Laccase bound to cryogel functionalized with phenylalanine for the decolorization of
textile dyes

İdil, Neslihan; Perçin, Işık; ARACAGOK, YUSUF DORUK; Denizli, Adil; Mattiasson, Bo

doi:10.3906/kim-2106-16

Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes

Işık PERÇİN DEMİRÇELİK, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Moleküler Biyoloji Anabilim Dalı, Ankara, Türkiye)

Y. Doruk ARACAGÖK, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Biyoteknoloji Anabilim Dalı, Ankara, Türkiye)

Neslihan İDİL, (Hacettepe Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Biyoteknoloji Anabilim Dalı, Ankara, Türkiye)

Adil DENİZLİ, (Hacettepe Üniversitesi, Fen Fakültesi, Kimya Bölümü, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Bo MATTIASSON (Division of Biotechnology, Lund University, Lund, Sweden)

Turkish Journal of Chemistry

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Yıl: 2021 Cilt: 45 Sayı: 5 Sayfa Aralığı: 1353 - 1365 Metin Dili: İngilizce DOI: 10.3906/kim-2106-16 İndeks Tarihi: 30-06-2022

Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes

Öz:

In this study, amino acid functionalized poly(2-hydroxyethyl methacrylate-N-methacrylolyl-l-phenylalanine) [PHEMAPA] cryogel discs were prepared. In this respect, phenylalanine containing N-methacryloyl-(L)-phenylalanine methyl ester (MAPA) was polymerized with 2-hydroxyethyl methacrylate (HEMA) without requirement of any activation step. Laccase bound poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-phenylalanine) [Lac-PHEMAPA] cryogel discs were applied for decolorization of Reactive Blue-247 (RB-247). The ability of Lac-PHEMAPA cryogel discs on dye decolorization was found to be as 90% in 2 h and even more within 4h. The decolorization activities of 86% and 73% were observed at relatively low (4°C) and high (60°C) temperatures, respectively. The effect of dye concentration on dye decolorization and 100% decolorization activity was achieved in dye concentration between 50–300 ppm. Lac-PHEMAPA cryogel discs maintained 80% of its decolorization activity after six cycles. Consequently, the PHEMAPA cryogel discs are promising materials for immobilizing laccase. The Lac-PHEMAPA has a rapid dye decolorization in a broad range of temperature. The preparation is furthermore very stable and activity is preserved during storage.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Pramanik S, Chaudhuri S. Laccase activity and azo dye decolorization potential of podoscypha elegans. Mycobiology 2018; (46): 79–83. doi: 10.1080/12298093.2018.1454006
2. Pandey A, Singh P, Iyengar L. Bacterial decolorization and degradation of azo dyes. International Biodeterioration and Biodegradation 2007; (59): 73–84. doi: 10.1016/j.ibiod.2006.08.006
3. Akkaya A, Erdogan Ozseker E, Akdogan HA. Degradation of Dyes by Laccase. Analytical Letters 2016; (49): 790–798. doi: 10.1080/00032719.2015.1081916
4. Brijwani K, Rigdon A, Vadlani PV. Fungal laccases: Production, function, and applications in food processing. Enzyme Research 2010; Article ID 149748. doi: 10.4061/2010/149748
5. Mendoza L, Jonstrup M, Hatti-Kaul R, Mattiasson B. Azo dye decolorization by a laccase/mediator system in a membrane reactor: Enzyme and mediator reusability. Enzyme and Microbial Technology 2011; (49): 478-484. doi: 10.1016/j.enzmictec.2011.08.006
6. Forootanfar H, Moezzi A, Aghaie-Khouzani M, Janlou Y, Niknejad F et al. Synthetic dye decolorization by three sources of fungal laccase. Research journal of chemistry and environment 2013; (9): 27.
7. Zhang L, Lu H, Yu J, McSporran E, Khan A et al. Preparation of high-strength sustainable lignocellulose gels and their applications for antiultraviolet weathering and dye removal. ACS Sustainable Chemistry & Engineering 2019; (7): 2998-3009. doi: 10.1021/ acssuschemeng.8b04413
8. Chethana M, Sorokhaibam LG, Bhandari M, Raja S, Ranade VV. Green Approach to Dye Wastewater Treatment Using Biocoagulants. ACS Sustainable Chemistry & Engineering 2016; (4): 2495−2507. doi: 10.1021/acssuschemeng.5b01553
9. Punzi M, Nilsson F, Anbalagen A, Svensson B-M, Jonstrup M et al. Combined anaerobic-ozonation process for treatment of textile wastewater: removal of acute toxicity and mutagenicity. Journal of Hazardous Materials 2016; (292): 52-60. doi: 10.1016/j. jhazmat.2015.03.018
10. Qin Y, Wang L, Zhao C, Chen D, Ma Y et al. Ammonium-functionalized hollow polymer particles as a ph-responsive adsorbent for selective removal of acid dye. ACS Applied Materials & Interfaces 2016; (8): 16690-16698.
11. Ramalingam B, Khan MMR, Mondal B, Mandal AB, Das SK. Facile Synthesis of Silver Nanoparticles Decorated Magnetic-Chitosan Microsphere for Efficient Removal of Dyes and Microbial Contaminants. ACS Sustainable Chemistry & Engineering 2015; (3): 2291– 2302. doi: 10.1021/acssuschemeng.5b00577
