Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells
Yıl: 2022 Cilt: 46 Sayı: 3 Sayfa Aralığı: 216 - 226 Metin Dili: İngilizce DOI: 10.55730/1300-0152.2609 İndeks Tarihi: 07-07-2022
Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells
Öz: Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Hamid S, Akgül B (2022). Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. , 216 - 226. 10.55730/1300-0152.2609 |
| Chicago | Hamid Syed,Akgül Bünyamin Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. (2022): 216 - 226. 10.55730/1300-0152.2609 |
| MLA | Hamid Syed,Akgül Bünyamin Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. , 2022, ss.216 - 226. 10.55730/1300-0152.2609 |
| AMA | Hamid S,Akgül B Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. . 2022; 216 - 226. 10.55730/1300-0152.2609 |
| Vancouver | Hamid S,Akgül B Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. . 2022; 216 - 226. 10.55730/1300-0152.2609 |
| IEEE | Hamid S,Akgül B "Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells." , ss.216 - 226, 2022. 10.55730/1300-0152.2609 |
| ISNAD | Hamid, Syed - Akgül, Bünyamin. "Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells". (2022), 216-226. https://doi.org/10.55730/1300-0152.2609 |
| APA | Hamid S, Akgül B (2022). Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology, 46(3), 216 - 226. 10.55730/1300-0152.2609 |
| Chicago | Hamid Syed,Akgül Bünyamin Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology 46, no.3 (2022): 216 - 226. 10.55730/1300-0152.2609 |
| MLA | Hamid Syed,Akgül Bünyamin Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology, vol.46, no.3, 2022, ss.216 - 226. 10.55730/1300-0152.2609 |
| AMA | Hamid S,Akgül B Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology. 2022; 46(3): 216 - 226. 10.55730/1300-0152.2609 |
| Vancouver | Hamid S,Akgül B Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells. Turkish Journal of Biology. 2022; 46(3): 216 - 226. 10.55730/1300-0152.2609 |
| IEEE | Hamid S,Akgül B "Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells." Turkish Journal of Biology, 46, ss.216 - 226, 2022. 10.55730/1300-0152.2609 |
| ISNAD | Hamid, Syed - Akgül, Bünyamin. "Cytoplasmically localized tRNA-derived fragments inhibit translation in Drosophila S2 cells". Turkish Journal of Biology 46/3 (2022), 216-226. https://doi.org/10.55730/1300-0152.2609 |
