In vitro evaluation of antimicrobial eects of Citrus limonum
essential oil on some endodontic pathogens

Alacam, Tayfun; ŞEKER, İLKE DOĞA; AKÇA, Gülçin

doi:10.5455/annalsmedres.2021.07.494

In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens

İlke Doğa ŞEKER, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Ankara, Türkiye)

Gülçin AKÇA, (Gazi Üniversitesi, Diş Hekimliği Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Tayfun ALAÇAM (Lokman Hekim Üniversitesi, Diş Hekimliği Fakültesi, Endodonti Anabilim Dalı, Ankara, Türkiye)

Annals of Medical Research

2 1

Yıl: 2022 Cilt: 29 Sayı: 5 Sayfa Aralığı: 427 - 433 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2021.07.494 İndeks Tarihi: 05-07-2022

In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens

Öz:

Aim: To investigate the antimicrobial eects of Citrus limonum and calcium hydroxide comparatively and to evaluate MIC, MBC, and MFC levels for some microorganismal strains of the root canal microbial ora. Materials and Methods: Enterococcus faecalis, Candida albicans, Staphylococcus aureus, Streptococcus mutans, and Escherichia coli standard strains were used. Microorganisms were cultivated and used for the evaluation of the antimicrobial eect of the dierent concentrations of the C.limonum and calcium hydroxide using disc diusion and agar well diusion methods. Then MIC, MBC, and MFC values were recorded. Results: Wider inhibition zones were obtained for C.limonum essential oil compared to calcium hydroxide. In the agar well diusion method, while the widest inhibition zone was obtained for C.albicans, the narrowest inhibition zone was observed for E.faecalis for C.limonum. For calcium hydroxide, the widest inhibition zone was in C.albicans cultures, however, the narrowest inhibition zone was observed for S.mutans. In the agar disc diffusion method, the widest inhibition zone was observed for C.albicans, and the narrowest inhibition zone was spotted in S.aureus for Citrus limonum. For calcium hydroxide, while the widest inhibition zone again reached for C.albicans, the narrowest inhibition zone was found for E.faecalis. The lowest MIC and MBC value for Citrus limonum was observed in S.mutans. For calcium hydroxide, the lowest MIC and MBC values were recorded for S.aureus, E.coli, and S.mutans. Conclusion: Data in experimental conditions showed that C.limonum essential oil may be an alternative candidate, and superior to calcium hydroxide in terms of antimicrobial activity for all chosen members of intracanal ora.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ŞEKER İ, AKÇA G, Alacam T (2022). In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. , 427 - 433. 10.5455/annalsmedres.2021.07.494
Chicago	ŞEKER İLKE DOĞA,AKÇA Gülçin,Alacam Tayfun In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. (2022): 427 - 433. 10.5455/annalsmedres.2021.07.494
MLA	ŞEKER İLKE DOĞA,AKÇA Gülçin,Alacam Tayfun In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. , 2022, ss.427 - 433. 10.5455/annalsmedres.2021.07.494
AMA	ŞEKER İ,AKÇA G,Alacam T In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. . 2022; 427 - 433. 10.5455/annalsmedres.2021.07.494
Vancouver	ŞEKER İ,AKÇA G,Alacam T In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. . 2022; 427 - 433. 10.5455/annalsmedres.2021.07.494
IEEE	ŞEKER İ,AKÇA G,Alacam T "In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens." , ss.427 - 433, 2022. 10.5455/annalsmedres.2021.07.494
ISNAD	ŞEKER, İLKE DOĞA vd. "In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens". (2022), 427-433. https://doi.org/10.5455/annalsmedres.2021.07.494

APA	ŞEKER İ, AKÇA G, Alacam T (2022). In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research, 29(5), 427 - 433. 10.5455/annalsmedres.2021.07.494
Chicago	ŞEKER İLKE DOĞA,AKÇA Gülçin,Alacam Tayfun In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research 29, no.5 (2022): 427 - 433. 10.5455/annalsmedres.2021.07.494
MLA	ŞEKER İLKE DOĞA,AKÇA Gülçin,Alacam Tayfun In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research, vol.29, no.5, 2022, ss.427 - 433. 10.5455/annalsmedres.2021.07.494
AMA	ŞEKER İ,AKÇA G,Alacam T In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research. 2022; 29(5): 427 - 433. 10.5455/annalsmedres.2021.07.494
Vancouver	ŞEKER İ,AKÇA G,Alacam T In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens. Annals of Medical Research. 2022; 29(5): 427 - 433. 10.5455/annalsmedres.2021.07.494
IEEE	ŞEKER İ,AKÇA G,Alacam T "In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens." Annals of Medical Research, 29, ss.427 - 433, 2022. 10.5455/annalsmedres.2021.07.494
ISNAD	ŞEKER, İLKE DOĞA vd. "In vitro evaluation of antimicrobial eects of Citrus limonum essential oil on some endodontic pathogens". Annals of Medical Research 29/5 (2022), 427-433. https://doi.org/10.5455/annalsmedres.2021.07.494