The effects of pulsed magnetic field on the key elements responsible for synthesis and
destruction of elastin-collagen in diabetic lung tissue

TOKUŞ, Murat; cicek, figen; Ocal, Isil; GÜNAY, ISMAIL; TOKGÜN, ONUR; Coşkun, Çağıl

doi:10.5455/medscience.2021.10.332

The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue

Çağıl COŞKUN, (Çukurova Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Adana, Türkiye)

Onur TOKGUN, (Pamukkale Üniversitesi, Tıp Fakültesi, Tıbbi Genetik Anabilim Dalı, Denizli, Türkiye)

Murat TOKUŞ, (Çukurova Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Adana, Türkiye)

Figen ÇİÇEK, (Çukurova Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Adana, Türkiye)

Işıl ÖCAL, (Çukurova Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Adana, Türkiye)

İsmail GÜNAY (Çukurova Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Adana, Türkiye)

Medicine Science

2 0

Yıl: 2022 Cilt: 11 Sayı: 2 Sayfa Aralığı: 586 - 592 Metin Dili: İngilizce DOI: 10.5455/medscience.2021.10.332 İndeks Tarihi: 04-07-2022

The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue

Öz:

Changes in the expression levels of genes responsible for synthesis-destruction of elastin-collagen and the occurrence of lung diseases are in correlation. Although, diabetes-related complications are important health problems, the mechanism by which diabetes exerts this effect is unclear. On the other hand, although the effect of pulsed magnetic field (PMF) in lung diseases has been shown, its mechanism of action is unknown. The study aimed to determine the effects of PMF on the key regulator elements of the synthesis-destruction of elastin-collagen at the transcriptional level. Rats were divided into groups as control, control + PMF 10Hz, diabetes, diabetes + PMF 10Hz. PMF groups were exposed to 10 Hz PMF for four weeks. In diabetic conditions, ELN, ELANE, and COL1A1 genes were dysregulated at the transcriptional level as their levels were 14.23±2.56; 7.62±1.37 and 0.24±0.04, respectively. Dysregulated ELN gene expressions were decreased to 6.17±1.97 by PMF application. There were no meaningful changes in gene expressions in control + PMF 10 Hz groups. The present study shows that ELN, ELANE, and COL1A1 may play a key role at the transcriptional level in the mechanism of diabetic-lung diseases. In addition, it may be said that “PMF shows its effect by re-regulating the expression level of ELN gene in diabetic lung tissues”. In future studies, ELN gene-targeted PMF application methods may be developed. Moreover, PMF application might not affect the genes that are responsible for elastin-collagen synthesis-destruction in healthy lungs when the PMF is applied on different tissues for the treatment of various diseases.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Suki B, Stamenovic D, Hubmayr R. Lung parenchymal mechanics. Compr Physiol. 2011;1:1317-51.
2. Dunphy SE, Bratt JA, Akram KM, et al. Hydrogels for lung tissue engineering: Biomechanical properties of thin collagen-elastin constructs. J Mech Behav Biomed Mater. 2014;38:251-9.
3. Pelosi P, Rocco PR, Negrini D, et al. The extracellular matrix of the lung and its role in edema formation. An Acad Bras Cienc. 2007;79:285-97.
4. Laurent GJ. Lung collagen: more than scaffolding. Thorax. 1986; 41:418-28.
5. Maclay JD, McAllister DA, Rabinovich R, et al. Systemic elastin degradation in chronic obstructive pulmonary disease. Thorax. 2012;67:606-12.
6. Zhou Y, Horowitz JC, Naba A, et al. Extracellular matrix in lung development, homeostasis and disease. Matrix Biol. 2018;73:77-104.
7. Chen Y, Jiang P. Identification of key genes associated with idiopathic pulmonary fibrosis using bioinformatics analysis. 2016.
8. Imai K, Dalal SS, Chen ES, et al. Human collagenase (matrix metalloproteinase-1) expression in the lungs of patients with emphysema. Am J Respir Crit Care Med. 2001;163:786-91.
9. Nkyimbeng T, Ruppert C, Shiomi T, et al. Pivotal role of matrix metalloproteinase 13 in extracellular matrix turnover in idiopathic pulmonary fibrosis. PLoS One. 2013;8:73279.
10. Blaauboer ME, Boeijen FR, Emson CL, et al. Extracellular matrix proteins: a positive feedback loop in lung fibrosis? Matrix Biol. 2014;34:17-8.
11. Sahu I, Haque A, Weidensee B, et al. Recent Developments in mRNABased Protein Supplementation Therapy to Target Lung Diseases. Mol Ther. 2019; 27:803-23.
12. Tao Z, Shi A, Zhao J. Epidemiological Perspectives of Diabetes. Cell Biochem Biophys. 2015;73:181-5.
