The aim of this study is the development of a coagglutination test for rapid diagnosis of infectious pancreatic necrosis (IPN) virus which causes an acute infectious viral disease with high mortality in young salmonid fish. Serotypes Sp, Ab, WB of the IPNV were cultivated in BF-2 cells and purified by linear sucrose density gradient centrifugation. Hyperimmune sera against purified IPNV serotypes were collected from immunized guinea pigs. Coagglutination test conjugates were prepared by using sensitized S. aureus and hyperimmune sera. Cell culture derived salmonid pathogens such as infectious hematopoietic necrosis virus (IHNV), viral haemorrhagic septicaemia virus (VHSV) and epizootic hematopoietic necrosis virus (EHNV) were used to determine the specificity of the test as control. Upward comparison of the preferability for the rapid diagnosis was also carried out with ELISA and RT-PCR. The validation studies of newly developed coagglutination test were carried out in accordance with the Manual of Diagnostic Tests for Aquatic Animals. Consequently, determined detection limit of the test was approximately 105.25 TCID50/mL for the Sp and Ab serotypes and 107.75 TCID50/mL for the WB in laboratory conditions. The results indicated that newly developed coagglutination test was compatible with ELISA and RT-PCR in positive cell culture supernatants. It was concluded that this test would be an economic, rapid and reliable method for the diagnosis of IPN virus in cell culture.

The purpose of this study is to examine the standards and guidelines recommended by institutions and organizations interested in psychological testing and measurement worldwide and to introduce the relevant literature to those concerned. Within the scope of this purpose, the content of updated test standards recommended by the American Educational Research Association, the American Psychological Association and the National Council on Educational Measurement, which are the pioneers of institutions that develop standards for psychological testing, were examined. Besides, test adaptation guidelines developed by the International Testing Commission were included, which provide comprehensive guidelines on test adaptation and usage of intercultural testing. The Test Analysis Model proposed by the European Federation of Psychologists’ Associations was also presented generally, and the standards specifically regarding psychological tests and measurement processes used in employment processes established by the International Standards Organization were examined. General evaluations regarding the listed guidelines and standards were made, and the contents of the guidelines and standards were introduced to the all researchers interested in psychological measurement.