TY  - JOUR
TI  - Molecular characterization of Bacillus anthracis isolates recovered from nomic and
nonnomic hosts
AB  - Anthrax, which is primarily a disease of herbivores, is known to infect many animal species. In order to break the cycle
of anthrax infection, it is important to identify the potential hosts or reservoirs of its causative agent. Therefore, this study aimed to
characterize Bacillus anthracis isolates recovered from a variety of hosts and environments during anthrax sporadic in the Kars and
Kayseri regions of Turkey. For this purpose, a total of 23 B. anthracis field isolates obtained from human, cattle, sheep, dog, horse, puma,
soil, and fodder were used. In addition, the Sterne vaccine strain was included. All B. anthracis isolates were confirmed via molecular
tests using plasmid-based PCR and 16S rRNA gene sequencing. All were found to be positive for protective antigen (PA) and capsule
(Cap) genes. The presence of two specific 16S rRNA positions (1139 and 1148) with nucleotide mismatches was used to differentiate
between B. anthracis species in the Bacillus cereus group, which has a relatively high (>99%) 16S rRNA sequence similarity. All of our
isolates, including the Sterne vaccine strain, were classified in the same 16S rRNA type (Type 6) that has been reported previously as
being the predominant type for B. anthracis isolates.
AU  - ABAY, Seçil
AU  - AYDIN, Fuat
AU  - DURHAN, SEDA
AU  - Buyuk, Fatih
AU  - GÜLMEZ SAĞLAM, Aliye
AU  - Çelik, Elif
AU  - Coşkun, Mustafa Reha
AU  - Otlu, Salih
AU  - BÜYÜK, Eray
AU  - Şahin, Mitat
AU  - Çelebi, Özgür
AU  - kayman, tuba
AU  - Karakaya, Emre
AU  - SATICIOGLU, Izzet Burcin
AU  - Akca, Dogan
DO  - 10.3906/vet-2106-111
PY  - 2022
JO  - Turkish Journal of Veterinary and Animal Sciences
VL  - 46
IS  - 1
SN  - 1300-0128
SP  - 44
EP  - 51
DB  - TRDizin
UR  - http://search/yayin/detay/526802
ER  -