TY  - JOUR
TI  - Isolation and Characterization of Lytic Bacteriophages from Wastewater
with Phage Therapy Potentials Against Gram-Negative Bacteria
AB  - Objective: The increase of multidrug resistance in bacteria has increased the efforts in search of alternative
methods. The aim of the present study was to isolate and characterize the lytic phages and assess their lytic
activity against a number of gram-negative bacteria.
Materials and Methods: The phages and their respective hosts were isolated from wastewater collected
from the municipal sewer system of Trabzon, Turkey. The lytic activities of phage were determined using
the agar spot test. The identification and antibiotic susceptibility of host bacteria were determined using
matrix-assisted laser desorption ionization time-of-flight mass spectrometry and Phoenix 100, respectively.
The phages were characterized morphologically using transmission electron microscopy. One of the phages,
Enteroc21, which has a broad-host-range, was further characterized by genome restriction endonuclease
analysis and burst size.
Results: Two phages infected strains of four different species, nine phages were able to infect 2-4 strains
belonging to one or two species, and three phages showed lytic activity against only the hosts from which
they were isolated. All phages belonged to the Siphoviridae, Myoviridae, and Podoviridae family based on transmission
electron microscopy morphology. The Enteroc21 had more than 100 kb genome size and a burst
size of 180 per infected cell. Most of the host strains were resistant to ampicillin, amoxicillin–clavulanic
acid, and in particular, Achromobacter xylosoxidans TRAX 13 was multidrug-resistant showing resistance to
cefepime, aztreonam, gentamicin, netilmicin, and ciprofloxacin.
Conclusion: This study showed that the isolated phages have the potential to be used in phage therapy
against various bacterial infections, including multidrug-resistant bacteria.
AU  - NAS, Seyran Sakine
AU  - Kilic, Ali Osman
AU  - Durukan, İnci
AU  - TÜFEKCİ, Enis Fuat
AU  - KHORSHIDTALAB, MONA
AU  - Abdurahman, Mujib Abdulkadir
DO  - 10.5152/eurasianjmed.2022.21010
PY  - 2022
JO  - Eurasian Journal of Medicine
VL  - 54
IS  - 2
SN  - 1308-8734
SP  - 157
EP  - 164
DB  - TRDizin
UR  - http://search/yayin/detay/530135
ER  -