Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations
with milk yield and milk content traits in Karakaş and Norduz sheep breeds

KARAKUŞ, Kadir; YESILOVA, ABDULLAH; Koyun, Hasan; KIRAZ, SELAHADDIN; YILMAZ, AYHAN; Koncagül, Seyrani; Karaca, Serhat; AYGÜN, TURGUT

doi:10.3906/vet-2010-26

Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds

Hasan KOYUN, (Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Van, Türkiye)

Selahaddin KİRAZ, (Harran Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Şanlıurfa, Türkiye)

Serhat KARACA, (Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Van, Türkiye)

Seyrani KONCAGÜL, (Ankara Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Ankara, Türkiye)

Ayhan YILMAZ, (Siirt Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Siirt, Türkiye)

Kadir KARAKUŞ, (Malatya Turgut Özal Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Malatya, Türkiye)

Abdullah YEŞİLOVA, (Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Van, Türkiye)

Turgut AYGÜN (Van Yüzüncü Yıl Üniversitesi, Ziraat Fakültesi, Hayvan Bilimleri Bölümü, Van, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

2 0

Yıl: 2021 Cilt: 45 Sayı: 5 Sayfa Aralığı: 881 - 889 Metin Dili: İngilizce DOI: 10.3906/vet-2010-26 İndeks Tarihi: 30-06-2022

Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds

Öz:

