TY  - JOUR
TI  - Autologous Platelet Rich Plasma Have Positive Eff ect on Ram
Spermatozoa During Cryopreservation in Non-Breeding Season
AB  - Th e aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender
on ram semen aft er thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots
and mixed with diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/
mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and
malondialdehyde concentrations (MDA) were measured and analyzed aft er thawing. Th e results showed that 25x106/mL PRP group had
positive eff ect on motility (61.67±3.81), membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P<0.05).
It was determined that 25x106/mL PRP and 50x106/mL PRP groups were more eff ective than other groups in terms of mitochondrial
membrane potential (69.50±1.93), acrosome integrity (78.04±2.65) and DNA integrity (5.33±0.92). Th e results of the study showed that
autologous PRP has a protective eff ect in the cryopreservation of ram spermatozoa.
AU  - Yilmaz, Mehmet Melih
AU  - Akkaşoğlu, Mustafa
AU  - Koca, Davut
AU  - SAĞIRKAYA, Hakan
AU  - Kılıç, Mehmet ali
AU  - Alçay, Selim
AU  - AKTAR, Ahmet
DO  - 10.9775/kvfd.2021.26763
PY  - 2022
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 28
IS  - 2
SN  - 1300-6045
SP  - 229
EP  - 234
DB  - TRDizin
UR  - http://search/yayin/detay/529975
ER  -