TY  - JOUR
TI  - The effect of vitamin D deficiency and 1,25(OH)2D3 treatment on
oxidative stress and Nrf2-antioxidant signaling in ethanol-induced
hepatotoxicity
AB  - Aim: Vitamin D deficiency (VDD) is suggested to enhance hepatotoxicity in chronic liver diseases. However,
there is limited knowledge about the association between VDD and alcoholic liver damage. Therefore, the effect
of VDD on ethanol (EtOH)-induced hepatotoxicity was investigated in this study. Moreover, the role of the
Nrf2-antioxidant signaling pathway in the hepatoprotective potential of 1,25(OH)2D3 was also searched in
EtOH-treated rats.
Methods: Male Wistar rats were fed on VDD-diet for 12 weeks. EtOH (5-20%) was applied in drinking water in
increasing concentrations for the last 8 weeks. In addition, one group of rats were injected with 1,25(OH)2D3
(5μg/kg; twice a week; i.p.) during this period. Hepatic triglyceride and hydroxyproline levels, inflammation
markers, lipid peroxides, protein carbonyls, mRNA expressions of Nrf2, superoxide dismutase (SOD), and
glutathione peroxidase (GSH-Px), SOD and GSH-Px activities, glutathione levels and histopathology were
examined.
Results: EtOH application caused steatosis and fibrosis, elevated hepatic TG, lipid peroxide, protein carbonyls
and hydroxyproline levels and inflammation markers. VDD did not aggravate EtOH-induced liver damage,
steatosis and inflammation, but reactive oxygen species and lipid peroxide levels were slightly increased in
VDD+EtOH group. Gene expressions of Nrf2-SOD-GSH-Px, enzyme activities and glutathione levels were also
higher in VDD+EtOH group than EtOH group. Additionally, 1,25(OH)2D3 elevated mRNA expressions and
activities of SOD and GSH-Px in EtOH-treated rats.
Conclusion: Our results indicate that VDD diet did not cause an additive effect on EtOH-induced hepatotoxicity.
Moreover, it was detected that the activation of Nrf2-antioxidant signaling pathway may play a role in the
protective effect of 1,25(OH)2D3 against EtOH-induced hepatotoxicity.
AU  - Dogru-Abbasoglu, Semra
AU  - Doğan Ekici, Asiye Işın
AU  - BINGÜL, ILKNUR
AU  - Uysal, Mujdat
AU  - KUCUKGERGIN, CANAN
AU  - Aydın, Abdurrahman Fatih
DO  - 10.25000/acem.949073
PY  - 2021
JO  - Archives of Clinical and Experimental Medicine
VL  - 6
IS  - 3
SN  - 2564-6567
SP  - 127
EP  - 133
DB  - TRDizin
UR  - http://search/yayin/detay/530584
ER  -