Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms 
of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?

AKGOL GUR, Sultan Tuna; kocak, abdullah osman; leylek, habip emrah; Akbaş, İlker; cokbankir, ozge; AYGORMEZ, Serpil; Dogruyol, Sinem

doi:10.4274/eajem.galenos.2021.45467

Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?

Sinem DOĞRUYOL, (Manisa Merkez Efendi Devlet Hastanesi, Acil Tıp Anabilim Dalı, Manisa, Türkiye)

İlker AKBAŞ, (Bingöl Devlet Hastanesi, Acil Tıp Anabilim Dalı, Bingöl, Türkiye)

Abdullah Osman KOÇAK, (Atatürk Üniversitesi Tıp Fakültesi Acil Tıp Anabilim Dalı, Erzurum, Türkiye)

Serpil AYGÖRMEZ, (Kafkas Üniversitesi Veteriner Fakültesi Biyokimya Anabilim Dalı, Kars, Türkiye)

Habip Emrah LEYLEK, (Bandırma Devlet Hastanesi, Acil Tıp Anabilim Dalı, Balıkesir, Türkiye)

Sultan Tuna AKGÖL GÜR, (Atatürk Üniversitesi Tıp Fakültesi Acil Tıp Anabilim Dalı, Erzurum, Türkiye)

Özge ERTENER (İzmir Ekonomi Üniversitesi Tıp Fakültesi Patoloji Anabilim Dalı, İzmir, Türkiye)

Eurasian Journal of Emergency Medicine

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Yıl: 2022 Cilt: 21 Sayı: 2 Sayfa Aralığı: 132 - 137 Metin Dili: İngilizce DOI: 10.4274/eajem.galenos.2021.45467 İndeks Tarihi: 06-07-2022

Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?

Öz:

Aim: The aim of this study was to examine the basic mechanisms that play a role in the acute nephrotoxicity caused by diclofenac sodium. Materials and Methods: Only water was given to the control group; however, the diclofenac sodium group was group intoxicated by giving water-soluble, 240 mg/kg, oral single dose diclofenac sodium. After 24 hours, all animals were sacrificed and histopathological analyzes were performed. The levels of spesific biomarkers [vascular endothelial growth factor (VEGF), nuclear factor-kappa B (NF-κB), matrix metalloproteinase-9 (MMP-9), metalloproteinase tissue inhibitor-1 (TIMP-1) and carcinoembryonic antigen (CEA)] that may be related to the nephrotoxicity mechanism were evaluated. Results: As a result of biochemical analysis, we found that VEGF, TIMP-1, NF-κB and CEA levels were significantly higher and MMP-9 levels were significantly lower in diclofenac sodium group compared to control group. Nephrotoxicity related histopathological changes were observed in the sections of diclofenac sodium group. Conclusion: This study has shown that the biomarkers we evaluated in the diclofenac sodium-induced acute high-dose intoxication model we created can help us to identify the nephrotoxicity and to explain the nephrotoxicity mechanism with the three main steps (the hemodynamicrelated pathway, the inflammation-related pathway, and the oxidative stress-related pathway). With a simple version of this panel adapted to emergency departments, we may be able to diagnose diclofenac sodium-related nephrotoxicity.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Dogruyol S, Akbaş İ, kocak a, AYGORMEZ S, leylek h, AKGOL GUR S, cokbankir o (2022). Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. , 132 - 137. 10.4274/eajem.galenos.2021.45467
Chicago	Dogruyol Sinem,Akbaş İlker,kocak abdullah osman,AYGORMEZ Serpil,leylek habip emrah,AKGOL GUR Sultan Tuna,cokbankir ozge Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. (2022): 132 - 137. 10.4274/eajem.galenos.2021.45467
MLA	Dogruyol Sinem,Akbaş İlker,kocak abdullah osman,AYGORMEZ Serpil,leylek habip emrah,AKGOL GUR Sultan Tuna,cokbankir ozge Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. , 2022, ss.132 - 137. 10.4274/eajem.galenos.2021.45467
AMA	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. . 2022; 132 - 137. 10.4274/eajem.galenos.2021.45467
Vancouver	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. . 2022; 132 - 137. 10.4274/eajem.galenos.2021.45467
IEEE	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o "Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?." , ss.132 - 137, 2022. 10.4274/eajem.galenos.2021.45467
ISNAD	Dogruyol, Sinem vd. "Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?". (2022), 132-137. https://doi.org/10.4274/eajem.galenos.2021.45467

APA	Dogruyol S, Akbaş İ, kocak a, AYGORMEZ S, leylek h, AKGOL GUR S, cokbankir o (2022). Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. Eurasian Journal of Emergency Medicine, 21(2), 132 - 137. 10.4274/eajem.galenos.2021.45467
Chicago	Dogruyol Sinem,Akbaş İlker,kocak abdullah osman,AYGORMEZ Serpil,leylek habip emrah,AKGOL GUR Sultan Tuna,cokbankir ozge Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. Eurasian Journal of Emergency Medicine 21, no.2 (2022): 132 - 137. 10.4274/eajem.galenos.2021.45467
MLA	Dogruyol Sinem,Akbaş İlker,kocak abdullah osman,AYGORMEZ Serpil,leylek habip emrah,AKGOL GUR Sultan Tuna,cokbankir ozge Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. Eurasian Journal of Emergency Medicine, vol.21, no.2, 2022, ss.132 - 137. 10.4274/eajem.galenos.2021.45467
AMA	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. Eurasian Journal of Emergency Medicine. 2022; 21(2): 132 - 137. 10.4274/eajem.galenos.2021.45467
Vancouver	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?. Eurasian Journal of Emergency Medicine. 2022; 21(2): 132 - 137. 10.4274/eajem.galenos.2021.45467
IEEE	Dogruyol S,Akbaş İ,kocak a,AYGORMEZ S,leylek h,AKGOL GUR S,cokbankir o "Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?." Eurasian Journal of Emergency Medicine, 21, ss.132 - 137, 2022. 10.4274/eajem.galenos.2021.45467
ISNAD	Dogruyol, Sinem vd. "Can Spesific Biomarkers Be Used to Enlighten the Major Mechanisms of Acute High Dose Diclofenac Sodium-Related Nephrotoxicity?". Eurasian Journal of Emergency Medicine 21/2 (2022), 132-137. https://doi.org/10.4274/eajem.galenos.2021.45467