12. Liu L, Gao ZY, Su XP, Chen X, Jiang L et al. Adsorption Removal of Dyes from Single and Binary Solutions Using a Cellulose-based Bioadsorbent. ACS Sustainable Chemistry & Engineering 2015; (3): 432–442. doi: 10.1021/sc500848m
13. Asliyüce S, Bereli N, Uzun L, Onur MA, Say R et al. Ion-imprinted supermacroporous cryogel, for in vitro removal of iron out of human plasma with beta thalassemia. Separation and Purification Technology 2010; (73): 243-249. doi: 10.1016/j.seppur.2010.04.007
14. Bakhshpour M, Idil N, Perçin I, Denizli A. Biomedical applications of polymeric cryogels Applied Sciences 2019; (9): 553.
15. Tamahkar E, Bakhshpour M, Andaç M, Denizli A. Ion imprinted cryogels for selective removal of Ni(II) ions from aqueous solutions. Separation and Purification Technology 2017; (179): 36-44. doi: 10.1016/j.seppur.2016.12.048
16. Jalilzadeh M, Uzun L, Şenel S, Denizli A. Specific heavy metal ion recovery with ion-imprinted cryogels. Journal of Applied Polymer Science 2016; (133): 43095. doi: 10.1002/app.43095.
17. Tekin K, Uzun L, Şahin ÇA, Bektaş S, Denizli A. Preparation and characterization of composite cryogels containing imidazole group and use in heavy metal removal. Reactive and Functional Polymers 2011; (71): 985-993. doi: 10.1016/j.reactfunctpolym.2011.06.005
18. Lozinsky VI, Plieva FM, Galaev IY, Mattiasson B. The potential of polymeric cryogels in bioseparation. 2001; (10): 163-88. doi: 10.1023/A:1016386902611
19. Lozinsky VI, Galaev IY, Plieva FM, Savina IN, Jungvid H et al. Polymeric cryogels as promising materials of biotechnological interest. Trends in Biotechnology 2003; (21): 445–451. doi: 10.1016/j.tibtech.2003.08.002
20. Göktürk I, Perçin I, Denizli A. Catalase purification from rat liver with iron-chelated poly(hydroxyethyl methacrylate-N-methacryloyl- (l)-glutamic acid) cryogel discs. Preparative Biochemistry & Biotechnology 2016; (46): 602-609. doi: 10.1080/10826068.2015.1085400
21. Erol K, Cebeci BK, Köse K, Köse DA. Effect of immobilization on the activity of catalase carried by poly(HEMA-GMA) cryogels. International Journal of Biological Macromolecules 2019; (123): 738-743. doi: 10.1016/j.ijbiomac.2018.11.121
22. Soomro R, Perçin I, Memon N, Iqbal Bhanger M, Denizli A. Gelatin-loaded p(HEMA-GMA) cryogel for high-capacity immobilization of horseradish peroxidase. Artificial Cells, Nanomedicine, and Biotechnology 2016; (44): 1708-1713. doi: 10.3109/21691401.2015.1089252
23. Öncel Ş, Uzun L, Garipcan B, Denizli A. Synthesis of phenylalanine-containing hydrophobic beads for lysozyme adsorption. Industrial & Engineering Chemistry Research 2005; (44): 7049-7056. doi: 10.1021/ie0506318
24. Öztürk N, Akgöl S, Arisoy M, Denizli A. Reversible adsorption of lipase on novel hydrophobic nanospheres.. Separation and Purification Technology 2007; (58): 83-90. doi: 10.1016/j.seppur.2007.07.037
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 1951; (193):256-275. doi: 10.1016/S0021-9258(19)52451-6
26. Yaneva S, Rangelova N, Aleksandrov L, Danalev D. Enzymatic decolorization of dyes by laccase immobilized on hybrid carriers. Journal of Chemical Technology and Metallurgy 2018; (53): 1203-1210. doi: 10.1002/2015TC003941
27. Lu L, Zhao M, Wang Y. Immobilization of laccase by alginate-chitosan microcapsules and its use in dye decolorization. World Journal of Microbiology and Biotechnology 2007; (23): 159–166. doi: 10.1007/s11274-006-9205-6
28. Daâssi D, Rodríguez-Couto S, Nasri M, Mechichi T. Biodegradation of textile dyes by immobilized laccase from Coriolopsis gallica into Ca-alginate beads. International Biodeterioration and Biodegradation 2014; (90): 71-78. doi: 10.1016/j.ibiod.2014.02.006
29. Peralta-Zamora P, Pereira CM, Tiburtius ERL, Moraes SG, Rosa MA et al. Decolorization of reactive dyes by immobilized laccase. Applied Catalysis B: Environmental 2003; (42): 131-144. doi: 10.1016/S0926-3373(02)00220-5
30. Mogharabi M, Nassiri-Koopaei N, Bozorgi-Koushalshahi M, Nafissi-Varcheh N, Bagherzadeh G et al. Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes Bioinorganic Chemistry and Applications. 2012; Article ID 823830. doi: 10.1155/2012/82383
31. Roy R, Fakhruddin A, Khatun R, Islam M, Ahsan M et al. Characterization of Textile Industrial Effluents and its Effects on Aquatic Macrophytes and Algae. Bangladesh Journal of Scientific and Industrial Research 2010; (45): 79-84. doi: 10.3329/bjsir.v45i1.5187
32. Khan H, Ahmad N, Yasar A, Shahid R. Advanced oxidative decolorization of red c1-5b: Effects of dye concentration, process optimization and reaction kinetics. Polish Journal of Environmental Studies 2010; (19): 83-92.
