TY  - JOUR
TI  - Cytoplasmically localized tRNA-derived fragments inhibit translation
in Drosophila S2 cells
AB  - Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in Drosophila S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in Drosophila S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5’-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in Drosophila.
AU  - Akgül, Bünyamin
AU  - Hamid, Syed
DO  - 10.55730/1300-0152.2609
PY  - 2022
JO  - Turkish Journal of Biology
VL  - 46
IS  - 3
SN  - 1300-0152
SP  - 216
EP  - 226
DB  - TRDizin
UR  - http://search/yayin/detay/532172
ER  -