Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks

TEKELI, MUHAMMET YASIN; Kanbur, Murat

doi:10.55730/1300-0128.4201

Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks

Muhammet Yasin TEKELİ, (Erciyes Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Kayseri, Türkiye)

Murat KANBUR (Erciyes Üniversitesi, Veteriner Fakültesi, Farmakoloji ve Toksikoloji Anabilim Dalı, Kayseri, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

3 0

Yıl: 2022 Cilt: 46 Sayı: 3 Sayfa Aralığı: 525 - 534 Metin Dili: İngilizce DOI: 10.55730/1300-0128.4201 İndeks Tarihi: 04-10-2022

Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks

Öz:

In this study, monensin (MON) was investigated for its apoptotic and genotoxic effects on chick liver tissue and cytotoxic and apoptotic effects on chick primary hepatocytes isolated from perfused liver tissue. Western blotting and real-time PCR (qPCR) were used to determine the apoptotic effect on primary hepatocytes and liver tissue, and the comet assay was used to determine the genotoxic effect on liver tissue. MON decreased cell viability in primary hepatocytes with increasing concentrations. When administered at concentrations of 1 and 10 µM, MON increased the levels of apoptotic p53, caspase-3, and caspase-9, and downregulated the expression of the antiapoptotic survivin, Bcl-xl, and Bcl-2 genes (p < 0.05). Furthermore, 100 and 125 ppm of MON increased caspase-3, caspase-9, and p53 protein levels, and downregulated Bcl-2 expression (p < 0.05). Bcl-xl gene expression was downregulated only in the group that received 125 ppm of MON (p < 0.05). Changes observed in the expression of the survivin gene were insignificant in both groups (p > 0.05). Moreover, 100 and 125 ppm of MON caused comet formation, which is a marker of genotoxicity, in the liver (p < 0.05). Our results indicate that MON induced apoptosis in both primary hepatocytes and liver tissue, and also caused genotoxicity in the liver tissue.

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APA	TEKELI M, Kanbur M (2022). Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. , 525 - 534. 10.55730/1300-0128.4201
Chicago	TEKELI MUHAMMET YASIN,Kanbur Murat Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. (2022): 525 - 534. 10.55730/1300-0128.4201
MLA	TEKELI MUHAMMET YASIN,Kanbur Murat Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. , 2022, ss.525 - 534. 10.55730/1300-0128.4201
AMA	TEKELI M,Kanbur M Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. . 2022; 525 - 534. 10.55730/1300-0128.4201
Vancouver	TEKELI M,Kanbur M Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. . 2022; 525 - 534. 10.55730/1300-0128.4201
IEEE	TEKELI M,Kanbur M "Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks." , ss.525 - 534, 2022. 10.55730/1300-0128.4201
ISNAD	TEKELI, MUHAMMET YASIN - Kanbur, Murat. "Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks". (2022), 525-534. https://doi.org/10.55730/1300-0128.4201

APA	TEKELI M, Kanbur M (2022). Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. Turkish Journal of Veterinary and Animal Sciences, 46(3), 525 - 534. 10.55730/1300-0128.4201
Chicago	TEKELI MUHAMMET YASIN,Kanbur Murat Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. Turkish Journal of Veterinary and Animal Sciences 46, no.3 (2022): 525 - 534. 10.55730/1300-0128.4201
MLA	TEKELI MUHAMMET YASIN,Kanbur Murat Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. Turkish Journal of Veterinary and Animal Sciences, vol.46, no.3, 2022, ss.525 - 534. 10.55730/1300-0128.4201
AMA	TEKELI M,Kanbur M Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. Turkish Journal of Veterinary and Animal Sciences. 2022; 46(3): 525 - 534. 10.55730/1300-0128.4201
Vancouver	TEKELI M,Kanbur M Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks. Turkish Journal of Veterinary and Animal Sciences. 2022; 46(3): 525 - 534. 10.55730/1300-0128.4201
IEEE	TEKELI M,Kanbur M "Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks." Turkish Journal of Veterinary and Animal Sciences, 46, ss.525 - 534, 2022. 10.55730/1300-0128.4201
ISNAD	TEKELI, MUHAMMET YASIN - Kanbur, Murat. "Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks". Turkish Journal of Veterinary and Animal Sciences 46/3 (2022), 525-534. https://doi.org/10.55730/1300-0128.4201