA,YAY ARZU HANIM,YAZICI Cevat,KÖSE KADER Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. (2022): 519 - 525. 10.17826/cumj.783088
MLA	Güntürk İnayet,SEYDEL GONUL SEYDA,DAĞLI FATMA,YAY ARZU HANIM,YAZICI Cevat,KÖSE KADER Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. , 2022, ss.519 - 525. 10.17826/cumj.783088
AMA	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. . 2022; 519 - 525. 10.17826/cumj.783088
Vancouver	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. . 2022; 519 - 525. 10.17826/cumj.783088
IEEE	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K "Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney." , ss.519 - 525, 2022. 10.17826/cumj.783088
ISNAD	Güntürk, İnayet vd. "Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney". (2022), 519-525. https://doi.org/10.17826/cumj.783088

APA	Güntürk İ, SEYDEL G, DAĞLI F, YAY A, YAZICI C, KÖSE K (2022). Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. Cukurova Medical Journal, 47(2), 519 - 525. 10.17826/cumj.783088
Chicago	Güntürk İnayet,SEYDEL GONUL SEYDA,DAĞLI FATMA,YAY ARZU HANIM,YAZICI Cevat,KÖSE KADER Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. Cukurova Medical Journal 47, no.2 (2022): 519 - 525. 10.17826/cumj.783088
MLA	Güntürk İnayet,SEYDEL GONUL SEYDA,DAĞLI FATMA,YAY ARZU HANIM,YAZICI Cevat,KÖSE KADER Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. Cukurova Medical Journal, vol.47, no.2, 2022, ss.519 - 525. 10.17826/cumj.783088
AMA	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. Cukurova Medical Journal. 2022; 47(2): 519 - 525. 10.17826/cumj.783088
Vancouver	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney. Cukurova Medical Journal. 2022; 47(2): 519 - 525. 10.17826/cumj.783088
IEEE	Güntürk İ,SEYDEL G,DAĞLI F,YAY A,YAZICI C,KÖSE K "Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney." Cukurova Medical Journal, 47, ss.519 - 525, 2022. 10.17826/cumj.783088
ISNAD	Güntürk, İnayet vd. "Effect of N-acetylcysteine on cisplatin induced apoptosis in rat kidney". Cukurova Medical Journal 47/2 (2022), 519-525. https://doi.org/10.17826/cumj.783088