TY  - JOUR
TI  - Effect of α-lipoic acid and N-acetylcysteine on liver oxidative stress,
preneoplastic lesions induced by diethylnitrosamine plus high-fat diet
AB  - Aim: Oxidative stress and inflammation are important for development of nonalcoholic steatohepatitis (NASH),
cirrhosis and hepatocellular carcinoma (HCC). High fat diet (HFD) acts as promoter and induces cancer
formation by diethylnitrosamine (DEN)-initiated carcinogenesis. DEN+HFD experimental model may be
suitable to investigate the relationship between diet, cirrhosis, and cancer.
Methods: Rats were injected with DEN (50 mg/kg/once a week; i.p.) for 4 weeks. After 15 days, rats received
HFD with/without supplementations of α-lipoic acid (ALA; 2 g/kg chow), N-acetylcysteine (NAC; 1% w/v
drinking water) and their combination for 12 weeks.
Results: DEN+HFD-treatment resulted in increase of serum hepatic damage markers, hepatic oxidative stress
parameters (lipid/protein oxidation products) and fibrotic changes. However, no HCC nodule was detected.
Hepatic GST-pi and Ki-67 expressions also increased. Accordingly, DEN+HFD-treatment resulted in
precancerous lesions and high rate of proliferation in the liver. NAC supplementation decreased hepatic
oxidative stress and formation of fibrotic and preneoplastic lesions of DEN+HFD-treated rats. However, ALA
supplementation did not have a curative effect on these lesions. No synergistic effect was seen with coadministration
of ALA and NAC.
Conclusion: According to present results NAC, acting as an antioxidant, has ameliorating effect on DEN+HFDinduced
oxidative stress and the formation of preneoplastic lesions in liver.
AU  - Soluk Tekkesin, Merva
AU  - Uysal, Mujdat
AU  - Dogru-Abbasoglu, Semra
AU  - Aydın, A. Fatih
AU  - Baki, Adile merve
AU  - Vural, Pervin
DO  - 10.25000/acem.830126
PY  - 2021
JO  - Archives of Clinical and Experimental Medicine
VL  - 6
IS  - 1
SN  - 2564-6567
SP  - 1
EP  - 8
DB  - TRDizin
UR  - http://search/yayin/detay/532714
ER  -