Investigation of biological activities of plant extract and green synthesis silver
nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits

Şahin, Birgütay; BÜLBÜL, Ali Savaş; Karadağ, Ahmet; ÇELİK, İBRAHİM SEYFETTİN; Korkmaz, Nesrin

doi:10.3906/kim-2108-48

Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits

Birgütay ŞAHİN, (Bartın Üniversitesi, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümü, Bartın, Türkiye)

Ali Savaş BÜLBÜL, (Bayburt Üniversitesi, Uygulamalı Bilimler Fakültesi, Acil Yardım ve Afet Yönetimi Bölümü, Bayburt, Türkiye)

İ. Seyfettin ÇELİK, (Kahramanmaraş Sütçü İmam Üniversitesi, Tıp Fakültesi, Tıbbi Patoloji Anabilim Dalı, Kahramanmaraş, Türkiye)

Nesrin KORKMAZ, (Yozgat Bozok Üniversitesi, Kenevir Araştırma Enstitüsü, Temel Bilimler ve Sağlık Anabilim Dalı, Yozgat, Türkiye)

Ahmet KARADAG (Yozgat Bozok Üniversitesi, Kenevir Araştırma Enstitüsü, Temel Bilimler ve Sağlık Anabilim Dalı, Yozgat, Türkiye)

Turkish Journal of Chemistry

9 0

Yıl: 2022 Cilt: 46 Sayı: 1 Sayfa Aralığı: 224 - 235 Metin Dili: İngilizce DOI: 10.3906/kim-2108-48 İndeks Tarihi: 05-07-2022

Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits

Öz:

