TY  - JOUR
TI  - Monensin induces apoptosis in the liver tissue and primary hepatocytes of chicks
AB  - In this study, monensin (MON) was investigated for its apoptotic and genotoxic effects on chick liver tissue and cytotoxic and
apoptotic effects on chick primary hepatocytes isolated from perfused liver tissue. Western blotting and real-time PCR (qPCR) were
used to determine the apoptotic effect on primary hepatocytes and liver tissue, and the comet assay was used to determine the genotoxic
effect on liver tissue. MON decreased cell viability in primary hepatocytes with increasing concentrations. When administered at
concentrations of 1 and 10 µM, MON increased the levels of apoptotic p53, caspase-3, and caspase-9, and downregulated the expression
of the antiapoptotic survivin, Bcl-xl, and Bcl-2 genes (p < 0.05). Furthermore, 100 and 125 ppm of MON increased caspase-3, caspase-9,
and p53 protein levels, and downregulated Bcl-2 expression (p < 0.05). Bcl-xl gene expression was downregulated only in the group that
received 125 ppm of MON (p < 0.05). Changes observed in the expression of the survivin gene were insignificant in both groups (p >
0.05). Moreover, 100 and 125 ppm of MON caused comet formation, which is a marker of genotoxicity, in the liver (p < 0.05). Our results
indicate that MON induced apoptosis in both primary hepatocytes and liver tissue, and also caused genotoxicity in the liver tissue.
AU  - TEKELI, MUHAMMET YASIN
AU  - Kanbur, Murat
DO  - 10.55730/1300-0128.4201
PY  - 2022
JO  - Turkish Journal of Veterinary and Animal Sciences
VL  - 46
IS  - 3
SN  - 1300-0128
SP  - 525
EP  - 534
DB  - TRDizin
UR  - http://search/yayin/detay/528875
ER  -