TY  - JOUR
TI  - Effects of dietary calcium, phosphorus and microbial phytase on intestinal morphology
in laying hens
AB  - Different challenges are being applied in the poultry industry in order to protect animal health and to increase immunity and
production. The supplementation of microbial phytase is essential in terms of both reducing the inorganic phytase rate and contributing
to the absorption of other minerals. In this study, a newly isolated microbial phytase was added at different concentrations to the diet
together with calcium (Ca2+) and available phosphorus (AP), and the effects of this supplementation on intestinal absorption capacity
and Ca2+ binding capacity were investigated via morphological measurements and immunohistochemical examination of the duodenum
and ileum. For this purpose, 90 Lohmann LSL-White laying hens were divided into three main diet groups: 1. Standard Ca2+ and AP
(Ca+AP), 2. Standard Ca2+ and low AP (Ca+low AP), and 3. Low Ca2+ and low AP (low Ca+low AP). These three groups were further
divided into three phytase subgroups each (without phytase [Phy-], commercial phytase [CP] and microbial phytase [MP]). At the end
of the experiment, animals were euthanized, and duodenum and ileum samples were fixed and processed for histological examination.
Villus height, crypt depth, total mucosa thickness, and villus width were measured and villus height: crypt depth ratio and villus
absorption area were calculated. Caldesmon expression in the duodenum and ileum was also investigated immunohistochemically. The
results indicated that villus height, total mucosa thickness, and villus absorption area increased (p ≤ 0.05) in birds fed with Ca+AP+MP.
Stronger caldesmon expression was observed in the MP treated groups. We concluded that MP produced from Bacillus megaterium
EBD 9-1 bacterium increases the utilization of Ca2+ and AP and, thus, can have a beneficial role when these macrominerals are used
insufficiently. Ca2+, AP, and MP may have positive effects on the intestinal morphology and absorption area when used at optimum
amounts.
AU  - Gültepe, Eyüp Eren
AU  - işbilir, ihsan
AU  - guler, sabire
AU  - CENGIZ, SERIFE SULE
AU  - VAROL KAYAPUNAR, Nuray
AU  - Şanlı, Batuhan
AU  - ASMAZ, Ender Deniz
AU  - Yesilbag, Derya
DO  - 10.3906/vet-2107-73
PY  - 2022
JO  - Turkish Journal of Veterinary and Animal Sciences
VL  - 46
IS  - 2
SN  - 1300-0128
SP  - 293
EP  - 303
DB  - TRDizin
UR  - http://search/yayin/detay/527860
ER  -