SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients

Yıl: 2020 Cilt: 44 Sayı: 3 Sayfa Aralığı: 192 - 202 Metin Dili: İngilizce DOI: 10.3906/biy-2004-113 İndeks Tarihi: 07-07-2022

SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients

Öz:
The novel coronavirus pneumonia, which was named later as coronavirus disease 2019 (COVID-19), is caused by the severe acute respiratory syndrome coronavirus 2, namely SARS-CoV-2. It is a positive-strand RNA virus that is the seventh coronavirus known to infect humans. The COVID-19 outbreak presents enormous challenges for global health behind the pandemic outbreak. The first diagnosed patient in Turkey has been reported by the Republic of Turkey Ministry of Health on March 11, 2020. In May, over 150,000 cases in Turkey, and 5.5 million cases around the world have been declared. Due to the urgent need for a vaccine and antiviral drug, isolation of the virus is crucial. Here, we report 1 of the first isolation and characterization studies of SARS-CoV-2 from nasopharyngeal and oropharyngeal specimens of diagnosed patients in Turkey. This study provides an isolation and replication methodology,and cell culture tropism of the virus that will be available to the research communities.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Andersen KG, Rambaut A, Lipkin WI, Holmes EC, Garry RF (2020). The proximal origin of SARS-CoV-2. Nature Medicine 26: 450- 52. doi: 10.1038/s41591-020-0820-9
  • Baer A, Kylene KH (2014). Viral concentration determination through plaque assays: using traditional and novel overlay systems. Journal of Visualized Experiments 93: 1-10. doi: 10.3791/52065
  • Cui J, Fang L, Zheng LS (2019). Origin and evolution of pathogenic coronaviruses. Nature Reviews Microbiology 17: 181-192. doi: 10.1038/s41579-018-0118-9
  • Guallart N (2015). An overview of virus qunatification techniques. Axiomathes 25: 61-77. doi: 10.1007/s10516-014-9260-9
  • Harcourt J, Tamin A, Lu X, Kamili S, Kumar S (2020). Isolation and characterization of SARS-CoV-2 from the first US COVID-19 patient. bioRxiv doi: https://doi.org/10.1101/2020.03.02.972935
  • He L, Ding Y, Zhang Q, Che X, He Y et al. (2006). Expression of elevated levels of pro-inflammatory cytokines in SARS-CoVinfected ACE2+ cells in SARS patients: relation to the acute lung injury and pathogenesis of SARS288–97. The Journal of Pathology 210 (3): 288-297. doi: 10.1002/path
  • Holshue ML, DeBoltC, Lindquist S, Lofy KH, Wiesman J et al. (2020). First case of 2019 novel coronavirus in the United States. The New England Journal of Medicine 382 (10): 929- 936. doi: 10.1056/NEJMoa2001191
  • Honda-Okubo Y, Barnard D, Ong CH, Peng BH, Kent Tseng CT et al. (2015). Severe acute respiratory syndrome-associated coronavirus vaccines formulated with delta inulin adjuvants provide enhanced protection while ameliorating lung eosinophilic immunopathology. Journal of Virology 89 (6): 2995-3007. doi: 10.1128/jvi.02980-14
  • Hui DS, Azhar EI, Madani TA, Ntoumi F, Kock R (2020). The continuing 2019-NCoV epidemic threat of novel cCoronaviruses to global health — the latest 2019 novel coronavirus outbreak in Wuhan, China. International Journal of Infectious Diseases 91: 264-266. doi: 10.1016/j. ijid.2020.01.009
  • Li CK, Wu H, Yan H, Ma S, Wang L et al. (2008). T cell responses to whole SARS coronavirus in humans.The Journal of Immunology 181 (8): 5490-5500. doi: 10.4049/jimmunol.181.8.5490
  • Li Q, Guan X, Wu P, Wang X, Zhou L et al. (2020). Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia. The New England Journal of Medicine 382 (13): 1199-1207. doi: 10.1056/NEJMoa2001316
  • Libraty DH, O’Neil KM, Baker LM, Acosta LP, Olveda RM (2007). Human CD4+ memory T-lymphocyte responses to SARS coronavirus infection.Virology 368 (2): 317-321. doi: 10.1016/j. virol.2007.07.015
  • Liu W, Li H (2020). COVID-19: Attacks the 1-beta chain of hemoglobin and captures the porphyrin to inhibit human meme metabolism. ChemRxiv. doi: 10.26434/chemrxiv
  • Masters PS, Perlman S (2013). Coronaviridae. In: Knipe DM, Howley PM, Cohen JI, Griffen DE, Lamb RA et al. (editors). Fields Virology, Vol. 1. 6th ed. Philadelphia, PA, USA: Lippincott Williams & Wilkins, pp. 825-858.
  • Matsumoto M, Mukai T, Furukawa S, Ohori H (2005). Inhibitory effects of epigallocatechin gallate on the propagation of bovine coronavirus in Madin-Darby bovine kidney cells. Animal Science Journal 76 (5): 507-512. doi: 10.1111/j.1740- 0929.2005.00297.x