33. Froehner SC, Eriksson, KE, Rosenblom J, Hörnfeldt AB, Servin R et al. Properties of the glycoprotein laccase immobilized by two methods. Acta Chemica Scandinavica 2008; (29): 691. doi: 10.3891/acta.chem.scand.29b-0691
34. Saoudi O, Ghaouar N, Ben Salah S, Othman T. Denaturation process of laccase in various media by refractive index measurements. Biochemistry and Biophysics Reports 2017; (11): 19–26. doi: 10.1016/j.bbrep.2017.05.005
35. Wu MH, Lee CC, Hsiao AS, Yu SM, Wang AHJ et al. Kinetic analysis and structural studies of a high-efficiency laccase from Cerrena sp. RSD1.FEBS Open Bio 2018; (8): 1230-1246
36. LeVan MD, Vermeulen T. Binary Langmuir and Freundlich isotherms for ideal adsorbed solutions. The Journal of Physical Chemistry 1981; (85): 3247–3250. doi: 10.1021/j150622a009
37. Cheung CW, Porter JF, Mckay G. Sorption kinetic analysis for the removal of cadmium ions from effluents using bone char. Water Research 2001; (35): 605–612. doi: 10.1016/S0043-1354(00)00306-7
38. Šekuljica N, Prlainović N, Stefanović AB, Žuža MG, Čičkarić DZ et al. Decolorization of anthraquinonic dyes from textile effluent using horseradish peroxidase: Optimization and kinetic study The Scientific World Journal 2015; Article ID 371625. doi: 10.1155/2015/371625
39. Lavanya C, Dhankar R, Chhikara S, Sheoran S. Review Article Degradation of Toxic Dyes : A Review. International Journal of Current Microbiology and Applied Sciences 2014; (3): 189-199.
40. Gao Y, Yang B, Wang Q. Biodegradation and Decolorization of Dye Wastewater: A Review. In Proceedings of the IOP Conference Series: Earth and Environmental Science 2018. doi: 10.1088/1755-1315/178/1/012013
41. Zille A, Tzanov T, Gübitz GM, Cavaco-Paulo A. Immobilized laccase for decolourization of reactive Black 5 dyeing effluent. Biotechnology Letters 2003; (25): 1473–1477. doi: 10.1023/A:1025032323517
42. Champagne PP, Ramsay JA. Dye decolorization and detoxification by laccase immobilized on porous glass beads. Bioresource Technology 2010; (101): 2230-2235. doi: 10.1016/j.biortech.2009.11.066
43. Cristóvão RO, Tavares APM , Brígida AI, Loureiro JM, Boaventura RAR et al. Immobilization of commercial laccase onto green coconut fiber by adsorption and its application for reactive textile dyes degradation. Journal of Molecular Catalysis B: Enzymatic 2011; (72): 6-12.
44. Chhabra M, Mishra S, Sreekrishnan TR, Immobilized laccase mediated dye decolorization and transformation pathway of azo dye acid red 27. Journal of Environmental Health Science & Engineering 2015; (13): 1–9. doi: 10.1186/s40201-015-0192-0
45. Kandelbauer A, Maute O, Kessler RW, Erlacher A, Gübitz GM. Study of dye decolorization in an immobilized laccase enzyme-reactor using online spectroscopy. Biotechnology and Bioengineering 2004; (87): 552-63. doi: 10.1002/bit.20162
46. Ilk S, Demircan D, Sağlam S, Sağlam N, Rzayev ZMO. Immobilization of laccase onto a porous nanocomposite: Application for textile dye degradation. Turkish Journal of Chemistry 2016; (40): 262–276. doi: 10.3906/kim-1504-63
47. Li W-X, Sun H-Y, Zhang R-F. Global Conference on Polymer and Composite Materials (PCM 2015) IOP Publishing, IOP Conf. Series: Materials Science and Engineering 2015; 87: 012033.
48. Ping W, Xuerong F, Li C, Qiang W, Aihui Z. Decolorization of reactive dyes by laccase immobilized in alginate/gelatin blent with PEG. Journal of Environmental Sciences 2008; (20): 1519-1522. doi: 10.1016/S1001-0742(08)62559-0
49. Abadulla E, Tzanov T, Costa S, Robra KH, Cavaco-Paulo A et al. Decolorization and detoxification of textile dyes with a laccase from Trametes hirsuta. Applied and Environmental Microbiology 2000; (66): 3357–3362. doi: 10.1128/AEM.66.8.3357-3362.2000
50. Uygun M. Preparation of laccase immobilized cryogels and usage for decolorization. Journal of Chemistry 2013; Article ID 387181. doi: 10.1155/2013/387181
51. Stoilova I, Krastanov A, Stanchev V. Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation. Advances in Bioscience and Biotechnology 2010; (01): 208–215. doi: 10.4236/abb.2010.13029
52. Ratanapongleka K, Phetsom J. Decolorization of Synthetic Dyes by Crude Laccase from Lentinus Polychrous Lev. International Journal of Chemical Engineering and Applications 2014; (5): 26–30. doi: 10.7763/IJCEA.2014.V5.345
53. Plieva FM, Mattiasson B. Macroporous gel particles as novel sorbent materials:Rational design. Industrial & Engineering Chemistry Research 2008; (5047): 4131-4141. doi: 10.1021/ie071406o
54. Jonstrup M, Wärjerstam M, Murto M, Mattiasson B. Immobilization of TiO2 for combined photocatalytic-biological azo dye degradation. Water Science and Tehnology 2010; (62): 525-531.
55. Alinsafi A, Evenou F, Abdulkarim EM, Pon MN, Zahraa O et al. Treatment of textile industry watewaterby supported photocatalysis. Dyes and Pigment 2007; (74): 439-345.

