Yıl: 2021 Cilt: 45 Sayı: 5 Sayfa Aralığı: 1621 - 1638 Metin Dili: İngilizce DOI: 10.3906/kim-2104-17 İndeks Tarihi: 30-06-2022

Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS

Öz:
The study aims to determine the secondary metabolites of Hypericum androsaemum L. extracts by liquid chromatographyhigh resolution mass spectrometry (LC-HRMS), and investigate the antioxidant and cytotoxic activities of the plant. Cytotoxic activity was evaluated by MTT assay, and apoptosis induction abilities on human prostate adenocarcinoma (PC-3), and hepatocellular carcinoma (Hep G2) cell lines. Accordingly, major secondary metabolites were found as hederagenin (762 ± 70.10 μg/g) in the leaves dichloromethane (LD), herniarin (167 ± 1.50 μg/g) in fruit dichloromethane (FD), (-)-epicatechin (6538 ± 235.36 μg/g) in the leaves methanol (LM), (-)-epigallocatechin gallate (758 ± 20.46 μg/g) in the fruit methanol (FM), and caffeic acid (370 ± 8.88 μg/g) in the fruit water (FW), and (3313 ± 79.51 μg/g) in the leaves water (LW) extracts. LM exerted strong antioxidant activity in DPPH free (IC50 10.94 ± 0.08 μg/mL), and ABTS cation radicals scavenging (IC50 9.09 ± 0.05 μg/mL) activities. FM exhibited cytotoxic activity with IC50 values of 73.23 ± 3.06 µg/mL and 31.64 ± 2.75 µg/mL on PC-3 and Hep G2 cell lines, respectively. Being the richest extract in terms of quillaic acid (630 ± 18.9 μg/g), which is a well-known cytotoxic triterpenoid with proven apoptosis induction ability on different cells, FM extract showed apoptosis induction activity with 64.75% on PC-3 cells at 50 μg/mL concentration. The study provides promising results about the potential of Hypericum androsaemum on cancer prevention.
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APA YAZICI BEKTAŞ N, Ersoy E, Boga M, boran t, çınar e, Ozhan G, Goren A, EROGLU OZKAN E (2021). Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. , 1621 - 1638. 10.3906/kim-2104-17
Chicago YAZICI BEKTAŞ NURDAN,Ersoy Ezgi,Boga Mehmet,boran tugce,çınar ercan,Ozhan Gul,Goren Ahmet C,EROGLU OZKAN ESRA Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. (2021): 1621 - 1638. 10.3906/kim-2104-17
MLA YAZICI BEKTAŞ NURDAN,Ersoy Ezgi,Boga Mehmet,boran tugce,çınar ercan,Ozhan Gul,Goren Ahmet C,EROGLU OZKAN ESRA Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. , 2021, ss.1621 - 1638. 10.3906/kim-2104-17
AMA YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. . 2021; 1621 - 1638. 10.3906/kim-2104-17
Vancouver YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. . 2021; 1621 - 1638. 10.3906/kim-2104-17
IEEE YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E "Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS." , ss.1621 - 1638, 2021. 10.3906/kim-2104-17
ISNAD YAZICI BEKTAŞ, NURDAN vd. "Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS". (2021), 1621-1638. https://doi.org/10.3906/kim-2104-17
APA YAZICI BEKTAŞ N, Ersoy E, Boga M, boran t, çınar e, Ozhan G, Goren A, EROGLU OZKAN E (2021). Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry, 45(5), 1621 - 1638. 10.3906/kim-2104-17
Chicago YAZICI BEKTAŞ NURDAN,Ersoy Ezgi,Boga Mehmet,boran tugce,çınar ercan,Ozhan Gul,Goren Ahmet C,EROGLU OZKAN ESRA Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry 45, no.5 (2021): 1621 - 1638. 10.3906/kim-2104-17
MLA YAZICI BEKTAŞ NURDAN,Ersoy Ezgi,Boga Mehmet,boran tugce,çınar ercan,Ozhan Gul,Goren Ahmet C,EROGLU OZKAN ESRA Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry, vol.45, no.5, 2021, ss.1621 - 1638. 10.3906/kim-2104-17
AMA YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry. 2021; 45(5): 1621 - 1638. 10.3906/kim-2104-17
Vancouver YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS. Turkish Journal of Chemistry. 2021; 45(5): 1621 - 1638. 10.3906/kim-2104-17
IEEE YAZICI BEKTAŞ N,Ersoy E,Boga M,boran t,çınar e,Ozhan G,Goren A,EROGLU OZKAN E "Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS." Turkish Journal of Chemistry, 45, ss.1621 - 1638, 2021. 10.3906/kim-2104-17
ISNAD YAZICI BEKTAŞ, NURDAN vd. "Cytotoxic and apoptotic effects of Hypericum androsaemum on prostate adenocarcinoma (PC-3) and hepatocellular carcinoma (Hep G2) cell lines with identification of secondary metabolites by LC-HRMS". Turkish Journal of Chemistry 45/5 (2021), 1621-1638. https://doi.org/10.3906/kim-2104-17