  • Paraskevis D, Kostaki EG, Magiorkinis G, Panayiotakopoulos G, Sourvinos G et al. (2020). Full-genome evolutionary analysis of the novel corona virus (2019-NCoV) rejects the hypothesis of emergence as a result of a recent recombination event. Infection, Genetics and Evolution 79: 1004212. doi: 10.1016/j. meegid.2020.104212
  • Stohl W, Mayer L (1987). Inhibition of T cell-dependent human B cell proliferation and B cell differentiation by polyspecific monomeric IgG. Clinical and Experimental Immunology 70 (3): 649-57.
  • Weiss SR, Leibowitz JL (2011). Chapter 4 - Coronavirus pathogenesis. Advances in Virus Research 81: 85-164. doi: 10.1016/B978-0- 12-385885-6.00009-2
  • Wong G, Liu W, Liu Y, Zhou B, Bi Y et al. (2015). MERS, SARS, and Ebola: The Role of Super-Spreaders in Infectious Disease. Cell Host & Microbe 18 (4): 398-401. doi: 10.1016/j. chom.2015.09.013
  • Zheng J (2020). SARS-CoV-2: An emerging coronavirus that causes a global threat. International Journal of Biological Sciences 16 (10): 1678-1685. /doi: 10.7150/ijbs.45053
  • Zhou P, Yang XL, Wang XG, Hu B, Zhang L et al. (2020). A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579 (7798): 270-273. /doi: 10.1038/s41586-020- 2012-7
APA Tastan C, yurtsever b, Sır G, DİLEK KANÇAĞI D, Demir S, Abanuz S, Seyis U, yıldırım m, Kuzay R, Elibol O, Arbak S, Acikel Elmas M, Birdoğan S, Sezerman O, Kocagöz S, Yalçın K, Ovalı E (2020). SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. , 192 - 202. 10.3906/biy-2004-113
Chicago Tastan Cihan,yurtsever bulut,Sır Gözde,DİLEK KANÇAĞI Derya,Demir Sevda,Abanuz Selen,Seyis Utku,yıldırım mülazim,Kuzay Recai,Elibol Omer,Arbak Serap,Acikel Elmas Merve,Birdoğan Selçuk,Sezerman Osman Ugur,Kocagöz Sesin,Yalçın Koray,Ovalı Ercüment SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. (2020): 192 - 202. 10.3906/biy-2004-113
MLA Tastan Cihan,yurtsever bulut,Sır Gözde,DİLEK KANÇAĞI Derya,Demir Sevda,Abanuz Selen,Seyis Utku,yıldırım mülazim,Kuzay Recai,Elibol Omer,Arbak Serap,Acikel Elmas Merve,Birdoğan Selçuk,Sezerman Osman Ugur,Kocagöz Sesin,Yalçın Koray,Ovalı Ercüment SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. , 2020, ss.192 - 202. 10.3906/biy-2004-113
AMA Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. . 2020; 192 - 202. 10.3906/biy-2004-113
Vancouver Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. . 2020; 192 - 202. 10.3906/biy-2004-113
IEEE Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E "SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients." , ss.192 - 202, 2020. 10.3906/biy-2004-113
ISNAD Tastan, Cihan vd. "SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients". (2020), 192-202. https://doi.org/10.3906/biy-2004-113
APA Tastan C, yurtsever b, Sır G, DİLEK KANÇAĞI D, Demir S, Abanuz S, Seyis U, yıldırım m, Kuzay R, Elibol O, Arbak S, Acikel Elmas M, Birdoğan S, Sezerman O, Kocagöz S, Yalçın K, Ovalı E (2020). SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. Turkish Journal of Biology, 44(3), 192 - 202. 10.3906/biy-2004-113
Chicago Tastan Cihan,yurtsever bulut,Sır Gözde,DİLEK KANÇAĞI Derya,Demir Sevda,Abanuz Selen,Seyis Utku,yıldırım mülazim,Kuzay Recai,Elibol Omer,Arbak Serap,Acikel Elmas Merve,Birdoğan Selçuk,Sezerman Osman Ugur,Kocagöz Sesin,Yalçın Koray,Ovalı Ercüment SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. Turkish Journal of Biology 44, no.3 (2020): 192 - 202. 10.3906/biy-2004-113
MLA Tastan Cihan,yurtsever bulut,Sır Gözde,DİLEK KANÇAĞI Derya,Demir Sevda,Abanuz Selen,Seyis Utku,yıldırım mülazim,Kuzay Recai,Elibol Omer,Arbak Serap,Acikel Elmas Merve,Birdoğan Selçuk,Sezerman Osman Ugur,Kocagöz Sesin,Yalçın Koray,Ovalı Ercüment SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. Turkish Journal of Biology, vol.44, no.3, 2020, ss.192 - 202. 10.3906/biy-2004-113
AMA Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. Turkish Journal of Biology. 2020; 44(3): 192 - 202. 10.3906/biy-2004-113
Vancouver Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients. Turkish Journal of Biology. 2020; 44(3): 192 - 202. 10.3906/biy-2004-113
IEEE Tastan C,yurtsever b,Sır G,DİLEK KANÇAĞI D,Demir S,Abanuz S,Seyis U,yıldırım m,Kuzay R,Elibol O,Arbak S,Acikel Elmas M,Birdoğan S,Sezerman O,Kocagöz S,Yalçın K,Ovalı E "SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients." Turkish Journal of Biology, 44, ss.192 - 202, 2020. 10.3906/biy-2004-113
ISNAD Tastan, Cihan vd. "SARS-CoV-2 isolation and propagation from Turkish COVID-19 patients". Turkish Journal of Biology 44/3 (2020), 192-202. https://doi.org/10.3906/biy-2004-113