In this study, biological activities of silver nanoparticles (AgNP) synthesized by green synthesis method using the fruit extract of Gilaburu (Viburnum opulus L.) plant were investigated. The characterization of the synthesized AgNPs was performed by ultraviolet visible region spectroscopy (UV-Vis), Fourier transform - infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and X-ray diffraction (XRD) techniques. The crystal size was found to be 52.32 nm when the XRD data were calculated with the DebyeScherrer’s equation. The antimicrobial activities of extract and AgNPs were investigated by microdilution and disk diffusion methods. Antibiofilm activities were examined by the crystal violet technique. The cytotoxic effects of the extract and AgNPs against MCF-7 (human breast cancer) and HUVEC (human umbilical vein endothelial cells) cell lines were evaluated by MTT assay. The IC50 values for the HUVEC line were found to be 0.97 mg/mL (AgNP) and 85.24 mg/mL (extract), while the IC50 values for the MCF-7 line were determined as 0.022 µg/mL (AgNP) and 0.021 µg/mL (extract). To our knowledge, this is the first report to comprehensively examine the biological activities of V. opulus L. extract and biosynthetic AgNPs.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Tuorkey MJ. Cancer therapy with phytochemicals: present and future perspectives. Biomedical and Environmental Science 2015; 28: 808–819.
2. Tan W, Lu J, Huang M, Li Y, Chen M et al. (2011). Anti-cancer natural products isolated from Chinese medicinal herbs. Chinese Medicine 2011; 6: 27.
3. Narayanan KB, Sakthivel N. (2010). Biological synthesis of metal nanoparticles by microbes. Advances in Colloid and Interface Science 2010; 156:1-13.
4. Duncan TV. Applications of Nanotechnology in food packaging and food safety: Barrier materials, antimicrobials and sensors. Journal of Colloid and Interface Science 2011; 363 (1): 1-24.
5. Kumar A, Chisti Y, Banerjee U. Synthesis of metallic nanoparticles using plant extracts. Biotechnology Advances 2013; 31: 346–356.
6. Vishnukumar P, Vivekanandhan S, Misra M, Mohanty AK. Recent advances and emerging opportunities in phytochemical synthesis of ZnO nanostructures. Materials Science in Semiconductor Processing 2018; 80: 143–161.
7. Board R. International Journal of Modern Engineering 2014; 4: 306–308.
8. Fernandez JG, Fernandez-Baldo AM, Berni E, Cami G, Duran N et al. “Production of silver nanoparticles using yeasts and evaluation of their antifungal activity against phyto pathogenic fungi”, Process Biochemistry 2016; 51: 1306–1313.
9. Barabadi H, Ovais M, Shinwari ZK, Saravanan M. Anticancer green bionanomaterials: present status and future prospects. Green Chemistry Letters and Reviews 2017; 10:285–314.
10. Parameshwaran R, Kalaiselvamb S., Jayavel R. Green synthesis of silver Nanoparticles using Beta vulgaris: role of process conditions on size distribution and surface structure. Materials Chemistry and Physics 2013; 140: 135–147.
11. Sheny DS, Mathew J, Philip D. Phytosynthesis of Au, Ag and Au–Ag bimetallic nanoparticles using aqueous extract and dried leaf of Anacardiumocci dentale. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2011; 79: 254–262.
12. Ramteke CH, Chakrabarti T, Sarangi BK, Pandy RA. (2013) Synthesis of silver nanoparticles from aqueous extract of leaves of Ocimum sanctum for enhanced antibacterial activity. Journal of Chemistry. 2013; 2013: 1-7. doi: 10.1155/2013/27892 5
13. Roopana SM, Rohita MG, Rahuman AA, Kamaraj C, Bharathi A, Surendraa TV. Low-cost and eco-friendly phyto-synthesis of silver nanoparticles using Cocos nucifera coir extract and its larvicidal activity. Industrial Crops and Products 2013; 43: 631–635.
14. Satyavani K, Gurudeeban S, Ramanathan T, Balasubramanian T. Toxicity study of silver nanoparticle synthesis from suaeda monoica on Hep-2 Cell Line. Avicenna Journal of Medical Biotechnology 2012; 4: 35–39.
15. Mason C, Vivekanandhan S, Misra M, Mohanty AK. Switchgrass (Panicum virgatum) extract mediated green synthesis of silver nanoparticles. World Journal of Nano Science and Engineering 2012; 2:47–52
16. Sadat-Shandiz SA, Shafiee-Ardestani M, Shahbazzadeh D, Assadi A, Ahangari Cohan R et al. Novel imatinib loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells. Artifical Cells Nanomedicine and Biotechnology 2017; 45: 1082–1091.
17. Jeyaraj M, Sathishkumar G, Sivanandhan G, Mubarakali D, Rajesh M et al. Biogenic silver nanoparticles for cancer treatment: an experimental report. Colloids and Surfaces B: Biointerfaces 2013; 106:86–92.
18. Atanasov AG, Waltenberger B, Wenzig EMP, Linder T, Wawrosch C et al. Discovery and resupply of pharmacologically active plant-derived natural products: a review. Biotechnology Advances 2015; 33:1582-1614.
19. Loh XJ, Lee TC, Dou Q, Deen GR. Utilising inorganic nanocarriers for gene delivery, Biomaterials Science 2016; 4: 70–86.
20. Dou QQ, Teng CP, Ye E, Loh XJ. Effective near-infrared photodynamic therapy assisted by upconversion nanoparticles conjugated with photosensitizers, International Journal of Nanomedicine 2015; 10: 419–432.
21. Şahin B, Demir E, Aygün A, Gündüz H, Şen F. Investigation of the effect of pomegranate extract and monodisperse silver nanoparticle combination on MCF-7 cell line, Journal of Biotechnology 2017; 260: 79–83.
22. Dennehy C. The Use of Herbs and Dietary Supplements in Gynecology: An Evidence-Based Review, Journal of Midwifery & Womens Health 2006; 51: 402–409.
23. Karaçelik AA, Küçük M, Iskefiyeli Z, Aydemir S, De Smet S et al. Antioxidant components of Viburnum opulus L. determined by on-line HPLC–UV–ABTS radical scavenging and LC–UV–ESI-MS methods, Food Chemistry 2015; 175: 106–114.