13. American Diabetes A. Economic Costs of Diabetes in the U.S. in 2017. Diabetes Care. 2018;41:917-28.
14. Deshpande AD, Harris-Hayes M, Schootman M. Epidemiology of diabetes and diabetes-related complications. Phys Ther. 2008; 88:1254-64.
15. Ehrlich SF, Quesenberry CP, Jr., Van Den Eeden SK, et al. Patients diagnosed with diabetes are at increased risk for asthma, chronic obstructive pulmonary disease, pulmonary fibrosis, and pneumonia but not lung cancer. Diabetes Care. 2010;33:55-60.
16. Hsia CC, Raskin P. Lung function changes related to diabetes mellitus. Diabetes Technol Ther. 2007; 9 Suppl 1:73-82.
17. Litonjua AA, Lazarus R, Sparrow D, et al. Lung function in type 2 diabetes: the Normative Aging Study. Respir Med. 2005;99:1583-90.
18. Khateeb J, Fuchs E, Khamaisi M. Diabetes and Lung Disease: A Neglected Relationship. Rev Diabet Stud. 2019;15:1-15.
19. Shupak NM, Prato FS, Thomas AW. Therapeutic uses of pulsed magneticfield exposure: A review. URSI Radio Science Bulletin. 2003:9-32.
20. Mert T. Pulsed magnetic field treatment as antineuropathic pain therapy. Rev Neurosci. 2017;28:751-8.
21. Sadlonova J, Korpas J, Vrabec M, et al. The effect of the pulsatile electromagnetic field in patients suffering from chronic obstructive pulmonary disease and bronchial asthma. Bratisl Lek Listy. 2002;103:260-5.
22. Sadlonova J, Korpas J, Salat D, et al. The effect of the pulsatile electromagnetic field in children suffering from bronchial asthma. Acta Physiol Hung. 2003; 90:327-34.
23. Coskun C, Ocal I, Gunay I. A Low-Frequency Pulsed Magnetic Field Reduces Neuropathic Pain by Regulating NaV1.8 and NaV1.9 Sodium Channels at the Transcriptional Level in Diabetic Rats. Bioelectromagnetics. 2021;42:357-70.
24. Mert T, Ocal I, Guzel AI, et al. Clodronate changes neurobiological effects of pulsed magnetic field on diabetic rats with peripheral neuropathy. Electromagn Biol Med. 2013;32:342-54.
25. Craner MJ, Klein JP, Renganathan M, et al. Changes of sodium channel expression in experimental painful diabetic neuropathy. Ann Neurol. 2002;52:786-92.
27. Raymaekers M, Smets R, Maes B, et al. Checklist for optimization and validation of real-time PCR assays. J Clin Lab Anal. 2009;23:145-51.
28. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2001;25:402-8.
29. Deslee G, Woods JC, Moore CM, et al. Elastin expression in very severe human COPD. Eur Respiratory J. 2009;34:324-31.
30. Hoff CR, Perkins DR, Davidson JM. Elastin gene expression is upregulated during pulmonary fibrosis. Connect Tissue Res. 1999;40:145-53.
31. Lehti TM, Silvennoinen M, Kivelä R, et al. Effects of streptozotocininduced diabetes and physical training on gene expression of extracellular matrix proteins in mouse skeletal muscle. Am J Physiol Endocrinol Metab. 2006;290:900-7.
32. Lu L, Zhang Q, Pu LJ, et al. Dysregulation of matrix metalloproteinases and their tissue inhibitors is related to abnormality of left ventricular geometry and function in streptozotocin-induced diabetic minipigs. Int J Exp Pathol. 2008;89:125-37.
33. Xu X, Su S, Wang X, et al. Obesity is associated with more activated neutrophils in African American male youth. Int J Obes (Lond). 2015;39:26-32.
34. Aggarwal N, Koepke J, Matamala N, et al. Alpha-1 Antitrypsin Regulates Transcriptional Levels of Serine Proteases in Blood Mononuclear Cells. Am J Respiratory Critical Care Med. 2016;193:1065-7.
35. Van Lunteren E, Moyer M. Gene expression profiling in the type 1 diabetes rat diaphragm. PLoS One. 2009;4:7832.
36. Van Lunteren E, Moyer M, Spiegler S. Alterations in lung gene expression in streptozotocin-induced diabetic rats. BMC Endocrine Disorders. 2014;14:5.
37. Cui N, Hu M, Khalil RA. Biochemical and Biological Attributes of Matrix Metalloproteinases. Prog Mol Biol Transl Sci. 2017;147:1-73.
38. Surse VM, Gupta J, Tikoo K. Esculetin induced changes in Mmp13 and Bmp6 gene expression and histone H3 modifications attenuate development of glomerulosclerosis in diabetic rats. J Mol Endocrinol. 2011;46:245-54.
39. Rosas IO, Richards TJ, Konishi K, et al. MMP1 and MMP7 as potential peripheral blood biomarkers in idiopathic pulmonary fibrosis. PLoS Med. 2008;5:93.
40. Konishi K, Gibson KF, Lindell KO, et al. Gene expression profiles of acute exacerbations of idiopathic pulmonary fibrosis. Am J Respir Crit Care Med. 2009;180:167-75.
41. Woode D, Shiomi T, D'Armiento J. Collagenolytic matrix metalloproteinases in chronic obstructive lung disease and cancer. Cancers (Basel). 2015;7:329-41.

