Stem cell approaches has been increasing in the conventional medicine. Th is study was planned to determinate the efficacy of adipose-derived stem cells (ADSCs) on the bone fracture’s regeneration by histologically, morphologically and radiologically. Primarily isolated cells were cultivated and identified for the obtaining of the third passage mesenchymal stem cells in the cell culture laboratory. Th e right tibias (applicated ADSCs) were planned as experimental group and the left tibias were studied as control on the clinically healthy 10 rats. Th e middle diaphysis of the right and left tibias was transversally cut by a thin saw and then fixed by intramedullary pin. Before surgical closure of the incised area, 106 ADSCs were injected on an absorbent material, which covered the cut site of the right tibia, in experimental group. At postoperative 45th day, cranio-caudal and lateral radiographs of the tibias pointed out that there was callus formation in the caudal and lateral parts of the right tibia. After sacrification of the animals, preparations were made for histological examinations. Th e results indicate that there were clear diff erences in the mitotic activity; and also, the experimental tibias were found to be rich in blood vessel network. As a conclusion, it was found that ADSCs can have a positive potential effect on osteogenesis in bone tissue.