Karakaş and Norduz sheep have been adapted to the Lake Van region of Turkey for many years as being domestic genetic resources and meat, milk, and fleece traits are a reliable economic resource especially preferred by small family businesses around the area. In this study, the data set consisted of milk yield (MY) and milk content components; milk fat (MF), fat-free dry matter (FFDM), dry matter (DM), protein (Pro), lactose (Lac), pH, acidity H (aH), and lactic acid (Lac). Besides, Karakaş (n = 30) and Norduz (n = 26) sheep were chosen to investigate based on the SNP method. Correspondingly, genomic DNA from both breeds exon 2 of the GDF9 gene region was amplified, 815 base pairs (bp) in length, by means of PCR. Therefore, there were three noval SNPs detected in both breeds under investigation. Although SNP1 and 2 with genotypes of GG and AG had statistically significant impacts on both milk production and milk components (p < 0.01), SNP3 with genotypes of TT, TC had no significant effects on the milk characteristics in question.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Galal H. Biodiversity in goats. Small Ruminant Research 2005; 60 (1): 75-81. doi: 10.1016/j.smallrumres.2005.06.021
2. Yılmaz O, Wilson RT, Kor A, Cengiz F, Ertuğrul M. The farm animal genetic resources of Turkey: sheep – I – common and rare breeds. Bitlis Eren University Journal of Science and Technology 2012; 2 (1): 1-5. doi: 10.17678/beuscitech.47143
3. Aygün T, Bingöl M. Akkaraman varyetesi Karakaş ve Norduzların doğum ağırlıkları bakımından karşılaştırılması. In: Ege Üniversitesi Ziraat Fakültesi Uluslararası Hayvancılık Kongresi; İzmir, Tutkey; 1999. pp. 738-742 (in Turkish).
4. Aygün T, Bingöl M, Gökdal Ö. Comparison of feedlot performance and carcass traits of tail docked Karakas and Norduz male lambs. Indian Veterinary Journal 2006; 83: 869- 872.
5. Bingöl M. Norduz koyunlarının döl ve süt verimi ile büyümegelişme ve dış yapı özellikleri. PhD, Van Yüzüncü Yıl University, Van, Turkey, 1998 (in Turkish).
6. Heaton MP, Harhay GP, Bennett GL, Stone RT, Grosse WM et al. Selection and use of SNP markers for animal identification and paternity analysis in U.S. beef cattle. Mammalian Genome 2002; 13 (5): 272-281. doi: 10.1007/s00335-001-2146-3
7. Weber JL, May PE. Abundunt class of human DNA polymorphism which can be typed using the polymerase chain reaction. American Journal of Human Genetics 1989; 44 (3): 388-396.
8. Dekkers JCM. Application of genomics tools to animal breeding. Current Genomics 2012; 13 (3): 207-212. doi: 10.2174/138920212800543057
9. Araby IEE, Awad A, Saleh AA, Magdy SM. Polymorphisms of growth differentiation factor 9 (GDF 9) and bone morphogenetic protein 15 (BMP15) genes in Barki and Rahmani sheep breeds. Zazazig Veterinary Journal 2019; 47 (2): 160-167. doi: 10.21608/ZVJZ.2019.10071.1025
10. Herpin A, Lelong C, Favrel P. Transforming growth factorbeta-related proteins: an ancestral and widespread superfamily of cytokines in metazoans. Developmental and Comparative Immunology 2004; 28 (5): 461-485. doi: 10.1016/j. dci.2003.09.007
11. Wang X, Yang Q, Wang K, Zhang S, Pan C et al. A novel 12- bp indel polymorphism within the GDF9 gene is significantly associated with litter size and growth traits in goats. Animal Genetics 2017; 48 (6): 720-736. doi: 10.1111/age.12617
12. De Castro F, Cruz MH, Leal CL (2016). Role of growth differentiation factor 9 and bone morphogenetic protein 15 in ovarian function and their importance in mammalian female fertility. Asian-Australasian Journal of Animal Sciences 2016; 29 (8): 1065-1074. doi: 10.5713/ajas.15.0797
13. Hanrahan J, Gregan SM, Mulsant P, Mullen M, Davis GH et al. Mutations in the genes for oocyte-derived growth factors GDF9 and BMP15 are associated with both increased ovulation rate and sterility in Cambridge and Belclare sheep (Ovisaries). Biology of Reproductional 2004; 70 (4): 900-909. doi: 10.1095/ biolreprod.103.023093
14. Sadighi M, Bodensteiner KJ, Beattie AE, Galloway SM. Genetic mapping of ovine growth differentiation factor 9 (GDF9) to sheep chromosome 5. Anim Genetics 2002; 33 (3): 244-245. doi: 10.1046/j.1365-2052.2002.t01-11-00876.x
15. Ghaderi A, Nasiri MTB, Mirzadeh KH, Fayazi J, Sadr AS. Identification of the GDF9 mutation in two sheep breeds by using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) technique. African Journal of Biotechnology 2010; 47: 8020-8022. doi: 10.5897/AJB10.532
16. Dolatabady MM, Habibizad J. Single nucleotide polymorphisms (SNPs) of GDF9 gene in Bahmaei and Lak Ghashghaei sheep breeds and its association with litter size. Iranian Journal of Applied Animal Sciences 2019; 9: 427-432.
17. Kurt A, Çakmakçı S, Çağlar A. Süt ve mamülleri muayene ve analiz metodları rehberi. Atatürk Üniversitesi Yayınları No: 252/D. Erzurum, Turkey: Atatürk Üniversitesi Yayınları; 1996. p. 238 (in Turkish).
18. Ocak E, Bingöl M, Gökdal O. Van yöresinde yetiştirilen Norduz koyunlarının süt bileşimi ve süt verim özellikleri. YYÜ Tarım Bilimleri Dergisi 2009; 19 (2): 85-89 (in Turkish).
19. Maddox JF, Davies KP, CrawfordAM, Hulme DJ, Vaiman D et al. An enhanced linkage map of the sheep genome comprising more than 1000 loci. Genome Research 2001; 11: 1275- 1289. doi: 10.1101/gr.135001
20. Nicol L, Bishop SC, Pong-Wong R, Bendixen C, Holm LA et al. Homozygosity for a single base-pair mutation in the oocytespecific GDF9 gene results in sterility in Thoka sheep. Reproduction 2009; 138 (6): 921-933. doi: 10.1530/REP-09- 0193
21. Liandris E, Kominakis A, Andreadoua M, Kapeoldassi K, Chadioa S et al. Associations between single nucleotide polymorphisms of GDF9 and BMP15 genes and litter size in two dairy sheep breeds of Greece. Small Rumunant Research 2012; 107 (1): 16-21. doi: 10.1016/j.smallrumres.2012.04.004
22. Bahrami Y, Bahrami S, Mohammadi HR, Chekani-Azar V, Mousavizadeh SA. The polymorphism of GDF-9 gene in Hisari sheep. Biological Forum – An International Journal 2014; 6 (2): 46-52.
23. Khodabakhshzadeh R, Mohammadabadi MR, Esmailizadeh AK, Shahrebabak HM, Bordbar F. Identification of point mutations in exon 2 of GDF9 gene in Kermani sheep. Polish Jornal of Veterinary Sciences 2016; 19 (2): 281-289. doi: 10.1515/pjvs-2016-0035
24. El Fiky ZA, Hassan GM, Mohamed I, Nassar MI (2017). Genetic polymorphism of growth differentiation factor 9 (GDF9) gene related to fecundity in two Egyptian sheep breeds. Journal of Assisted Reproduction and Genetics 2017; 34: 1683-1690. doi: 10.1007/s10815-017-1007-2
25. Jawasreh KI, Ismail ZB. Polymorphism of prolactin, growth differentiation factor 9, and calpastatin genes and their effects on weight traits in Awassi lambs. Journal of Advanced Veterinary and Animal Research 2019; 6 (1): 86-91. doi: 10. 5455/javar. 2019. f317
26. Al-Khuzai FLJ, Ahmed JR. Polymorphism of gdf9 (exon-1) gene and its association with milk production and prolificacy of Awassi sheep. Plant Archives 2019; 19 (2): 4037-4040.

