Autologous Platelet Rich Plasma Have Positive Eff ect on Ram
Spermatozoa During Cryopreservation in Non-Breeding Season

Yilmaz, Mehmet Melih; Akkaşoğlu, Mustafa; Koca, Davut; SAĞIRKAYA, Hakan; Kılıç, Mehmet ali; Alçay, Selim; AKTAR, Ahmet

doi:10.9775/kvfd.2021.26763

Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season

Selim ALCAY, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Ahmet AKTAR, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Davut KOCA, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı, Bursa, Türkiye)

Mehmet Ali KILIC, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Kadın Hastalıkları ve Doğum Anabilim Dalı, Bursa, Türkiye)

Mustafa AKKASOGLU, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Dölleme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Melih YILMAZ, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Dölleme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Hakan SAĞIRKAYA (Bursa Uludağ Üniversitesi Veteriner Fakültesi Dölleme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

19 4

Yıl: 2022 Cilt: 28 Sayı: 2 Sayfa Aralığı: 229 - 234 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.26763 İndeks Tarihi: 04-07-2022

Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season

Öz:

Th e aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen aft er thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/ mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed aft er thawing. Th e results showed that 25x106/mL PRP group had positive eff ect on motility (61.67±3.81), membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P<0.05). It was determined that 25x106/mL PRP and 50x106/mL PRP groups were more eff ective than other groups in terms of mitochondrial membrane potential (69.50±1.93), acrosome integrity (78.04±2.65) and DNA integrity (5.33±0.92). Th e results of the study showed that autologous PRP has a protective eff ect in the cryopreservation of ram spermatozoa.

Anahtar Kelime:

Otolog Trombositten Zengin Plazma Üreme Mevsimi Dışında Koç Spermatozoalarının Kriyoprezervasyonu Üzerinde Olumlu Etkiye Sahiptir

Öz:

Bu çalışmanın amacı, otolog trombositten zengin plazma (PRP) ilave edilmiş yumurta sarılı sulandırıcılar eritme sonrası koç sperması üzerine etkilerini değerlendirmektir. Dokuz adet kıvırcık koçtan elde edilen sperma numuneleri dört eşit hacme bölündü ve farklı konsantrasyonlarda PRP içeren sulandırıcılar [PRP yok (Kontrol), 12.5x106/mL PRP, 25x106/mL PRP ve 50x106/mL PRP) ile sulandırıldı. Çözdürme sonrası motilite, plazma membranı fonsiyonel bütünlüğü, akrozomal bütünlük, mitokondriyal fonksiyon, DNA bütünlüğü ve malondialdehit konsantrasyonu (MDA) ölçüldü ve analiz edildi. Sonuç olarak, 25x106/mL PRP içeren sulandırcının çözdürme sonrası motilite (61.67±3.81), plazma membran fonksiyonel bütünlüğü (71.00±2.96), ve MDA seviyeleri (5.13±0.64) üzerine olumlu etkisi olduğunu gösterdi (P<0.05). 25x106/mL PRP ve 50x106/mL PRP gruplarının mitokondriyal fonksiyon (69.50±1.93), akrozomal bütünlüğü (78.04±2.65) ve DNA bütünlüğü (5.33±0.92) açısından diğer gruplara göre daha etkili olduğu belirlendi. Çalışmanın sonuçları, otolog PRP’nin koç spermasının dondurularak saklanması üzerine koruyucu bir etkiye sahip olduğu görüldü.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Alçay S, AKTAR A, Koca D, Kılıç M, Akkaşoğlu M, Yilmaz M, SAĞIRKAYA H (2022). Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. , 229 - 234. 10.9775/kvfd.2021.26763
Chicago	Alçay Selim,AKTAR Ahmet,Koca Davut,Kılıç Mehmet ali,Akkaşoğlu Mustafa,Yilmaz Mehmet Melih,SAĞIRKAYA Hakan Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. (2022): 229 - 234. 10.9775/kvfd.2021.26763
MLA	Alçay Selim,AKTAR Ahmet,Koca Davut,Kılıç Mehmet ali,Akkaşoğlu Mustafa,Yilmaz Mehmet Melih,SAĞIRKAYA Hakan Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. , 2022, ss.229 - 234. 10.9775/kvfd.2021.26763
AMA	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. . 2022; 229 - 234. 10.9775/kvfd.2021.26763
Vancouver	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. . 2022; 229 - 234. 10.9775/kvfd.2021.26763
IEEE	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H "Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season." , ss.229 - 234, 2022. 10.9775/kvfd.2021.26763
ISNAD	Alçay, Selim vd. "Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season". (2022), 229-234. https://doi.org/10.9775/kvfd.2021.26763

APA	Alçay S, AKTAR A, Koca D, Kılıç M, Akkaşoğlu M, Yilmaz M, SAĞIRKAYA H (2022). Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(2), 229 - 234. 10.9775/kvfd.2021.26763
Chicago	Alçay Selim,AKTAR Ahmet,Koca Davut,Kılıç Mehmet ali,Akkaşoğlu Mustafa,Yilmaz Mehmet Melih,SAĞIRKAYA Hakan Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.2 (2022): 229 - 234. 10.9775/kvfd.2021.26763
MLA	Alçay Selim,AKTAR Ahmet,Koca Davut,Kılıç Mehmet ali,Akkaşoğlu Mustafa,Yilmaz Mehmet Melih,SAĞIRKAYA Hakan Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.2, 2022, ss.229 - 234. 10.9775/kvfd.2021.26763
AMA	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(2): 229 - 234. 10.9775/kvfd.2021.26763
Vancouver	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(2): 229 - 234. 10.9775/kvfd.2021.26763
IEEE	Alçay S,AKTAR A,Koca D,Kılıç M,Akkaşoğlu M,Yilmaz M,SAĞIRKAYA H "Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.229 - 234, 2022. 10.9775/kvfd.2021.26763
ISNAD	Alçay, Selim vd. "Autologous Platelet Rich Plasma Have Positive Eff ect on Ram Spermatozoa During Cryopreservation in Non-Breeding Season". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/2 (2022), 229-234. https://doi.org/10.9775/kvfd.2021.26763