Molecular characterization of Bacillus anthracis isolates recovered from nomic and
nonnomic hosts

Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma, soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule (Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as being the predominant type for B. anthracis isolates.

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APA	Akca D, Buyuk F, Karakaya E, Coşkun M, Çelik E, Şahin M, Çelebi Ö, Otlu S, GÜLMEZ SAĞLAM A, BÜYÜK E, DURHAN S, SATICIOGLU I, ABAY S, kayman t, AYDIN F (2022). Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. , 44 - 51. 10.3906/vet-2106-111
Chicago	Akca Dogan,Buyuk Fatih,Karakaya Emre,Coşkun Mustafa Reha,Çelik Elif,Şahin Mitat,Çelebi Özgür,Otlu Salih,GÜLMEZ SAĞLAM Aliye,BÜYÜK Eray,DURHAN SEDA,SATICIOGLU Izzet Burcin,ABAY Seçil,kayman tuba,AYDIN Fuat Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. (2022): 44 - 51. 10.3906/vet-2106-111
MLA	Akca Dogan,Buyuk Fatih,Karakaya Emre,Coşkun Mustafa Reha,Çelik Elif,Şahin Mitat,Çelebi Özgür,Otlu Salih,GÜLMEZ SAĞLAM Aliye,BÜYÜK Eray,DURHAN SEDA,SATICIOGLU Izzet Burcin,ABAY Seçil,kayman tuba,AYDIN Fuat Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. , 2022, ss.44 - 51. 10.3906/vet-2106-111
AMA	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. . 2022; 44 - 51. 10.3906/vet-2106-111
Vancouver	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. . 2022; 44 - 51. 10.3906/vet-2106-111
IEEE	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F "Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts." , ss.44 - 51, 2022. 10.3906/vet-2106-111
ISNAD	Akca, Dogan vd. "Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts". (2022), 44-51. https://doi.org/10.3906/vet-2106-111

APA	Akca D, Buyuk F, Karakaya E, Coşkun M, Çelik E, Şahin M, Çelebi Ö, Otlu S, GÜLMEZ SAĞLAM A, BÜYÜK E, DURHAN S, SATICIOGLU I, ABAY S, kayman t, AYDIN F (2022). Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. Turkish Journal of Veterinary and Animal Sciences, 46(1), 44 - 51. 10.3906/vet-2106-111
Chicago	Akca Dogan,Buyuk Fatih,Karakaya Emre,Coşkun Mustafa Reha,Çelik Elif,Şahin Mitat,Çelebi Özgür,Otlu Salih,GÜLMEZ SAĞLAM Aliye,BÜYÜK Eray,DURHAN SEDA,SATICIOGLU Izzet Burcin,ABAY Seçil,kayman tuba,AYDIN Fuat Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. Turkish Journal of Veterinary and Animal Sciences 46, no.1 (2022): 44 - 51. 10.3906/vet-2106-111
MLA	Akca Dogan,Buyuk Fatih,Karakaya Emre,Coşkun Mustafa Reha,Çelik Elif,Şahin Mitat,Çelebi Özgür,Otlu Salih,GÜLMEZ SAĞLAM Aliye,BÜYÜK Eray,DURHAN SEDA,SATICIOGLU Izzet Burcin,ABAY Seçil,kayman tuba,AYDIN Fuat Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. Turkish Journal of Veterinary and Animal Sciences, vol.46, no.1, 2022, ss.44 - 51. 10.3906/vet-2106-111
AMA	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. Turkish Journal of Veterinary and Animal Sciences. 2022; 46(1): 44 - 51. 10.3906/vet-2106-111
Vancouver	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts. Turkish Journal of Veterinary and Animal Sciences. 2022; 46(1): 44 - 51. 10.3906/vet-2106-111
IEEE	Akca D,Buyuk F,Karakaya E,Coşkun M,Çelik E,Şahin M,Çelebi Ö,Otlu S,GÜLMEZ SAĞLAM A,BÜYÜK E,DURHAN S,SATICIOGLU I,ABAY S,kayman t,AYDIN F "Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts." Turkish Journal of Veterinary and Animal Sciences, 46, ss.44 - 51, 2022. 10.3906/vet-2106-111
ISNAD	Akca, Dogan vd. "Molecular characterization of Bacillus anthracis isolates recovered from nomic and nonnomic hosts". Turkish Journal of Veterinary and Animal Sciences 46/1 (2022), 44-51. https://doi.org/10.3906/vet-2106-111