APA	Coşkun Ç, TOKGÜN O, TOKUŞ M, cicek f, Ocal I, GÜNAY I (2022). The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. , 586 - 592. 10.5455/medscience.2021.10.332
Chicago	Coşkun Çağıl,TOKGÜN ONUR,TOKUŞ Murat,cicek figen,Ocal Isil,GÜNAY ISMAIL The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. (2022): 586 - 592. 10.5455/medscience.2021.10.332
MLA	Coşkun Çağıl,TOKGÜN ONUR,TOKUŞ Murat,cicek figen,Ocal Isil,GÜNAY ISMAIL The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. , 2022, ss.586 - 592. 10.5455/medscience.2021.10.332
AMA	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. . 2022; 586 - 592. 10.5455/medscience.2021.10.332
Vancouver	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. . 2022; 586 - 592. 10.5455/medscience.2021.10.332
IEEE	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I "The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue." , ss.586 - 592, 2022. 10.5455/medscience.2021.10.332
ISNAD	Coşkun, Çağıl vd. "The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue". (2022), 586-592. https://doi.org/10.5455/medscience.2021.10.332

APA	Coşkun Ç, TOKGÜN O, TOKUŞ M, cicek f, Ocal I, GÜNAY I (2022). The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. Medicine Science, 11(2), 586 - 592. 10.5455/medscience.2021.10.332
Chicago	Coşkun Çağıl,TOKGÜN ONUR,TOKUŞ Murat,cicek figen,Ocal Isil,GÜNAY ISMAIL The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. Medicine Science 11, no.2 (2022): 586 - 592. 10.5455/medscience.2021.10.332
MLA	Coşkun Çağıl,TOKGÜN ONUR,TOKUŞ Murat,cicek figen,Ocal Isil,GÜNAY ISMAIL The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. Medicine Science, vol.11, no.2, 2022, ss.586 - 592. 10.5455/medscience.2021.10.332
AMA	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. Medicine Science. 2022; 11(2): 586 - 592. 10.5455/medscience.2021.10.332
Vancouver	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue. Medicine Science. 2022; 11(2): 586 - 592. 10.5455/medscience.2021.10.332
IEEE	Coşkun Ç,TOKGÜN O,TOKUŞ M,cicek f,Ocal I,GÜNAY I "The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue." Medicine Science, 11, ss.586 - 592, 2022. 10.5455/medscience.2021.10.332
ISNAD	Coşkun, Çağıl vd. "The effects of pulsed magnetic field on the key elements responsible for synthesis and destruction of elastin-collagen in diabetic lung tissue". Medicine Science 11/2 (2022), 586-592. https://doi.org/10.5455/medscience.2021.10.332