Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus
mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

Hüraydın, Oğuzhan; AKTAR, Ahmet; GOKÇE, ELIF; NUR, ZEKARIYA; AKAL, ESER; Yilmaz, Mehmet Melih; ustuner, burcu; ÖNDER, Nail Tekin; duman, Muhammed; Alçay, Selim

doi:10.9775/kvfd.2021.26855

Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

Burcu USTUNER, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Selim ALCAY, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Elif GÖKÇE, (Namık Kemal Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Tekirdağ, Türkiye)

Melih YILMAZ, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Ahmet AKTAR, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı, Bursa, Türkiye)

Oğuzhan HURAYDIN, (Bursa Uludağ Üniversitesi Veteriner Fakültesi Üreme ve Suni Tohumlama Anabilim Dalı)

Muhammed DUMAN, (Uludağ Üniversitesi Veteriner Fakültesi Su Hayvanları Hastalıkları Anabilim Dalı, Bursa, Türkiye)

N. Tekin ÖNDER, (Kafkas Üniversitesi Veteriner Fakültesi, Üreme ve Suni Tohumlama Anabilim Dalı, Kars, Türkiye)

Eser AKAL, (Ondokuz Mayıs Üniversitesi Veteriner Fakültesi, Üreme ve Suni Tohumlama Anabilim Dalı, Samsun, Türkiye)

Zekariya NUR (Ondokuz Mayıs Üniversitesi Veteriner Fakültesi Dölleme ve Suni Tohumlama Anabilim Dalı, Samsun, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

8 1

Yıl: 2022 Cilt: 28 Sayı: 2 Sayfa Aralığı: 255 - 260 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.26855 İndeks Tarihi: 04-07-2022

Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm

Öz:

Th is study aimed to investigate the eff ect of the Rainbow trout seminal plasma (RTSP) supplemented (control, 10% or 15%) lyophilized extender on freezability of ram semen. Collected semen was pooled and split into two aliquots, and each of the ejaculates was diluted with fresh or lyophilized extenders with RTSP (0%, FC and LC; 10% F10 and L10or 15% F15 and L15) using two-step dilution method. Semen was frozen using the programmable freezing machine. Semen samples were examined for sperm motility, defective acrosomes, plasma membrane integrity and DNA fragmentation at native and post-thaw stage. Th e highest percentages of post-thaw motility and plasma integrity were observed in the F10, F15, and L15 (P<0.05). Th ere was no significant diff erence in the rate of postthaw defective acrosomes when the other extender groups were compared with the control. Th e highest percentage of post-thaw DNA fragmentation values were observed in the FC group, while the lowest DNA fragmentation was obtained in the F15 but only significant diff erent from FC and LC groups. In conclusion, the findings of this study show that the lyophilized extender with 15% RTSP added can be used successfully for freezing ram semen.

Anahtar Kelime:

Gökkuşağı Alabalığı (Oncorhynchus mykiss) Seminal Plazması İlave Edilmiş Liyofilize Sulandırıcı, Koç Spermasının Dondurulmasını İyileştirir

Öz:

