TY  - JOUR
TI  - The Nanosuspension Formulations of Daidzein:
Preparation and In Vitro Characterization
AB  - Objectives: Daidzein (DZ), a water-insoluble isoflavone, has many beneficial effects (anti-inflammatory, antioxidant, and anticancer effects, etc.)
on human health. DZ has a very low oral bioavailability related to its physicochemical properties (low solubility, intense metabolism of DZ in the
intestine and liver). This study aimed to prepare and in vitro characterize the nanosuspension formulations of DZ to improve the poor solubility and
efficacy of DZ.
Materials and Methods: DZ nanosuspension formulations were prepared with media milling technique using zirconium oxide beads as milling
media. Pluronic F127 and polyvinylpyrrolidone (PVP) K30 (formulation A; F-A) and sodium dodecyl sulfate (SDS) (SDS + pluronic F127 + PVP K30;
formulation B; F-B) were used as stabilizers. The nanosuspension formulations were evaluated for morphological properties, particle sizes, zeta
potential, DZ content, saturation solubility, dissolution, and their cytotoxic effects on RG2 glioblastoma tumor cells.
Results: F-A and F-B formulations were nanosized (in the range of about 181-235 nm) and had negative zeta potential values before and after
lyophilization. The DZ content of F-A and F-B formulations were found to be 93.68±0.78% and 89.75±0.49%, respectively. Fourier transform infrared
spectroscopy analysis showed that there was no significant interaction between DZ and the excipients. Differential scanning calorimetry and X-ray
diffraction analyses confirmed no change in the crystal structure of DZ in F-A and F-B formulations.
Conclusion: In this study, the nanosuspension formulations were successfully prepared and characterized in vitro. Nanosuspension formulations
increased the saturation solubility, dissolution rate, and cytotoxic effect of DZ.
AU  - ÇETİN, Meltem
AU  - OZNULUER OZER, Tuba
AU  - Vural, Imran
AU  - KAPLAN, Afife Büşra UĞUR
AU  - Ozturk, Naile
DO  - 10.4274/tjps.galenos.2021.81905
PY  - 2022
JO  - Turkish Journal of Pharmaceutical Sciences
VL  - 19
IS  - 1
SN  - 1304-530X
SP  - 84
EP  - 92
DB  - TRDizin
UR  - http://search/yayin/detay/525166
ER  -