APA	Koyun H, KIRAZ S, Karaca S, Koncagül S, YILMAZ A, KARAKUŞ K, YESILOVA A, AYGÜN T (2021). Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. , 881 - 889. 10.3906/vet-2010-26
Chicago	Koyun Hasan,KIRAZ SELAHADDIN,Karaca Serhat,Koncagül Seyrani,YILMAZ AYHAN,KARAKUŞ Kadir,YESILOVA ABDULLAH,AYGÜN TURGUT Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. (2021): 881 - 889. 10.3906/vet-2010-26
MLA	Koyun Hasan,KIRAZ SELAHADDIN,Karaca Serhat,Koncagül Seyrani,YILMAZ AYHAN,KARAKUŞ Kadir,YESILOVA ABDULLAH,AYGÜN TURGUT Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. , 2021, ss.881 - 889. 10.3906/vet-2010-26
AMA	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. . 2021; 881 - 889. 10.3906/vet-2010-26
Vancouver	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. . 2021; 881 - 889. 10.3906/vet-2010-26
IEEE	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T "Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds." , ss.881 - 889, 2021. 10.3906/vet-2010-26
ISNAD	Koyun, Hasan vd. "Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds". (2021), 881-889. https://doi.org/10.3906/vet-2010-26

APA	Koyun H, KIRAZ S, Karaca S, Koncagül S, YILMAZ A, KARAKUŞ K, YESILOVA A, AYGÜN T (2021). Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. Turkish Journal of Veterinary and Animal Sciences, 45(5), 881 - 889. 10.3906/vet-2010-26
Chicago	Koyun Hasan,KIRAZ SELAHADDIN,Karaca Serhat,Koncagül Seyrani,YILMAZ AYHAN,KARAKUŞ Kadir,YESILOVA ABDULLAH,AYGÜN TURGUT Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. Turkish Journal of Veterinary and Animal Sciences 45, no.5 (2021): 881 - 889. 10.3906/vet-2010-26
MLA	Koyun Hasan,KIRAZ SELAHADDIN,Karaca Serhat,Koncagül Seyrani,YILMAZ AYHAN,KARAKUŞ Kadir,YESILOVA ABDULLAH,AYGÜN TURGUT Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. Turkish Journal of Veterinary and Animal Sciences, vol.45, no.5, 2021, ss.881 - 889. 10.3906/vet-2010-26
AMA	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(5): 881 - 889. 10.3906/vet-2010-26
Vancouver	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds. Turkish Journal of Veterinary and Animal Sciences. 2021; 45(5): 881 - 889. 10.3906/vet-2010-26
IEEE	Koyun H,KIRAZ S,Karaca S,Koncagül S,YILMAZ A,KARAKUŞ K,YESILOVA A,AYGÜN T "Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds." Turkish Journal of Veterinary and Animal Sciences, 45, ss.881 - 889, 2021. 10.3906/vet-2010-26
ISNAD	Koyun, Hasan vd. "Single nucleotide polymorphisms of GDF9 gene/exon 2 region and their associations with milk yield and milk content traits in Karakaş and Norduz sheep breeds". Turkish Journal of Veterinary and Animal Sciences 45/5 (2021), 881-889. https://doi.org/10.3906/vet-2010-26