Bu çalışma Gökkuşağı alabalığı seminal plazması (ASP) ilave edilmiş (kontrol, %10 ve %15) liyofilize sulandırıcının koç spermasının dondurulabilirliği üzerindeki etkisini belirlemeyi amaçlamıştır. Alınan sperma birleştirilerek, iki eşit hacime bölündü ve ejakülatların her biri, iki aşamalı sulandırma yöntemi kullanılarak ASP (%0, FC ve LC; %10 F10 ve L10 veya %15 F15 ve L15) ilave edilen taze ve liyofilize sulandırıcılarla sulandırıldı. Sperma programlanabilir dondurma makinesi kullanılarak donduruldu. Sperma numuneleri, taze ve eritme sonrası aşamada motilite, akrozom hasarı, plazma membran bütünlüğü ve DNA fragmentasyonu bakımından incelendi. Eritme sonrası en yüksek motilite ve plazma membrane bütünlüğü F10, F15 ve L15 gruplarında gözlendi (P<0.05). Eritme sonrası motilite değerlerine paralel olarak %10 ASP içeren liyofilize sulandırıcının plazma membran bütünlüğü, kontrol gruplarından daha yüksek bulundu. Sulandırıcı grupları kontrol grupları ile karşılaştırıldığında, eritme sonrası akrozom bozukluğu bakımından istatistiksel bir fark tespit edilmedi. Eritme sonrası DNA fragmentasyonu en yüksek FC grubunda gözlenirken, en düşük F15 grubunda elde edildi fakat yalnızca FC ve LC gruplarından istatistiksel olarak farklıydı (P<0.05). Sonuç olarak, bu araştırmanın bulguları %15 ASP ilave edilen liyofilize sulandırıcının koç spermasının dondurulmasında başarıyla kullanılabileceğini göstermektedir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ustuner b, Alçay S, GOKÇE E, Yilmaz M, AKTAR A, Hüraydın O, duman M, ÖNDER N, AKAL E, NUR Z (2022). Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. , 255 - 260. 10.9775/kvfd.2021.26855
Chicago	ustuner burcu,Alçay Selim,GOKÇE ELIF,Yilmaz Mehmet Melih,AKTAR Ahmet,Hüraydın Oğuzhan,duman Muhammed,ÖNDER Nail Tekin,AKAL ESER,NUR ZEKARIYA Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. (2022): 255 - 260. 10.9775/kvfd.2021.26855
MLA	ustuner burcu,Alçay Selim,GOKÇE ELIF,Yilmaz Mehmet Melih,AKTAR Ahmet,Hüraydın Oğuzhan,duman Muhammed,ÖNDER Nail Tekin,AKAL ESER,NUR ZEKARIYA Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. , 2022, ss.255 - 260. 10.9775/kvfd.2021.26855
AMA	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. . 2022; 255 - 260. 10.9775/kvfd.2021.26855
Vancouver	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. . 2022; 255 - 260. 10.9775/kvfd.2021.26855
IEEE	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z "Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm." , ss.255 - 260, 2022. 10.9775/kvfd.2021.26855
ISNAD	ustuner, burcu vd. "Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm". (2022), 255-260. https://doi.org/10.9775/kvfd.2021.26855

APA	ustuner b, Alçay S, GOKÇE E, Yilmaz M, AKTAR A, Hüraydın O, duman M, ÖNDER N, AKAL E, NUR Z (2022). Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28(2), 255 - 260. 10.9775/kvfd.2021.26855
Chicago	ustuner burcu,Alçay Selim,GOKÇE ELIF,Yilmaz Mehmet Melih,AKTAR Ahmet,Hüraydın Oğuzhan,duman Muhammed,ÖNDER Nail Tekin,AKAL ESER,NUR ZEKARIYA Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28, no.2 (2022): 255 - 260. 10.9775/kvfd.2021.26855
MLA	ustuner burcu,Alçay Selim,GOKÇE ELIF,Yilmaz Mehmet Melih,AKTAR Ahmet,Hüraydın Oğuzhan,duman Muhammed,ÖNDER Nail Tekin,AKAL ESER,NUR ZEKARIYA Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.28, no.2, 2022, ss.255 - 260. 10.9775/kvfd.2021.26855
AMA	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(2): 255 - 260. 10.9775/kvfd.2021.26855
Vancouver	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2022; 28(2): 255 - 260. 10.9775/kvfd.2021.26855
IEEE	ustuner b,Alçay S,GOKÇE E,Yilmaz M,AKTAR A,Hüraydın O,duman M,ÖNDER N,AKAL E,NUR Z "Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 28, ss.255 - 260, 2022. 10.9775/kvfd.2021.26855
ISNAD	ustuner, burcu vd. "Lyophilized Extender Supplemented with Rainbow Trout (Oncorhynchus mykiss) Seminal Plasma Improves Cryopreservation of Ram Sperm". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 28/2 (2022), 255-260. https://doi.org/10.9775/kvfd.2021.26855