APA	Perçin I, ARACAGOK Y, İdil N, Denizli A, Mattiasson B (2021). Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. , 1353 - 1365. 10.3906/kim-2106-16
Chicago	Perçin Işık,ARACAGOK YUSUF DORUK,İdil Neslihan,Denizli Adil,Mattiasson Bo Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. (2021): 1353 - 1365. 10.3906/kim-2106-16
MLA	Perçin Işık,ARACAGOK YUSUF DORUK,İdil Neslihan,Denizli Adil,Mattiasson Bo Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. , 2021, ss.1353 - 1365. 10.3906/kim-2106-16
AMA	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. . 2021; 1353 - 1365. 10.3906/kim-2106-16
Vancouver	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. . 2021; 1353 - 1365. 10.3906/kim-2106-16
IEEE	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B "Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes." , ss.1353 - 1365, 2021. 10.3906/kim-2106-16
ISNAD	Perçin, Işık vd. "Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes". (2021), 1353-1365. https://doi.org/10.3906/kim-2106-16

APA	Perçin I, ARACAGOK Y, İdil N, Denizli A, Mattiasson B (2021). Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. Turkish Journal of Chemistry, 45(5), 1353 - 1365. 10.3906/kim-2106-16
Chicago	Perçin Işık,ARACAGOK YUSUF DORUK,İdil Neslihan,Denizli Adil,Mattiasson Bo Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. Turkish Journal of Chemistry 45, no.5 (2021): 1353 - 1365. 10.3906/kim-2106-16
MLA	Perçin Işık,ARACAGOK YUSUF DORUK,İdil Neslihan,Denizli Adil,Mattiasson Bo Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. Turkish Journal of Chemistry, vol.45, no.5, 2021, ss.1353 - 1365. 10.3906/kim-2106-16
AMA	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. Turkish Journal of Chemistry. 2021; 45(5): 1353 - 1365. 10.3906/kim-2106-16
Vancouver	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes. Turkish Journal of Chemistry. 2021; 45(5): 1353 - 1365. 10.3906/kim-2106-16
IEEE	Perçin I,ARACAGOK Y,İdil N,Denizli A,Mattiasson B "Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes." Turkish Journal of Chemistry, 45, ss.1353 - 1365, 2021. 10.3906/kim-2106-16
ISNAD	Perçin, Işık vd. "Laccase bound to cryogel functionalized with phenylalanine for the decolorization of textile dyes". Turkish Journal of Chemistry 45/5 (2021), 1353-1365. https://doi.org/10.3906/kim-2106-16