24. Moldovan B, Ghic O, David L, Chişbora C. The influence of storage on the total phenols content and antioxidant activity of the cranberrybush (Viburnum opulus L.) Fruits Extract, Revista De Chimie (Bucharest, Romania) 2012; 63: 463–464.
25. Moldovan B, David L, Chişbora C, Cimpoiu C. Degradation Kinetics of anthocyanins from European Cranberrybush (Viburnum opulus L.) fruit extracts. Effects of temperature, pH and storage solvent, Molecules 2012; 17: 11655–11666.
26. Altun ML, Çitoğlu GS, Yılmaz BS, Özbek H. Antinociceptive and anti-inflammatory activities of Viburnum opulus, Pharmaceutical Biology 2009; 47: 653–658.
27. Khalil MMH, Ismail EH, El-Magdoub F. Biosynthesis of Au nanoparticles using olive leaf extract: 1st Nano Updates, Arabian Journal of Chemistry 2012; 5: 431–437.
28. Gurunathan S, Choi YJ, Kim JH. Antibacterial efficacy of silver nanoparticles on endometritis caused by Prevotella melaninogenica and Arcanobacterum pyogenes in dairy cattle, International Journal of Molecular Sciences 2018; 19 (4): 1210.
29. Hudzicki J. Kirby-Bauer disk diffusion susceptibility test protocol. American Society for Microbiology 2009; 2016: 1-23.
30. Merritt JH, Kadouri DE, O’Toole GA. Growing and analyzing static biofilms. Current Protocols in Microbiology 2011; 22: 1-18.
31. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 1998; 65: 55-63.
32. Denizot F, Lang R. Rapid colorimetric assay for cell growth and survival modifications to the tetrazolium dye procedure giving improved sensitivity and reliability. Journal of Immunological Methods, 1986; 89: 271-277.
33. Holst-Hansen C, Brünner N. MTT cell proliferation assay. Cell Biology. A Laboratory Handbook. Edt: Celis, J.E.,1998: 16-18.
34. Banerjee P, Satapathy M, Mukhopahayay A, Das P. Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresources and Bioprocessing, 2014; 1 (1): 3.
35. Gutierrez RMP, Mendez JVM, Vazquez IA. Chapter 2- A novel approach to the oral delivery of bionanostructures for systemic disease. Nanostructures for Oral Medicine 2017: 27-59. doi: 10.1016/B978-0-323-47720-8.00002-X
36. Murthy SK. Nanoparticles in modern medicine: state of the art and future challenges. International Journal of Nanomedicine, 2007; 2 (2): 129-141.
37. Vilchis-Nestor AR, Sánchez-Mendieta V, Camacho-López MA, Gómez- Espinosa RM, Arenas-Alatorre JA. Solventless synthesis and optical properties of Au and Ag nanoparticles using Camellia sinensis extract. Materials Letters 2008; 62: 3103–3105.
38. Ahmed S, Ahmad M, Swami BL, Ikram S. Green synthesis of silver nanoparticles using Azadirachta indica aqueous leaf extract. Journal of Radiation Research and Applied Science, 2016; 9: 1–7.
39. Das R, Nath SS, Chakdar D, Cope G, Bhattacharjee R. Preparation of silver nanoparticles and their characterization. Journal of Nanotechnology Online 2009; 5: 1–6.
40. Jahan I. Green synthesis of metal nanoparticles, their chemical and biochemical characterizations. PhD Dissertation. Yıldız Technical University Graduate School of Natural and Applied Sciences. Department of Bioengineering. Program of Bioengineering. İstanbul, Turkey. 2019; 99.
41. Davarnia B, Shahidi SA, Karimi-Maleh H, Ghorbani-Hasansaraei A, Karimi F. Biosynthesis of Ag Nanoparticle by Peganum Harmala Extract; Antimicrobial Activity and Ability for Fabrication of Quercetin Food Electrochemical Sensor, International Journal of Electrochemical Science 2020; 15:2549–2560.
42. Korkmaz N, Ceylan Y, Karadağ A, Bülbül AS, Aftab MN et al. Biogenic silver nanoparticles synthesized from Rhododendron ponticum and their antibacterial, antibiofilm and cytotoxic activities. Journal of Pharmaceutical and Biomedical Analysis 2020; 179: 112993.
43. Korkmaz N. Antibacterial Activity and Biofilm Property of Silver Nanoparticles Synthesized by Using Saintpaulia Aqueous Leaf Extract. Journal of the Institute of Science and Technology 2019; 9: 2225–2235.
44. Korkmaz N. Bioreduction; The biological activity, characterization and synthesis of silver nanoparticles, Turkish Journal of Chemistry 2020; 44: 325–334.
45. Jyoti K, Baunthiyal M, Singh A. Characterization of silver nanoparticles synthesized using Urtica dioica Linn. leaves and their synergistic effects with antibiotics. Journal of Radiation Research and Applied Science 2016; 9: 217–227.
46. Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science 2009; 145: 83–96.
47. Andrews JM. Determination of minimum inhibitory concentrations. Journal of Antimicrobial Chemotherapy, 2002; 49: 1049.
48. Erdogan O, Abbak M, Demirbolat GM, Birtekocak F, Aksel M et al. Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells. PLoS One 2019; 14 (6): e0216-496.
49. Safaepour M, Shahverdi AR, Shahverdi HR, Khorramizadeh MR, Gohari AR. Green synthesis of small silver nanoparticles using geraniol and ıts cytotoxicity against fibrosarcoma-wehi 164. Avicenna Journal Medical Biotechnology 2009; 1 (2): 111-115.
50. Sriram MI, Kanth SBM, Kalishwaralal K, Gurunathan S. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. International Journal of Nanomedicine 2010; 5: 753-762.
51. Takahashi A, Masuda A, Sun M, Centonze VE, Herman B. Oxidative stress induced apoptosis is associated with alterations in mitochondrial caspase activity and Bcl-2-dependent alterations in mitochondrial pH (pHm). Brain Research Bulletin 2004; 62: 497-504.
52. Korkmaz N, Berkil Akar K, İmamoğlu R, Kısa D, Karadağ A. Synthesis of silver nanowires in a two-phase system for biological Applications. Applied Organometallic Chemistry 2021; e6213. doi: 10.1002/aoc.6213

