TY  - JOUR
TI  - Th e Eff ects of Fast and Slow Th awing on Spermatological Parameters
and Detect of Chromatin Condensation by Toluidine Blue Staining
in Frozen-Th awed Bull Sperm
AB  - Th e purpose of this study was to observe the eff ect of diff erent thawing methods on semen parameters such as motility and morphology
and sperm chromatin integrity as assessed by toluidine blue (TB) staining. A total of 20 frozen sperm straws from the same Holstein bull were
used. While the 30 sec thawing protocol at 37°C, which is used for thawing frozen sperm straws, constitutes our slow thawing group (n=10),
the 6 sec thawing protocol at 70°C constitutes our fast-thawing group (n=10). Th e motility, viability, morphology, plasma membrane integrity,
and sperm chromatin condensation parameters of all thawed sperm were investigated. Th ere was a significant diff erence (P<0.05) in sperm
plasma membrane integrity, head defect, and total abnormal sperm morphology. Th e chromatin decondensation rate detected by TB in bull
semen thawed in the slow thaw system, and the decondensation rate in the fast thaw system, diff ered significantly from each other in line with
the literature data (P<0.05). According to the evaluations made in terms of chromatin decondensation rate, the rate obtained in slow thawing
(7±0.39) shows an increase up to two times compared to the fast-thawing rate (3.3±0.33) (P<0.05). Th e TB staining procedure can be used to
evaluate infertility and chromatin integrity, especially in cases that are suspicious and require rapid evaluation.
AU  - ESİN, Burcu
AU  - CEVIK, MESUT
AU  - AKAR, MELİH
AU  - KAYA, Cumali
DO  - 10.9775/kvfd.2021.26950
PY  - 2022
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 28
IS  - 3
SN  - 1300-6045
SP  - 307
EP  - 313
DB  - TRDizin
UR  - http://search/yayin/detay/532192
ER  -