Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity
Yıl: 2022 Cilt: 14 Sayı: 1 Sayfa Aralığı: 18 - 22 Metin Dili: İngilizce DOI: 10.14744/iksstd.2021.04834 İndeks Tarihi: 26-06-2022
Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity
Öz: Objective: Obesity requires a flexible extracellular matrix (ECM) as it is characterized by adipose tissue expansion. ECM plays an important role in adipocyte development and function, lipid metabolism, and obesity. One of the most important enzymes in the remodeling of ECM is matrix metalloproteinase-9 (MMP-
9). In our study, we aimed to investigate MMP-9 gene expression in adipose tissue in morbidly obese patients and to reveal their relationship with obesity.
Method: This study comprised 44 people, 14 of whom were of normal weight and 30 were morbidly obese. MMP-9 gene expression in adipose tissue was evaluated by quantitative real time reverse transcription polymerase chain reaction (qRT PCR). The data were evaluated by statistical methods.
Results: MMP-9 mRNA levels were found to be slightly higher in obese patients (6.36±2.54) than healthy controls (4.41±1.03); however, this was not statistically
significant (p=0.570). Furthermore, there was no difference in MMP-9 mRNA levels between men and women in both groups (p=0.925).
Conclusion: We showed that the MMP-9 mRNA level in visceral adipose tissue (VAT) was slightly higher in morbidly obese patients than in nonobese controls;
however, the difference was not statistically significant. More research is needed to completely understand the role of MMP-9 in obesity.
Anahtar Kelime: Adipoz Dokuda MMP-9 Gen Ekspresyonu ile Morbid Obezite Arasındaki İlişki
Öz: Amaç: Obezite, yağ dokusu genişlemesi ile karakterize olduğu için esnek bir hücre dışı matriks (HDM) gerektirir. HDM, adiposit gelişimi ve fonksiyonu, lipid
metabolizması ve obezitede önemli bir rol oynar. HDM 'nin yeniden şekillenmesinde en önemli enzimlerden biri matriks metalloproteinaz-9'dur (MMP-9). Çalışmamızda, morbid obez hastaları yağ dokusunda MMP-9 gen ekspresyonu araştırmak ve obezite ile ilişkisini ortaya çıkarmak amaçlanmıştır.
Yöntem: Çalışmaya 14'ü normal kilolu, 30'u morbid obez olmak üzere 44 kişi dahil edilmiştir. Adipoz dokuda MMP-9 gen ekspresyonu, Gerçek Zamanlı Ters
Transkriptaz Polimeraz Zincir Reaksiyonu (qRT PCR) ile değerlendirilmiştir. Veriler istatistiksel yöntemlerle değerlendirilmiştir.
Bulgular: MMP-9 mRNA düzeyleri obez hastalarda (6,36±2,54) sağlıklı kontrollerden (4,41±1,03) biraz daha yüksek bulunmuştur, ancak istatistiksel olarak anlamlı değildir (p=0,570). Ayrıca, her iki grupta da kadın ve erkek arasında MMP-9 mRNA seviyelerinde fark yoktur (p=0.925).
Sonuç: Morbid obez hastaların visseral yağ dokusunda, MMP-9 mRNA seviyesinin obez olmayan kontrollere göre hafifçe yüksek olduğu gösterilmiştir, ancak aralarındaki fark istatistiksel olarak anlamlı bulunmamıştır. MMP-9'un obezitedeki rolünü tam olarak anlamak için daha fazla araştırmaya ihtiyaç vardır.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Koc G, SOYOCAK A, Calis I, Kankaya B, Turgut Cosan D, Alis H (2022). Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. , 18 - 22. 10.14744/iksstd.2021.04834 |
| Chicago | Koc Gulsah,SOYOCAK Ahu,Calis Ibrahim Ugur,Kankaya Burak,Turgut Cosan Didem,Alis Halil Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. (2022): 18 - 22. 10.14744/iksstd.2021.04834 |
| MLA | Koc Gulsah,SOYOCAK Ahu,Calis Ibrahim Ugur,Kankaya Burak,Turgut Cosan Didem,Alis Halil Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. , 2022, ss.18 - 22. 10.14744/iksstd.2021.04834 |
| AMA | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. . 2022; 18 - 22. 10.14744/iksstd.2021.04834 |
| Vancouver | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. . 2022; 18 - 22. 10.14744/iksstd.2021.04834 |
| IEEE | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H "Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity." , ss.18 - 22, 2022. 10.14744/iksstd.2021.04834 |
| ISNAD | Koc, Gulsah vd. "Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity". (2022), 18-22. https://doi.org/10.14744/iksstd.2021.04834 |
| APA | Koc G, SOYOCAK A, Calis I, Kankaya B, Turgut Cosan D, Alis H (2022). Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. İstanbul Kanuni Sultan Süleyman Tıp Dergisi, 14(1), 18 - 22. 10.14744/iksstd.2021.04834 |
| Chicago | Koc Gulsah,SOYOCAK Ahu,Calis Ibrahim Ugur,Kankaya Burak,Turgut Cosan Didem,Alis Halil Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. İstanbul Kanuni Sultan Süleyman Tıp Dergisi 14, no.1 (2022): 18 - 22. 10.14744/iksstd.2021.04834 |
| MLA | Koc Gulsah,SOYOCAK Ahu,Calis Ibrahim Ugur,Kankaya Burak,Turgut Cosan Didem,Alis Halil Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. İstanbul Kanuni Sultan Süleyman Tıp Dergisi, vol.14, no.1, 2022, ss.18 - 22. 10.14744/iksstd.2021.04834 |
| AMA | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. İstanbul Kanuni Sultan Süleyman Tıp Dergisi. 2022; 14(1): 18 - 22. 10.14744/iksstd.2021.04834 |
| Vancouver | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity. İstanbul Kanuni Sultan Süleyman Tıp Dergisi. 2022; 14(1): 18 - 22. 10.14744/iksstd.2021.04834 |
| IEEE | Koc G,SOYOCAK A,Calis I,Kankaya B,Turgut Cosan D,Alis H "Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity." İstanbul Kanuni Sultan Süleyman Tıp Dergisi, 14, ss.18 - 22, 2022. 10.14744/iksstd.2021.04834 |
| ISNAD | Koc, Gulsah vd. "Relationship between MMP-9 Gene Expression in Adipose Tissue and Morbid Obesity". İstanbul Kanuni Sultan Süleyman Tıp Dergisi 14/1 (2022), 18-22. https://doi.org/10.14744/iksstd.2021.04834 |
