Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice

ARISOY, Münevver; KOLONKAYA, Nazif

Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice

Münevver ARISOY, (Ankara Üniversitesi, Sağlık Eğitim Fakültesi, Ankara, Türkiye)

Nazif KOLONKAYA (Hacettepe Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)

Turkish Journal of Biology

7 0

Yıl: 1998 Cilt: 22 Sayı: 4 Sayfa Aralığı: 427 - 434 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice

Öz:

Beyaz çürükçül funguslar içinde yer alan Phanerochaete chrysosporium ME446'un toksik, mutajenic ve karsinojenik özelliklere sahip olan çok sayıda organik bileşiği yıkma özelliğinden yola çıkarak; yine aynı özelliklere sahip olan ve organik klorlu insektisitler içinde yer alan heptaklorun (1, 4, 5, 6, 7, 8, 8-heptaklor -3a, 4, 7, 7a-tetrahidro- 4, 7- methanoin) söz konusu fungus ile yıkılmasında etkili fizyolojik koşullar saptanmıştır. Ayrıca heptaklor ve yıkımı sonucu oluşan metabolitlerinin fareler üzerinde oluşturabileceği toksik etkiler araştırılmıştır.Fizyolojik koşulların saptanmasına ilişkin çalışmalarda-, heptaklor yıkımında optimum pH S, inkübasyon süresi 20 gün, inkübasyon sıcaklığı 30°C -35°C ve heptaklor konsantrasyonu 50 mM olarak saptanmıştır. Optimum fizyolojik koşullar uygulandığında heptaklor için maksimum yıkım oranı %97.30 bulunmuştur. Toksikolojik değerlendirmeler ile ilgili çalışlarda öncelikle heptaklorun LD50 dozu saptanmış olup, farelerin kanındaki şekilli elemanlar üzerine etkisi incelenmiştir. Heptaklor için LD50 dozu 97.50: mg/kg bulunmuştur. Farelerde %50 ölüme neden olan konsantrasyondaki heptaklorun uygun fizyolojik koşullarda yıkımı ile oluşan metabolitler farelere uygulandığında, farelerin hiçbirinde ölüme yol açmadığı görülmüştür. Ayrıca heptaklor farelere doğrudan uygulandığında kanın şekilli elemanları üzerine niceliksel bir etki gösterirken, metabolitlerin farklı bir etki göstermediği gözlenmiştir.

Anahtar Kelime: fareler biyodegradasyon heptaklor organoklorlu böcek öldürücüler beyaz çürükçül mantarlar ölüm oranı metabolitler toksisite Phanerochaete chrysosporium

Konular: Biyoloji

Heptaklor'un Phanerochaete chrysosporium ME 446 ile biyolojik yıkımı: Heptaklor ve metabolitlerinin fareler üzerine toksik etkisi

Öz:

On the basis of degradation properties of Phanerochaete chroysosporium ME 446 found in white-rot fungi in respect of many organic compounds with toxic, mutagenic and carcinogenic characteristics, the physiological conditions effective for the degradation of heptachlor (1, 4, 5, 6, 7, 8, 8-heptachloro-3a, 4, 7, 7a-tetrahydro-4, 7-methanoindene), an organic chlorine used in insecticides Phanerochaete chrysosporium ME were determined. The potential toxic effects of heptachlor and metabolites on mice were also investigated. For the degradation of heptachlor, the optimum pH was found to be 5, incubation period 20 days, and incubation temperature, 30°C-35°C, while the optimum concentrations was 50 $mu$ M. The maximum heptachlor degradation rate under optimum physiological conditions was found to be 97.30%. The LD50 dose of heptachlor was determined, and its effects on cells in the blood of mice were investigated. The LD50 dose was found to be 97.50 mg/kg for heptachlor. Although this dose normally produces a death rate of 50% in mice, the resultant metabolites obtained through Phanerocheate chrysosporium ME 446 treatment caused no deaths. While direct application of heptachlor produced quantitive effects in the blood cells, no significant effects of heptachlor metabolites could be detected.

Anahtar Kelime: white rot fungi mortality metabolites toxicity Phanerochaete chrysosporium mice biodegradation heptachlor organochlorine insecticides

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ARISOY M, KOLONKAYA N (1998). Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. , 427 - 434.
Chicago	ARISOY Münevver,KOLONKAYA Nazif Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. (1998): 427 - 434.
MLA	ARISOY Münevver,KOLONKAYA Nazif Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. , 1998, ss.427 - 434.
AMA	ARISOY M,KOLONKAYA N Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. . 1998; 427 - 434.
Vancouver	ARISOY M,KOLONKAYA N Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. . 1998; 427 - 434.
IEEE	ARISOY M,KOLONKAYA N "Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice." , ss.427 - 434, 1998.
ISNAD	ARISOY, Münevver - KOLONKAYA, Nazif. "Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice". (1998), 427-434.

APA	ARISOY M, KOLONKAYA N (1998). Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. Turkish Journal of Biology, 22(4), 427 - 434.
Chicago	ARISOY Münevver,KOLONKAYA Nazif Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. Turkish Journal of Biology 22, no.4 (1998): 427 - 434.
MLA	ARISOY Münevver,KOLONKAYA Nazif Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. Turkish Journal of Biology, vol.22, no.4, 1998, ss.427 - 434.
AMA	ARISOY M,KOLONKAYA N Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. Turkish Journal of Biology. 1998; 22(4): 427 - 434.
Vancouver	ARISOY M,KOLONKAYA N Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice. Turkish Journal of Biology. 1998; 22(4): 427 - 434.
IEEE	ARISOY M,KOLONKAYA N "Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice." Turkish Journal of Biology, 22, ss.427 - 434, 1998.
ISNAD	ARISOY, Münevver - KOLONKAYA, Nazif. "Biodegradation of heptachlor by Phanerochaete chrysosporium ME 446: The toxic effects of heptachlor and its metabolites on mice". Turkish Journal of Biology 22/4 (1998), 427-434.