APA	Şahin B, BÜLBÜL A, ÇELİK İ, Korkmaz N, Karadağ A (2022). Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. , 224 - 235. 10.3906/kim-2108-48
Chicago	Şahin Birgütay,BÜLBÜL Ali Savaş,ÇELİK İBRAHİM SEYFETTİN,Korkmaz Nesrin,Karadağ Ahmet Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. (2022): 224 - 235. 10.3906/kim-2108-48
MLA	Şahin Birgütay,BÜLBÜL Ali Savaş,ÇELİK İBRAHİM SEYFETTİN,Korkmaz Nesrin,Karadağ Ahmet Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. , 2022, ss.224 - 235. 10.3906/kim-2108-48
AMA	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. . 2022; 224 - 235. 10.3906/kim-2108-48
Vancouver	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. . 2022; 224 - 235. 10.3906/kim-2108-48
IEEE	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A "Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits." , ss.224 - 235, 2022. 10.3906/kim-2108-48
ISNAD	Şahin, Birgütay vd. "Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits". (2022), 224-235. https://doi.org/10.3906/kim-2108-48

APA	Şahin B, BÜLBÜL A, ÇELİK İ, Korkmaz N, Karadağ A (2022). Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. Turkish Journal of Chemistry, 46(1), 224 - 235. 10.3906/kim-2108-48
Chicago	Şahin Birgütay,BÜLBÜL Ali Savaş,ÇELİK İBRAHİM SEYFETTİN,Korkmaz Nesrin,Karadağ Ahmet Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. Turkish Journal of Chemistry 46, no.1 (2022): 224 - 235. 10.3906/kim-2108-48
MLA	Şahin Birgütay,BÜLBÜL Ali Savaş,ÇELİK İBRAHİM SEYFETTİN,Korkmaz Nesrin,Karadağ Ahmet Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. Turkish Journal of Chemistry, vol.46, no.1, 2022, ss.224 - 235. 10.3906/kim-2108-48
AMA	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. Turkish Journal of Chemistry. 2022; 46(1): 224 - 235. 10.3906/kim-2108-48
Vancouver	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits. Turkish Journal of Chemistry. 2022; 46(1): 224 - 235. 10.3906/kim-2108-48
IEEE	Şahin B,BÜLBÜL A,ÇELİK İ,Korkmaz N,Karadağ A "Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits." Turkish Journal of Chemistry, 46, ss.224 - 235, 2022. 10.3906/kim-2108-48
ISNAD	Şahin, Birgütay vd. "Investigation of biological activities of plant extract and green synthesis silver nanoparticles obtained from Gilaburu (Viburnum opulus L.) fruits". Turkish Journal of Chemistry 46/1 (2022), 224-235. https://doi.org/10.3906/kim-2108-48